一株抗根结线虫放线菌的筛选与鉴定

生物技术通报 ›› 2013, Vol. 0 ›› Issue (11): 176-180.

一株抗根结线虫放线菌的筛选与鉴定

黄惠琴¹, 袁维道^1，2, 魏华^1，2, 王英^1，2, 朱军¹, 孙前光¹, 鲍时翔¹

1.中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室，海口 571101；2.海南大学环境与植物保护学院，海口 570228

收稿日期:2013-05-07 出版日期:2013-11-14 发布日期:2013-11-14
作者简介:黄惠琴，副研究员，研究方向：微生物资源与利用；E-mail：huanghq21@163.com；袁维道为并列第一作者
基金资助:
“973”计划项目(2012CB017104)，国家自然科学基金项目(31170062)，海南大学211工程建设项目，海南大学环境与植物保护学院研究生创新平台

Screening and Identification of an Actinomycete Strain with Nematicidal Activity

Huang Huiqin¹, Yuan Weidao^1，2, Wei Hua^1，2, Wang Ying^1，2, Zhu Jun¹, Sun Qianguang¹, Bao Shixiang¹

(1. Key Laboratory of Biology and Genetic Resources of Tropical Crops of Agriculture Ministry，Institute of Tropical Bioscience and Biotechnology，CATAS，Haikou 571101；2. College of Environment and Plant Protection，Hainan University，Haikou 570228)

Received:2013-05-07 Published:2013-11-14 Online:2013-11-14

摘要/Abstract

摘要： 从海南省东寨港红树林底泥中分离获得具有抗线虫活性的放线菌菌株HA11097。将该菌株发酵液稀释20倍和40倍时，抗根结线虫校正死亡率分别为54.2%和46.7%。16S rRNA基因序列分析表明，菌株HA11097属于链霉菌属(Streptomyces)中的成员，与S. aculeolatus的相似性最高，为98.9%。二者在系统发育树上处于同一分支，亲缘关系最近，DNA-DNA杂交值为94.4%。该菌的形态特征、培养特征以及生理生化特征与S. aculeolatus也基本一致。综合以上结果，鉴定菌株HA11097为针孢链霉菌Streptomyces aculeolatus，首次报道了其抗线虫活性。

关键词: 红树林, 放线菌, 根结线虫, 鉴定

Abstract: Root-knot nematode antagonistic strain HA11097 was isolated from mud samples collected from Dongzhaigang mangroves in Hainan province. The mortality rates of the fermentation broth against root-knot nematode were 54.2% and 46.7% while diluted 20-fold and 40-fold, respectively. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain HA11097 should belong to a member of the genus Streptomyces, and closely related to S. aculeolatus, with the highest 16S rRNA gene similarity of 98.9%. In the phylogenetic tree, HA11097 was the closest relationship with S. aculeolatus, which both located on the same branch. Their DNA-DNA hybridization value was 94.4%. The morphological, cultural characteristics and the results of physiological and biochemical properties of the strain were in well agreement with S. aculeolatus. On the basis of these data, strain HA11097 was identified as Streptomyces aculeolatus. The article first reported that the strain of anti-nematode activity.

Key words: Mangrove, Actinomycetes, Root-knot nematodes, Identification

黄惠琴, 袁维道, 魏华, 王英, 朱军, 孙前光, 鲍时翔. 一株抗根结线虫放线菌的筛选与鉴定[J]. 生物技术通报, 2013, 0(11): 176-180.

Huang Huiqin, Yuan Weidao, Wei Hua, Wang Ying, Zhu Jun, Sun Qianguang, Bao Shixiang. Screening and Identification of an Actinomycete Strain with Nematicidal Activity[J]. Biotechnology Bulletin, 2013, 0(11): 176-180.

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