金黄色葡萄球菌SarA家族蛋白及其翻译后修饰

摘要/Abstract

摘要： 在金黄色葡萄球菌全基因组中发现了一类SarA 类似的蛋白家族，是金黄色葡萄球菌生理和毒力主要的调控因子。MgA 、SarA 和SarZ 等蛋白分子中半胱氨酸的磷酸化修饰或氧化还原修饰引起了蛋白质构象的变化，从而调节了金黄色葡萄球菌毒力因子的产生以及细菌对万古霉素的抗性。SarA 家族蛋白具有同源性，蛋白翻译后修饰具有相似性，同时蛋白之间又相互作用。

关键词: SarA 家族蛋白, 磷酸化, 氧化还原修饰, MgrA, SarZ

Abstract: A class of family proteins found in staphylococcus aureus genome is similar to SarA, it contributes to the main physiological and virulence of staphylococcus aureus. protein cysteine phosphorylation of MgA/SarA/SarZ family or Oxidation and reduction modification can caused the change of protein conformation, which regulates the virulence factor of S. aureus and to vancomycin-resistant. SarA family proteins are homologous and interacted, and its post-translational modification is similar.

Key words: Family proteins of SarA, Phosphorylation, Oxidation and reduction modification, MgrA, SarZ

吴睿睿,朱福兴. 金黄色葡萄球菌SarA家族蛋白及其翻译后修饰[J]. 生物技术通报, 2013, 0(10): 40-45.

Wu Ruirui Zhu Fuxing. SarA Family Proteins and Its Post-Translational Modification in Staphylococcus aureus [J]. Biotechnology Bulletin, 2013, 0(10): 40-45.

