激发子对桦褐孔菌多酚合成的诱导及其生化机制初探

生物技术通报 ›› 2014, Vol. 0 ›› Issue (9): 136-141.

激发子对桦褐孔菌多酚合成的诱导及其生化机制初探

魏志文¹,孙勇¹,王菲²

1.江苏师范大学江苏省药用植物生物技术重点实验室，徐州 221116;
2.江苏师范大学生命科学学院，徐州 221116

收稿日期:2014-04-23 出版日期:2014-09-15 发布日期:2014-09-07
作者简介:魏志文，男，硕士，研究方向：应用微生物；E-mail: zhiwen_wei@126.com
基金资助:
江苏师范大学自然科学基金项目（13XLB02）

The Production of Phenolic Compounds of Inonotus obliquus by Fungal Elicitor Introducing and Its Study of Biochemical Mechanism

Wei Zhiwen¹,Sun Yong¹, Wang Fei²

1. Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province，Jiangsu Normal University，Xuzhou 221116;
2. School of Life Science，Jiangsu Normal University，Xuzhou 221116

Received:2014-04-23 Published:2014-09-15 Online:2014-09-07

摘要/Abstract

摘要： 旨在研究真菌激发子对桦褐孔菌多酚合成的影响，采用液体摇瓶培养方式，将真菌激发子加入到桦褐孔菌液体培养物中，检测桦褐孔菌次级代谢产物的积累，以及真菌激发子对桦褐孔菌一氧化氮合酶（NOS）活性的影响。结果表明，适量添加真菌激发子能够提高桦褐孔菌的代谢速率，提高多酚类化合物的合成，胞内和胞外多酚最高可达102.15mg/L和311.91mg/L，并能显著提高桦褐孔菌NOS的活性，其活性最高可达151.93U/mgprot，发酵液一氧化氮（NO）含量最高可达256.28μmol/L。这表明，真菌激发子能够有效激活桦褐孔菌的次级代谢产物合成途径。

关键词: 真菌激发子, 酚类化合物, 桦褐孔菌, 次级代谢产物, 深层发酵

Abstract: In order to study the effects of fungal elicitor on secondary metabolites of Inonotus obliquus, using shaking flask method, the fungal elicitor was added to the liquid cultures of Inonotus obliquus, detected the accumulation of secondary metabolites, and the effects of fungal elicitor on Inonotus obliquus nitric oxide synthase（NOS）activity, discussed the influence of elicitors to Inonotus obliquus liquid fermentation process and the accumulation of secondary metabolites. The results showed that the addition of fungal elicitor could improve the metabolic rate of Inonotus obliquus and the accumulation of polyphenols. The intracellular and extracellular polyphenols were up to 102.15 mg/L and 311.91 mg/L, and could also significantly improve the activity of NOS, NOS activity was up to 151.93 U/mg prot, nitric oxide content in the fermentation liquid was up to 256.28 μmol/L. This showed, fungal elicitor could effectively active the biosynthesis pathway of secondary metabolites of Inonotus obliquus.

魏志文,孙勇,王菲. 激发子对桦褐孔菌多酚合成的诱导及其生化机制初探[J]. 生物技术通报, 2014, 0(9): 136-141.

Wei Zhiwen,Sun Yong, Wang Fei. The Production of Phenolic Compounds of Inonotus obliquus by Fungal Elicitor Introducing and Its Study of Biochemical Mechanism[J]. Biotechnology Bulletin, 2014, 0(9): 136-141.

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