基因组挖掘技术及其在真菌中的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2019-0170

摘要/Abstract

摘要： 微生物次级代谢物是药物的重要来源，在当前以活性为导向的传统筛选机制出现停滞时如何采用新的筛选方法减少已知化合物的重复检测，快速得到结构新颖的有特殊生物活性的新化合物是药物开发的关键。基因组挖掘技术可控制编码次级代谢产物基因的定向表达，将科学理论与工业化生产相结合并对其进行指导，有效降低开发成本及周期，有利于最终的市场应用而成为国内外研究的热点。简述了基因组挖掘技术的研究方向和激活沉默基因表达的技术手段，以及真菌在基因组挖掘中的重要地位和最近几年的研究成果，并展望了未来在医药领域的广阔前景。

关键词: 次级代谢产物, 基因组挖掘, 沉默基因激活

Abstract: Microbial secondary metabolites are important sources of drugs. When the traditional activity-oriented screening mechanism is stagnant，how to adopt new screening methods to reduce the repeated detection of known compounds and to quickly obtain new compounds with novel structures and special biological activities is the key to drug development. Genomic mining technology has become a research hotspot at home and abroad because it may control the directed expression of genes encoding secondary metabolites，combine scientific theories with industrial production and guide them，effectively reduce the development cost and cycle，and contribute to the final market application. In this paper，the research direction of genome mining technology and the technical means of activating silenced gene expression are summarized，the important status of fungi in genome mining and the research achievements in recent years are also described，and the broad prospect of fungi in the field of medicine is highlighted.

Key words: secondary metabolites, genome mining, activation of silenced gene

徐杰, 黄建忠, 李力. 基因组挖掘技术及其在真菌中的研究进展[J]. 生物技术通报, 2019, 35(11): 201-207.

XU Jie ,HUANG Jian-zhong, LI Li. Summary of Genomics Mining Technology and Its Research Progress in Fungi[J]. Biotechnology Bulletin, 2019, 35(11): 201-207.

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