转基因水稻cry1Ab/Ac基因及蛋白在肉鸡肠道中的残留检测

doi:10.13560/j.cnki.biotech.bull.1985.2015.09.011

生物技术通报 ›› 2015, Vol. 31 ›› Issue (9): 84-90.doi: 10.13560/j.cnki.biotech.bull.1985.2015.09.011

转基因水稻cry1Ab/Ac基因及蛋白在肉鸡肠道中的残留检测

徐灯^1，2, 耿丽丽², 张敏红³, 卢凡¹, 张杰²

(1. 湖北工业大学资源与环境学院，武汉 430068；2. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室，北京 100193；3. 中国农业科学院北京畜牧兽医研究所，北京 100193)

收稿日期:2015-01-28 出版日期:2015-09-15 发布日期:2015-09-16
作者简介:徐灯，男，硕士，研究方向：转基因生物安全评价；E-mail：xudg1230@163.com
基金资助:
转基因生物新品种培育重大专项(2012ZX08011001-005)

Detection of cry1Ab/Ac Gene and Protein Remained in Gut of Broiler Feeding Transgenic Rice

Xu Deng^1，2, Geng Lili², Zhang Minhong³, Lu Fan¹, Zhang Jie²

(1. School of Resource & Environmental Engineering，Hubei University of Technology，Wuhan 430068；2. State Key Laboratory for Biology of Plant Diseases and Insect Pests，Institute of Plant Protection，Chinese Academy of Agricultural Sciences，Beijing 100193；3. Institute of Animal Science，Chinese Academy of Agricultural Sciences，Beijing 100193)

Received:2015-01-28 Published:2015-09-15 Online:2015-09-16

摘要/Abstract

摘要： 旨在研究转基因水稻对饲喂肉鸡的影响，以含有转cry1Ab/Ac基因水稻的饲料饲喂肉鸡，分析了肉鸡空肠、回肠、盲肠、直肠中cry1Ab/Ac基因和蛋白残留情况，并用体外实验验证了外源基因的降解情况。结果表明，饲喂转cry1Ab/Ac基因水稻21 d和42 d的肉鸡肠道4个部位中没有检测到cry1Ab/Ac基因残留，体外消化外源基因的实验发现外源基因在肉鸡肠道中约4 h后开始逐步降解。对上述4个部位内容物进行ELISA和Western blot分析，均未检测到 Cry1Ab/Ac蛋白残留。综合分析表明，转基因水稻中的外源基因和蛋白在肉鸡肠道中易被消化和降解。

关键词: 转基因水稻, 肉鸡, 基因及蛋白残留, 体外消化

Abstract: To analyze the effects of genetically modified rice on feeding broilers, cry1Ab/Ac gene and protein remained in the jejunum, ileum, cecum and rectum of broiler feeding transgenic rice were detected, and the degradation patterns were testified in vitro. The results showed that there were no residues of cry1Ab/Ac gene in 4 intestinal parts of broilers fed transgenic rice for 21 and 42 d, and cry1Ab/Ac gene gradually degraded after 4 h in the gut in vitro test. No residues of Cry1Ab/Ac protein were detected in contents of the above 4 parts of broilers using ELISA and Western blot. In conclusion, cry1Ab/Ac gene and protein of the transgenic rice were easily digested and degraded in broiler intestines.

Key words: transgenic rice, broiler, gene and protein residues, digestion in vitro

徐灯, 耿丽丽, 张敏红, 卢凡, 张杰. 转基因水稻cry1Ab/Ac基因及蛋白在肉鸡肠道中的残留检测[J]. 生物技术通报, 2015, 31(9): 84-90.

Xu Deng, Geng Lili, Zhang Minhong, Lu Fan, Zhang Jie. Detection of cry1Ab/Ac Gene and Protein Remained in Gut of Broiler Feeding Transgenic Rice[J]. Biotechnology Bulletin, 2015, 31(9): 84-90.

