膜蛋白原核表达的基因序列优化

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.007

摘要/Abstract

摘要： 膜蛋白的结构与功能研究是当前的热点之一。目前获取膜蛋白的最主要途径是通过原核系统进行表达。但是膜蛋白在原核系统中表达水平相对较低，通过对基因序列进行优化促使膜蛋白正确高效表达是一项非常重要的技术手段。归纳了近些年来膜蛋白原核表达技术在基因序列优化研究上的最新进展，并从稀有密码子的优化、mRNA的稳定性与翻译起始、mRNA与核糖体行为、翻译的效率与膜蛋白的折叠4个方面对基因序列上影响膜蛋白表达的因素进行了总结，旨在为膜蛋白的原核表达提供一定的思路和参考。

关键词: 膜蛋白, 超量表达, 基因序列优化, 密码子优化, mRNA稳定性, 翻译速率, 膜蛋白折叠

Abstract: Studies of the structure and function of membrane proteins are hotspots of life science in recent years. The most important approach to acquire membrane protein is recombinant expression in prokaryotic system. However, the yield of membrane protein in prokaryotic system is relatively low. Gene sequence optimization is a effective technique to enhance expression of membrane protein in prokaryotic system. In this article, we summarize the latest progress on gene sequence optimization for the prokaryotic expression of membrane proteins, and discuss the factors which affect the expression of membrane protein, including the optimization of rare codons, mRNA stability and translation starting, mRNA and ribosome behaviour, translation rate and membrane protein folding. Our paper provides new possibilities for the over-expression of membrane protein in prokaryotic system.

Key words: membrane protein, over-expression, gene sequence optimization, rare codons optimization, mRNA stability, translation rate, membrane protein folding

王世山,陈艳可,杨俊. 膜蛋白原核表达的基因序列优化[J]. 生物技术通报, 2015, 31(12): 50-55.

Wang Shishan, Chen Yanke, Yang Jun. The Gene Sequence Optimization of Membrane Protein in Prokaryotic Expression System[J]. Biotechnology Bulletin, 2015, 31(12): 50-55.

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