自养条件下高氯酸盐降解细菌群落研究

摘要/Abstract

摘要： 旨在研究自养条件下以氢气作为电子供体高氯酸根离子(ClO_{4-)的微生物降解机制，利用HiSeq 2000对微生物群落结构及多样性进行高通量测序及分析。结果表明，添加氢气的HD(hydrogen degradation)体系将10 mg/L ClO_{4-降至检出限以下共经历71 d。ClO_{4-完全降解后HD体系中总细菌的相对丰度为84.96%，绝大多数降解细菌分布的变形菌门相对丰度为68.11%，占总细菌数的比例达到80.16%。其中属于β-变形杆菌纲的ClO_{4-代表性降解细菌(perchlorate-reducing bacteria，PRB)Dechloromonas的相对丰度为2.7%，另一种Azospira为3.1%。通过KEGG注释对菌种功能进行分析。参与糖代谢的基因相对丰度为4.75%，参与能量代谢的基因相对丰度为3.35%，其中参与氮循环的基因相对丰度为0.72%，参与氯代物降解和氯循环的基因相对丰度为0.83%，补充氢气对反应体系内生物群落结构变化起到选择纯化作用，使优势菌群趋于单一。}}}}

关键词: 高氯酸根离子(ClO_4-), 电子供体, 氢气, 细菌群落结构, 高通量测序

Abstract: For the purpose of further investigating the biological degradation under an autotrophic condition and well understanding the microbial community structures in a complex environment, hydrogen was used as an electron donor to completely reduce perchlorate(ClO_{4-)in this study. The composition of microbial communities after degradation was analyzed via the construction of a cloning library by using the High-Throughput Sequencing method(HiSeq 2000). 71 days were needed to completely degrade 10 mg/L ClO_{4-. Microbial phylogenic analysis of HD(hydrogen degradation)after degradation indicated that the relative abundance of total bacteria in the HD was 84.96% whereas the relative abundance of Proteobacteria was 68.11%, whose percentage accounting for the total bacteria reached to 80.16%. The relative abundance of Dechloromonas which is representative in PRB was 2.7% in the HD. Simultaneously, the relative abundance of Azospira was 3.1%. KEGG was used to analyze the function of bacteria in HD. The relative abundance of genes which engaged in carbohydrate metabolism was 4.75%, and the genes included in energy metabolism was 3.35%, whereas the genes participated in nitrogen cycle was 0.72%, and the genes involving chloride transformation was 0.83%. It was demonstrated that degradation of ClO_{4- in a complicated condition was achieved by various kinds of microbes rather than a single one. Adding hydrogen as an electron donor to change microbial community played a role in the purification or selection process in the system, which allowed the complex systems to have the specific capacity to remove given contaminates.}}}

Key words: Perchlorate(ClO_4-), Electron donor, Hydrogen, The composition of microbial communities, High-Throughput Sequencing

谢宇轩, 关翔宇, 于丽莎, 刘菲. 自养条件下高氯酸盐降解细菌群落研究[J]. 生物技术通报, 2014, 0(4): 169-175.

Xie Yuxuan, Guan Xiangyu, Yu Lisha, Liu Fei,. The Study of the Structure of Perchlorate(ClO^4-)-degrading Bacterial Communities Under Autotrophic Conditions[J]. Biotechnology Bulletin, 2014, 0(4): 169-175.

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