参考文献

[1]Tu JM, Zhang GA, Datta K, et al. Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis delta-endotoxin[J]. Nature Biotechnology, 2000, 18(10)：1101-1104.
[2]Lu C. The first approved transgenic rice in China[J]. GM Crops, 2010, 1(3)：113-115.
[3]James C. Global Status of Commercialized Biotech/GM Crops：2013[R]. ISAAA Brief 46, 2014.
[4]许文涛, 贺晓云, 黄昆仑, 等. 转基因植物的食品安全性问题及评价策略[J]. 生命科学, 2011, 23(2)：179-185.
[5]Swiatkiewicz S, Koreleski J, Arczewska-Wlosek A, et al. Detection of transgenic dna from Bt maize and herbicide tolerant soybean meal in tissues, eggs and digestive tract content of laying hens fed diets containing[J]. Annals of Animal Science, 2011, 11(3)：413-424.
[6]Jennings JC, Albee LD, Kolwyck DC, et al. Attempts to detect transgenic and endogenous plant DNA and transgenic protein in muscle from broilers fed Yield Gard Corn Borer Corn[J]. Poultry Science, 2003, 82(3)：371-380.
[7] Ash J, Novak C, Scheideler SE. The fate of genetically modified protein from Roundup Ready Soybeans in laying hens[J]. Journal of Applied Poultry Research, 2003, 12(2)：242-245.
[8]杜红方. 转基因水稻作为肉仔鸡日粮原料的安全性评价[D]. 北京：中国农业科学院, 2006.
[9]秦海峰. 转 Cry1Ac/sck 基因糙米作为肉仔鸡日粮原料的安全性评价[D]. 北京：中国农业科学院, 2012.
[10]Ding JY, Jia JW, Yang LT, et al. Validation of a rice specific gene, sucrose phosphate synthase, used as the endogenous reference gene for qualitative and real-time quantitative PCR detection of transgenes[J]. Journal of Agricultural and Food Chemistry, 2004, 52(11)：3372-3377.
[11]Jeong SC, Pack IS, Cho EY, et al. Molecular analysis and quantitative detection of a transgenic rice line expressing a bifunctional fusion TPSP[J]. Food Control, 2007, 18(11)：1434-1442.
[12]Bannon GA. What makes a food protein an allergen?[J]. Current Allergy and Asthma Reports, 2004, 4(1)：43-46.
[13]Rossi F, Morlacchini M, Fusconi G, et al. Effect of Bt corn on broiler growth performance and fate of feed-derived DNA in the digestive tract[J]. Poultry Science, 2005, 84(7)：1022-1030.
[14]Tony MA, Butschke A, Broll H, et al. Safety assessment of Bt 176 maize in broiler nutrition：Degradation of maize-DNA and its metabolic fate[J]. Archives of Animal Nutrition-Archiv Fur Tierernahrung, 2003, 57(4)：235-252.
[15]Chowdhury EH, Shimada N, Murata H, et al. Detection of Cry1Ab protein in gastrointestinal contents but not visceral organs of genetically modified Bt11-fed calves[J]. Vet Hum Toxicol, 2003, 45(2)：72-75.
[16]Einspanier R, Lutz B, Rief S, et al. Tracing residual recombinant feed molecules during digestion and rumen bacterial diversity in cattle fed transgene maize[J]. European Food Research and Technology, 2004, 218(3)：269-273.
[17]Lutz B, Wiedemann S, Einspanier R, et al. Degradation of Cry1Ab protein from genetically modified maize in the bovine gastrointestinal tract[J]. J Agric Food Chem, 2005, 53(5)：1453-1456.
[18]Ma QG, Gao CQ, Zhang JY, et al. Detection of transgenic and endogenous plant DNA fragments and proteins in the digesta, blood, tissues, and eggs of laying hens fed with phytase transgenic corn[J]. PLoS One, 2013, 8(4)：10.
[19]Gao CQ, Ma QG, Zhao LH, et al. Effect of dietary phytase transgenic corn on physiological characteristics and the fate of recombinant plant DNA in laying hens[J]. Asian-Australasian Journal of Animal Sciences, 2014, 27(1)：77-82.
[20]Stadnik J, Karwowska M, Dolatowski ZJ, et al. Effect of genetically modified, insect resistant corn(Mon 810)and glyphosate tolerant soybean meal(Roundup Ready)on physico-chemical properties of broilers’ breast and thigh muscles[J]. Bulletin of the Veterinary Institute in Pulawy, 2011, 55(3)：541-546.
[21]Sieradzki Z, Mazur M, Kwiatek K, et al. Assessing the possibility of genetically modified DNA transfer from GM feed to broiler, laying hen, pig and calf tissues[J]. Polish Journal of Veterinary Sciences, 2013, 16(3)：435-441.

转基因水稻cry1Ab/Ac基因及蛋白在肉鸡肠道中的残留检测

Detection of cry1Ab/Ac Gene and Protein Remained in Gut of Broiler Feeding Transgenic Rice

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