参考文献

[1]Hecker M, Engelmann S, Cordwell SJ. Proteomics of Staphylococcus aureus current state and future chal lenges[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2003, 787（1）：179-195.
[2]Cheung AL, Bayer AS, Zhang G, et al. Regulation of virulence determinants in vitro and in vivo in Staphylococcus aureus[J]. FEMS Immunol Med Microbiol, 2004, 40（1）：1-9.
[3]Cheung AL, Nishina KA, Trotonda MP, et al. The SarA protein family of Staphylococcus aureus[J]. The International Journal of Biochemistry & Cell Biology, 2008, 40（3）：355-361.
[4]Bronner S, Monteil H, Prevost G. Regulation of virulence determinants in Staphylococcus aureus ：Complexity and applications[J]. FEMS Microbiol Rev, 2004, 28（2）：183-200.
[5]Luong TT, Newell SW, Lee CY. Mgr, a novel global regulator in Staphylococcus aureus[J]. J Bacteriol, 2003, 185（13）：3703-3710.
[6]Li R, Manna AC, Dai S, et al. Crystal structure of the SarS protein from Staphylococcus aureus[J]. J Bacteriol, 2003, 185（14）： 4219-4225.
[7]Oscarsson J, Harlos C, Arvidson S. Regulatory role of proteins binding to the spa（protein A）and sarS（staphylococcal accesso-ryregulator）promoter regions in Staphylococcus aureus NTCC 8325-4 [J]. Int J Med Microbiol, 2005, 295（4）：253-266.
[8]Safo MK, Zhao Q, Ko TP, et al. Crystal structures of the BlaI repressor from Staphylococcus aureus and its complex with DNA ：insights into transcriptional regulation of the bla and mec operons[J]. J Bacteriol, 2005, 187 ：1833-1844.
[9]Chien CT, Manna AC, Projan SJ, et al. SarA, a global regulator of virulence determinants in Staphylococcus aureus, binds to a conserved motif essential for sar-dependent gene regulation[J]. J Biol Chem, 1999, 274 ：37169-37176.
[10]Manna AC, Bayer MG, Cheung AL. Transcriptional analysis of different promoters in the sar locus in Staphylococcus aureus[J]. J Bacteriol, 1998, 180 ：3828-3836.
[11]Cheung AL, Nishina K, Manna AC. SarA of Staphylococcus aureus binds to the sarA promoter to regulate gene expression[J]. Journal of Bacteriology, 2008, 190（6）：2239-2243.
[12]Manna AC, Cheung AL. Transcriptional regulation of the agr locus and the identification of DNA binding residues of the global regulatory protein SarR in Staphylococcus aureus[J]. Mol Microbiol, 2006, 60（5）：1289-301.
[13]Frees D, Sorensen K, Ingmer H. Global virulence regulationin Staphylococcus aureus ：pinpointing the roles of ClpP and ClpX in the sar/agr regulatory network[J]. Infect Immun, 2005, 73（12）： 8100-8108.
[14]Cheung AL, Nishina KA, Trotonda MP, et al. The SarA protein family of Staphylococcus aureus[J]. Int J Biochem Cell Biol, 2008, 40（3）：355-361.
[15]Truong-Bolduc QC, Dunman PM, Strahilevitz J, et al. MgrA is a multiple regulator of two new efflux pumps in Staphylococcus aureus[J]. J Bacteriol, 2005, 187 ：2395-2405.
[16]Paget MS. Buttner MJ. Thiol-based regulatory switches[J]. Annu Rev Genet, 2003, 37 ：91-121.
[17]Stamler JS, Lamas S, Fang FC. Nitrosylation ：the prototypic redox-based signaling mechanism[J]. Cell, 2001, 106 ：675-683.
[18]Poole LB, Karplus PA, Claiborne A. Protein sulfenic acids in redox signaling[J]. Annu Rev Pharmacol Toxicol, 2004, 44 ：325-347.
[19]Lee JW, Helmann JD. The PerR transcription factor senses H2O2 by metalcatalysedhistidine oxidation[J]. Nature, 2006, 440 ：363367.
[20]Sun F, Ji Q, Lan L, et al. Transcriptional regulators mediates bacterial virulence and antibiotic resistance[J]. Proc Natl Acad Sci USA, 2012, 109（38）：15461 -15466.
[21]Chen PR, Bae T, Williams WA, et al. An oxidation-sensing mechanism is used by the global regulator MgrA in Staphylococcus aureus[J]. Nature, 2006, 2 ：591-595.
[22]Kaito C, Morishita D, Matsumoto Y, et al. Novel DNA binding p rot e i n S ar Z c o n tr i b u t e s to v i ru l e nc e in S t a p h ylo c o c c us aureus[J]. Molecular Microbiology, 2006, 62（6）：1601-1617.
[23]Peng HL, Novick RP, Kreiswirth B, et al. Cloning, characterization, and sequencing of an accessory gene regulator（agr）in Staphylococcus aureus[J]. J Bacteriol, 1988, 170 ：4365-4372.
[24]Rechtin TM, Gillaspy AF, Schumacher MA, et al. Characterization of the SarA virulence gene regulator of Staphylococcus aureus[J]. Mol Microbiol, 1999, 33 ：307-316.
[25]Shi L, Takahashi K, Dundee J, et al. Mannose-binding lectindeficient mice are susceptible to infection with Staphylococcus aureus [J]. J Exp Med, 2004, 199 ：1379-1390.
[26]Ghezzi P, Bonetto V. Redox proteomics ：Identificat ion of oxidatively modified proteins[ J]. Proteomics, 2003, 3 ：1145-1153.
[27]Lee K, Lee J, Kim Y, et al . Defining the plant disulfide proteo-me[J]. Electrophoresis, 2004, 25 ：532-541.
[28]Hunter T, Sefton BM. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine[J]. Proc Natl Acad Sci USA, 1980, 77 ：1311-1315.
[29]Tarrant MK, Cole PA. The chemical biology of protein phosphoryla-tion[J]. Annu Rev Biochem, 2009, 78 ：797-825.
[30] 刘金凤, 王京兰, 钱小红, 蔡耘. 翻译后修饰蛋白质组学研究的技术策略[J] . 中国生物化学与分子生物学报, 2007, V23 （2）：93-100, 301-305.
[31]Krupa A, Preethi G, Srinivasan N. Structural modes of stabilization of permissive phosphorylation sites in protein kinases ：distinct strategies in Ser/Thr and Tyr kinases[J]. J Mol Biol, 2004, 339 ： 1025-1039.
[32]Walsh CT, Garneau-Tsodikova S, Gatto GJ Jr. Protein posttranslational mod-ifications ：The chemistry of proteome diversificati-ons[J]. Angew Chem Int Ed Engl, 2005, 44 ：7342-7372.