早胜牛类群A-FABP基因SNPs及其与胴体品质和肉质性状的相关性分析

生物技术通报 ›› 2014, Vol. 0 ›› Issue (5): 96-101.

早胜牛类群A-FABP基因SNPs及其与胴体品质和肉质性状的相关性分析

唐红杰赵生国雷赵民王欣荣王建福蔡原吴建平

(甘肃农业大学动物科学技术学院，兰州 730070)

收稿日期:2013-10-17 出版日期:2014-05-23 发布日期:2014-05-24
作者简介:唐红杰，女，硕士研究生，研究方向：动物遗传育种与繁殖；E-mail：tang.071@163.com
基金资助:
甘肃省农业生物技术研究与应用开发项目(GNSW-2010-04，GNSW-2011-27)，甘肃省农业科技创新项目，甘肃省高等学校科研项目(2013A-069)

Single Nucleotide Polymorphisms in A-FABP Associated with Carcass Quality and Meat Quality Traits in Five Zaosheng Cattle Groups

Tang Hongjie Zhao Shengguo Lei Zhaomin Wang Xinrong Wang Jianfu Cai Yuan Wu Jianping

(Faculty of Animal Sci-Tech，Gansu Agricultural University，Lanzhou 730070)

Received:2013-10-17 Published:2014-05-23 Online:2014-05-24

摘要/Abstract

摘要： 研究早胜牛类群A-FABP基因多态性及其与胴体品质和肉质性状的相关性。采用PCR-SSCP方法对5个早胜牛类群(庆阳类群、平凉类群、南德温与庆阳类群杂种、西门塔尔与平凉类群杂种、秦川牛与平凉类群杂种)的A-FABP基因进行多态性分析，分析基因型与胴体品质和肉质性状的相关性。结果显示，A-FABP基因第三外显子区存在c.408G>C的突变，并检测到3种基因型GG、GC和CC。胴体性状相关分析表明，GG基因型的胴体重显著低于CC基因型(P<0.05)；GG基因型屠宰率极显著低于GC基因型(P<0.01)，显著低于CC基因型(P<0.05)；GG基因型净肉率显著低于GC基因型(P<0.05)；GG基因型的眼肌面积显著低于GC和CC基因型(P<0.05)。肉质性状相关分析表明，GG基因型失水率显著高于CC基因型个体(P<0.05)；GG基因型剪切力极显著高于GC和CC基因型(P<0.01)；GG基因型蒸煮损失和pH均显著高于GC基因型(P<0.05)，极显著高于CC基因型(P<0.01)。A-FABP基因突变位点可作为胴体性状和肉质性状遗传标记。

关键词: 早胜牛, A-FABP基因, SNPs, 胴体品质, 肉质性状

Abstract: It was to study the polymorphism of A-FABP gene and its correlation with carcass quality and meat quality traits in Zaosheng cattle groups. Using PCR-SSCP to analyze the polymorphism of A-FABP gene and its correlation with carcass quality and meat quality traits in five Zaosheng cattle groups(Qingyang group, QZ and its hybrid with South Devon group, QZ×N；Pingliang group, PZ and its hybrid with Qinchuan cattle group, PZ×Q；PZ and its hybrid with Simmental group, PZ×S). Result showed that there has a base mutation at c.408G>C of A-FABP gene exon 3 and exist three genotypes GG, GC and CC. Statistical analysis of carcass traits shows that carcass weight with GG genotype was significantly lower than CC genotype(P<0.05)；dressing percentage with GG genotype was significantly lower than GC genotype(P<0.01), and significantly lower than CC genotype(P<0.05)；net meat percentage with GG genotype was significantly lower than GC genotype(P<0.05)；loin muscle area with GG genotype was significantly lower than GC and CC genotypes(P<0.05). Statistical analysis of meat quality traits showed that pressing loss with GG genotype was significantly higher than CC genotype(P<0.05)；shear force with GG genotype was significantly higher than GC and CC genotype(P<0.01)；cooking loss and pH with GG genotype was significantly higher than GC genotype(P<0.05), and significantly higher than CC genotype(P<0.01). This mutation of A-FABP gene could be considered as a locus to carcass and meat quality.

Key words: Zaosheng Cattle, A-FABP Gene, SNPs, Carcass quality, Neat quality traits

唐红杰, 赵生国, 雷赵民, 王欣荣, 王建福, 蔡原, 吴建平. 早胜牛类群A-FABP基因SNPs及其与胴体品质和肉质性状的相关性分析[J]. 生物技术通报, 2014, 0(5): 96-101.

Tang Hongjie, Zhao Shengguo, Lei Zhaomin, Wang Xinrong, Wang Jianfu, Cai Yuan, Wu Jianping. Single Nucleotide Polymorphisms in A-FABP Associated with Carcass Quality and Meat Quality Traits in Five Zaosheng Cattle Groups[J]. Biotechnology Bulletin, 2014, 0(5): 96-101.

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