牦牛A-FABP基因的克隆与生物信息学分析

生物技术通报 ›› 2013, Vol. 0 ›› Issue (11): 86-92.

牦牛A-FABP基因的克隆与生物信息学分析

秦文^1，2，3, 阎萍^1，2，3, 裴杰^2，3, 吴晓云^2，3, 李天科^1，2，3

1.甘肃农业大学动物科学技术学院，兰州 730070；2.中国农业科学院兰州畜牧与兽药研究所，兰州 730050；3.甘肃省牦牛繁育工程重点实验室，兰州 730050

收稿日期:2013-05-02 出版日期:2013-11-14 发布日期:2013-11-14
作者简介:秦文，女，硕士研究生，研究方向：动物遗传育种; E-mail：qinwen_614@163.com
基金资助:
国家肉牛牦牛产业技术体系牦牛选育(nycytx-38)，甘肃牧区生产生态生活优化保障技术集成示范项目(2012BAD13B05)

Cloning and Bioinformatics Analysis of A-FABP Gene in Yak

Qin Wen^1，2，3, Yan Ping^1，2，3, Pei Jie^2，3, Wu Xiaoyun^2，3, Li Tianke^1，2，3

(1. Faculty of Animal Sci-tech Gansu Agricultural University，Lanzhou 730070；2. Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS，Lanzhou 730050；3. Key Laboratory of Yak Breeding Engineering，Lanzhou 730050)

Received:2013-05-02 Published:2013-11-14 Online:2013-11-14

摘要/Abstract

摘要： 以牦牛的脂肪型脂肪酸结合蛋白(A-FABP)基因为研究对象，根据GenBank收录的牛的A-FABP基因的mRNA序列设计一对特异性引物，以牦牛背最长肌为材料，采用RT-PCR方法克隆牦牛A-FABP基因的CDS区，并对其进行生物信息学分析。结果表明，牦牛A-FABP基因的CDS区全长399 bp，编码 132个氨基酸；其编码的蛋白属于亲水性蛋白。二级结构主要由α-螺旋、β-折叠、延伸链以及无规卷曲组成，含有11个磷酸化位点。牦牛A-FABP基因编码的氨基酸序列与黄牛和水牛的同源性最高且都为97%，与其他物种的同源性也较高，说明在进化关系上A-FABP氨基酸序列较保守，此蛋白序列具Cytosolic fatty-acid binding 家族蛋白功能结构域，无信号肽。

关键词: 牦牛, A-FABP基因, 克隆, 生物信息学分析

Abstract: A-FABP(Adipocyte Fat Acid Binding Protein)gene of yak was used to design a pair of specific primer based on the A-FABP mRNA sequences included in NCBI GeneBank. The CDS sequences of yak A-FABP gene was cloned by reverse transcription polymerase chain reaction(RT-PCR)from yak longissimus dorsi muscle, and were analyzed. The results showed that, the length of CDS in yak A-FABP mRNA was 399 bp and encoding 132 amino acid, which encoded a hydrophilic protein. The secondary structures were mainly composed of the helix, β turn, extended strand and random coil, containing 11 phosphorylation sites. The A-FABP and realated proteins of Bos grunnien, Bos taurus and Bubalus bubalis shared the highest homology of 97%. A-FABP amino acid sequences are preferably conserved on the evolutionary relationships, and also showed high homology of with other species. The A-FABP features cytosolic fatty-acid binding family protein functional domains, no signal peptide.

Key words: Yak, A-FABP, Gene, Cloning, Bioinformatic analysis

秦文, 阎萍, 裴杰, 吴晓云, 李天科. 牦牛A-FABP基因的克隆与生物信息学分析[J]. 生物技术通报, 2013, 0(11): 86-92.

Qin Wen, Yan Ping, Pei Jie, Wu Xiaoyun, Li Tianke. Cloning and Bioinformatics Analysis of A-FABP Gene in Yak[J]. Biotechnology Bulletin, 2013, 0(11): 86-92.

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