天祝白牦牛GDF-10基因CDS区的多态性及生物信息学分析

生物技术通报 ›› 2013, Vol. 0 ›› Issue (11): 79-85.

天祝白牦牛GDF-10基因CDS区的多态性及生物信息学分析

李天科^1，2，3, 梁春年^2，3, 郎侠^2，3, 裴杰^2，3, 吴晓云^2，3, 刘建^2，3, 秦文^1，2，3, 阎萍^1，2，3

1.甘肃农业大学动物科学技术学院，兰州 730070；2. 中国农业科学院兰州畜牧与兽药研究所，兰州 730050；3.甘肃省牦牛繁育工程重点实验室，兰州 730050

收稿日期:2013-04-22 出版日期:2013-11-14 发布日期:2013-11-14
作者简介:李天科，男，硕士研究生，研究方向：动物遗传育种与繁殖；E-mail：litianke1987@163.com
基金资助:
科技部科技支撑计划(2012BAD13B05)，甘肃省科技重大专项计划(1102NKDA027)

Polymorphism and Bioinformatics Analysis of CDS in GDF-10 Gene in Tianzhu White Yak

Li Tianke^1，2, Liang Chunnian^2，3, Lang Xia^2，3, Pei Jie^2，3, Wu Xiaoyun^2，3, Liu Jian^2，3, Qin Wen^1，2，3, Yan Ping^1，2，3

(1. College of Animal Science and Technolog，Gansu Agricultural University，Lanzhou 730070；2. Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences，Lanzhou 730050；3. Key Laboratory of Yak Breeding Engineering，Lanzhou 730050)

Received:2013-04-22 Published:2013-11-14 Online:2013-11-14

摘要/Abstract

摘要： 采用DNA混合池测序法检测天祝白牦牛生长分化因子-10(Growth differentiation factor 10，GDF-10)基因编码区的多态性，应用生物信息学方法分析天祝白牦牛生长分化因子-10编码蛋白质特性。结果表明，天祝白牦牛生长分化因子-10基因编码区序列存在8个突变位点，其中90C→G、124C→G、1005G→A和1232G→C为错义突变，导致了编码氨基酸的改变，132T→C、822A→G、873G→T和888T→C为同义突变；天祝白牦牛生长分化因子-10编码蛋白质的氨基酸序列没有明显的疏水性区域和跨膜螺旋区；其存在信号肽，说明可能在细胞质中发挥生物学作用；天祝白牦牛生长分化因子-10编码产物二级结构是以α-螺旋和β-折叠为主；氨基酸序列与普通牛的同源性为99.4%，与人、小鼠、大鼠4个物种间同源性较高。

关键词: 天祝白牦牛, GDF-10基因, 多态性, 生物信息学分析

Abstract: Mutation site in coding domain sequence of GDF-10(Growth differentiation factor -10)gene in Tianzhu white yak was checked by DNA pooling method, and the polymorphism and the characters of amino acid sequence were analyzed by bioinformatics. The results showed there were eight mutation(90C→G, 124C→G, 132T→C, 822A→G, 873G→T, 888T→C, 1005G→A, 1232G→C)at sequence of CDS, four synonymous mutation(90C→G, 124C→G, 1005G→A, 1232G→C)and four missense mutation(132T→C, 822A→G, 873G→T, 888T→C). NO obvious hydrophobic and transmembrane helical region, signal peptide was found in deduced amino acid sequence. GDF-10 proteins would play biological function mainly in the cytoplasm. The putative secondary structure was mixed type primarily composed of α-helix and β-extended. The deduce amino acid sequence of GDF-10 between yak and bovine, mouse and rat were high homology, which was same as their genetic relationship.

Key words: Tuanzhu white yak, GDF-10 gene, Polyorphism, Bioinformatic

李天科, 梁春年, 郎侠, 裴杰, 吴晓云, 刘建, 秦文, 阎萍. 天祝白牦牛GDF-10基因CDS区的多态性及生物信息学分析[J]. 生物技术通报, 2013, 0(11): 79-85.

Li Tianke, Liang Chunnian, Lang Xia, Pei Jie, Wu Xiaoyun, Liu Jian, Qin Wen, Yan Ping. Polymorphism and Bioinformatics Analysis of CDS in GDF-10 Gene in Tianzhu White Yak[J]. Biotechnology Bulletin, 2013, 0(11): 79-85.

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