杀真菌素链霉菌AL-04的筛选与鉴定

生物技术通报 ›› 2014, Vol. 0 ›› Issue (5): 155-161.

杀真菌素链霉菌AL-04的筛选与鉴定

李增波孙红艳赵静娴周剑武梁栋高晔

(太原科技大学化学与生物工程学院，太原 030021)

收稿日期:2013-12-19 出版日期:2014-05-23 发布日期:2014-05-24
作者简介:李增波，男，讲师，研究方向：微生物资源及应用；E-mail：lizengbo@sina.com
基金资助:
太原科技大学青年基金项目(20113008)，太原科技大学"UIT"计划(2012)

Screening and Identification of Streptomyces fungicidicus Strain AL-04

Li Zengbo Sun Hongyan Zhao Jingxian Zhou Jianwu Liang Dong Gao Ye

(College of Chemical and Biological Engineering，Taiyuan Sci-Tech University，Taiyuan 030021)

Received:2013-12-19 Published:2014-05-23 Online:2014-05-24

摘要/Abstract

摘要： 对13株来源于青藏高原土壤的放线菌进行活化，再分别采用琼脂扩散法、生长速率法、孢子萌发抑制法和离体叶片法筛选出一株抗菌活性高且抗性稳定的菌株AL-04。抗菌活性结果表明，该菌株对4种常见的土传病害病原真菌：辣椒疫霉病菌(Phytophthora capsici)、黄瓜枯萎病菌(Fusarium oxysporum f. sp. cucumerinum)、西瓜枯萎病菌(Fusarium oxysporum f. sp. niveum)、番茄灰霉病菌(Botrytis cinerea)都有较强的抑制效果，抑菌率均在70.0%以上，其中对辣椒疫霉病菌(Phytophthora capsici)抑菌率高达93.0%。通过形态特征、生理生化特征分析和16S rDNA序列分析，将该菌株鉴定为杀真菌素链霉菌(Streptomyces fungicidicus)。

关键词: 放线菌, 生物防治, 防病效果, 鉴定, 16S, rDNA

Abstract: 13 actinomycetes strains were screened from soil samples of Qinghai-Tibet Plateau. Strain AL-04 which has high and stable inhibition effects on soilborne pathogens was selected by methods of agar diffusion, relative growth rate, spore germination inhibition and vegetable leaves. Results of antagonistic activity showed that AL-04 had strong antagonistic activity against 4 species of soilborne pathogens including Phytophthora capsici, Fusarium oxysporum f. sp. cucumerinum, Fusarium oxysporum f. sp. niveum and Botrytis cinerea. The inhibition rates were beyond 70.0% for all pathogens, of which 93.0% was observed for Phytophthora capsici. AL-04 was identified as Streptomyces fungicidicus by the observation of the morphological features, cytochemical characters and 16S rDNA sequence.

Key words: Actinomycetes Biocontrol, Biocontrol efficacy, Identification 16S, RDNA

李增波, 孙红艳, 赵静娴, 周剑武, 梁栋, 高晔. 杀真菌素链霉菌AL-04的筛选与鉴定[J]. 生物技术通报, 2014, 0(5): 155-161.

Li Zengbo, Sun Hongyan, Zhao Jingxian, Zhou Jianwu, Liang Dong, Gao Ye. Screening and Identification of Streptomyces fungicidicus Strain AL-04[J]. Biotechnology Bulletin, 2014, 0(5): 155-161.

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