天祝白牦牛IGF-2基因的cDNA克隆

生物技术通报 ›› 2014, Vol. 0 ›› Issue (5): 102-106.

天祝白牦牛IGF-2基因的cDNA克隆

李亚兰¹ 张全伟¹ 王雪莹¹ 马友记² 张勇¹ 赵兴绪¹

(1.甘肃农业大学动物医学院，兰州 730070；2.甘肃农业大学动物科技学院，兰州 730070)

收稿日期:2013-11-04 出版日期:2014-05-23 发布日期:2014-05-24
作者简介:李亚兰，女，硕士研究生，研究方向：临床兽医；E-mail：liyalan0819@163.com
基金资助:
"十二五"农村领域国家科技计划(2013AA102505-3)

Cloning of IGF-2 Gene cDNA in Tianzhu White Yak

Li Yalan¹ Zhang Quanwei¹ Wang Xueying¹ Ma Youji² Zhang Yong¹ Zhao Xingxu¹

(1. College of Veterinary Medicine，Gansu Agriculture University，Lanzhou 730070；2. College of Animal Science and Technology，Gansu Agriculture University，Lanzhou 730070)

Received:2013-11-04 Published:2014-05-23 Online:2014-05-24

摘要/Abstract

摘要： 旨在克隆天祝白牦牛胰岛素样生长因子2(IGF-2)基因编码区全长cDNA序列，为研究该基因的生理功能奠定基础。运用cDNA末端快速扩增(RACE)技术获得天祝白牦牛IGF-2基因全长cDNA序列。扩增获得天祝白牦牛IGF-2基因全长cDNA序列为1 060 bp(GenBank登录号：KF682139)，ORF长540 bp，编码179个氨基酸。其编码的氨基酸与已报道哺乳动物IGF-2氨基酸序列同源性在80%-92%之间。天祝白牦牛IGF-2基因的成功克隆为进一步研究该基因的功能奠定了基础。

关键词: 天祝白牦牛, 胰岛素生长因子2, cDNA末端快速扩增, 基因克隆, RT-PCR

Abstract: It was to clone insulin-like growth factor2(IGF-2)full length cDNA in Tianzhu white Yak, thus provide a theoretical basis tostudyits mechanisms. IGF-2 gene full length cDNA were cloned by rapid amplification of cDNA ends(RACE). The result showed that the full length of IGF-2 cDNA was 1 060 bp(Gene Bank accession No：KF682139), with an ORF 540 bp in length, which encoded 179 amino acids. The amino sequence analysis indicated that IGF-2 gene in Tianzhou white yak is highly homologous in most mammals. The full length cDNA of 1 060 bp IGF-2 gene was successfully cloned, which laid a basis for further studying of its roles in whit yak.

Key words: Tianzhu white yak, IGF-2 Gene, Rapid amplification of CDNA ends(RACE), Gene Clone, RT-PCR

李亚兰, 张全伟, 王雪莹, 马友记, 张勇, 赵兴绪. 天祝白牦牛IGF-2基因的cDNA克隆[J]. 生物技术通报, 2014, 0(5): 102-106.

Li Yalan, Zhang Quanwei, Wang Xueying, Ma Youji, Zhang Yong, Zhao Xingxu. Cloning of IGF-2 Gene cDNA in Tianzhu White Yak[J]. Biotechnology Bulletin, 2014, 0(5): 102-106.

参考文献

[1] Le Roith D. The insulin -like growth factor system[J]. Exp Diabesity Res, 2003, 4：205-212.
[2] Werner H, Adamo M, Robert CT, et al. Molecular and cellular aspects of insulin-like growth factor action[J].Vitamins and Hormones, 1994, 48：1-58.
[3] Rinderknecht E, Humbel RE. Amino-terminal sequences of two polypeptides from human serum with nonsuppressible insulin-like and cell-growth-promoting activities：evidence for structural homology with insulin B chain[J]. Proc Natl Acad Sci USA, 1976, 73(12)：4379-4381.
[4] 蒋思文, 彭健, 熊远著.胰岛素样生长因子2 研究进展[J].遗传, 2004, 26(2)：271-273.
[5] Lee MH, Jeon YJ, Lee SM, et al. Placental gene expression is related to glucose metabolism and fetal cord blood levels of insulin and insulin-like growth factors in intrauterine growth restriction[J].Early Hum Dev, 2010, 86(1)：45-50.
[6] Aslan O, Hamill RM, Davey G, et al. Variation in the IGF2 gene promoter region is associated with intramuscular fatcontentin porcine skeletal muscle[J]. Mol Biol Rep, 2012, 39(4)：4101-4110.
[7] Geisert RD, Chamberlain CS, Vonnahme KA, et al. Possible role of kallikrein in proteolysis ofinsulin-like growth factor binding proteins during the oestrous cycle and early pregnancy in pigs[J].Reproduction, 2001, 121：719-728.
[8] 王丁科, 阎萍, 梁春年, 等. 牦牛IGF2内含子的遗传多态性及其遗传效应分析[J].华北农学报, 2009, 24(2)：107-111.
[9] MAFFT, version 7.113, Computational Biology Research Center(CBRC)：Tokyo, Japan, 2013.
[10] 王丁科, 阎萍, 梁春年, 等. 胰岛素样生长因子2研究进展[J].动物医学进展, 2008, 29(7)：67-70.
[11] Surani MA. Reprogramming of genome function through epigenetic inheritance[J].Nature, 2001, 414：122-128.
[12] Dindot SV, Kent KC, Evers B, et al. Conservation of genomic imprinting at the XIST, IGF2 and GTL2 loci in the bovine[J].Incorporating Mouse Genome Mammalian Genome Genes and Phenotypes, 2004, 15(12)：966-974.
[13] Hu Q, Ma T, Wang K, et al. The Yak genome database：an integrative database for studying yak biology and high-altitude adaption[J]. BMC Genomics, 2012, 13：600.
[14] Jones JI, Clemmons DR. Insulin-like growth factors and their binding proteins：biological actions[J]. Endocrine Reviews , 1995, 16：3-34.
[15] Sandra D, O'Dell, Ian NM Day. Molecules in focus Insulin-like growth factor II(IGF-II)[J].The International Journal of Biochemistry & Cell Biology, 1998, 30：767-771.
[16] Long RJ, Apori SO, Castro FB, ?rskov ER, et al. Feed value of native forages of the Tibetan Plateau of China[J].Animal Feed Science and Technology, 1999, 80(2)：101-113.
[17] Fazio S, Palmieri EA, Biondi B, et al. The role of the GH-IGF-I axis in the regulation of myocardial growth：from experimental models to human evidence[J]. European Journal of Endocrinology, 2000, 142：211-216.