蛹虫草基质多糖对鸡传染性支气管炎灭活疫苗的免疫佐剂作用

生物技术通报 ›› 2016, Vol. 32 ›› Issue (12): 108-112.

蛹虫草基质多糖对鸡传染性支气管炎灭活疫苗的免疫佐剂作用

周梅仙,周业飞

南京晓庄学院食品科学学院，南京 211171

收稿日期:2016-04-27 出版日期:2016-12-25 发布日期:2016-12-07
作者简介:周梅仙，副教授，研究方向：动物养殖；E-mail：492773339@qq.com
基金资助:
南京晓庄学院院级重点项目（2013NXY10）

Adjuvanticity of Cordyceps militaris stroma Polysaccharides in Inactivated Vaccine to Avian Infectious Bronchitis

ZHOU Mei-xian,ZHOU Ye-fei

Department of Food Science，Nanjing Xiaozhuang University，Nanjing 211171

Received:2016-04-27 Published:2016-12-25 Online:2016-12-07

摘要/Abstract

摘要： 以蛹虫草基质多糖为免疫佐剂，将其混入传染性支气管炎灭活疫苗，从而探讨其对肉仔鸡的免疫功能影响。选用150只1日龄黄羽肉鸡，随机分成5组。免疫后21 d（35日龄）采用IBV M41株病毒液进行点眼、滴鼻攻毒。通过测定每组的鸡淋巴细胞（PBMC）增殖情况、鸡血清抗体效价、IBV强毒株攻毒保护及组织病理学变化。结果表明，蛹虫草基质多糖中剂量佐剂组的鸡淋巴细胞（PBMC）增殖指数、鸡血清抗体效价水平有着显著提高（P<0.05），在IBV M41株攻毒试验中，蛹虫草基质多糖中剂量佐剂组可以显著减轻病毒感染肉仔鸡临床症状，肺和肾无组织病理学变化，免疫保护率达到96.7%。表明以蛹虫草基质多糖佐剂能够提高肉仔鸡对传染性支气管炎的免疫力。

关键词: 蛹虫草基质多糖, 鸡传染性支气管炎, 免疫佐剂

Abstract: It aimed to use Cordyceps militaris stroma polysaccharides as adjuvants that mix with inactivated vaccine to infectious bronchitis，and to investigate its effects on the immune functions of broilers. Total 150 1-day Huangyu broilers were selected and randomly divided into 5 groups. Then the broilers of 21 d after immunized（35-day）were infected with Mass 41 infectious bronchitis virus（IBV M41）strain by the ocular-nasal route. The proliferation of peripheral blood mononuclear cells（PBMC），serum antibody titer，the protection of IBV virulent strain，and histopathological changes in each group were evaluated respectively by MTT method and hemagglutination inhibition assay. The results showed that proliferation index of PBMC and serum antibody titer in the broilers with dosage C. militaris stroma polysaccharides as adjuvants significantly raised（P<0.05）. Following challenge test with IBV M41 strain，broilers inoculated with C. militaris stroma polysaccharides showed significantly lighter clinical symptoms，there were no histopathological changes in lung and kidney，and immune protection reached 96.7%. This indicated that C. militaris stroma polysaccharides as adjuvants increased the immunity of broilers to infectious bronchitis.

Key words: Cordyceps militaris stroma polysaccharides, avian infectious bronchitis, adjuvants

中图分类号:

10.13560/j.cnki.biotech.bull.1985.2016.12.018

周梅仙,周业飞. 蛹虫草基质多糖对鸡传染性支气管炎灭活疫苗的免疫佐剂作用[J]. 生物技术通报, 2016, 32(12): 108-112.

ZHOU Mei-xian,ZHOU Ye-fei. Adjuvanticity of Cordyceps militaris stroma Polysaccharides in Inactivated Vaccine to Avian Infectious Bronchitis[J]. Biotechnology Bulletin, 2016, 32(12): 108-112.

参考文献

[1] Abd El Rahman S, Winter C, El-Kenawy A, et al. Differential sensitivity of well-differentiated avian respiratory epithelial cells to infection by different strains of infectious bronchitis virus[J] . Journal of Virology, 2010, 84：8949-8952.
[2] Autran B, Carcelain G, Combadiere B, et al. Therapeutic vaccines for chronic infections[J] . Science, 2004, 305：205-208.
[3] Aghasadeghi MR, Salmani AS, Sadat SM, et al. Application of outer membrane vesicle of Neisseria meningitidis serogroup B as a new adjuvant to induce strongly Th1-oriented responses against HIV-1[J] . Current HIV Research, 2011, 9：630-635.
[4] Degen WG, van Zuilekom HI, Scholtes NC, et al. Potentiation of humoral immune responses to vaccine antigens by recombinant chicken IL-18（rChIL-18）[J] . Vaccine, 2005, 23：4212-4218.
[5] Lee S, Lee HH, Kim J, et al. Anti-tumor effect of Cordyceps militaris in HCV-infected human hepatocarcinoma 7. 5 cells[J] . The Journal of Microbiology, 2015, 53（7）：468-474.
[6] Lee HH, Lee S, Lee K, et al. Anti-cancer effect of Cordyceps militaris in human colorectal carcinoma RKO cells via cell cycle arrest and mitochondrial apoptosis[J] . DARU Journal of Pharmaceutical Sciences, 2015, 23：35.
[7] Huang SJ, Lin CP, Mau JL, et al. Effect of UV-B Irradiation on Phys-iologically active substance content and antioxidant properties of the medicinal caterpillar fungus Cordyceps militaris （Ascomycetes）[J] . Int J Med Mushrooms, 2015, 17（3）：241-253.
[8] 江海涛, 吴雨龙, 等. 蛹虫草基质多糖对酒精所致小鼠急性肝损伤的保护作用[J] . 食品科学, 2014, 35（13）：223-227.
[9] 中国兽药典委员会. 中华人民共和国兽药典[M] . 北京：中国农业出版社, 2010.
[10] Jiang W, Jiang P, Li Y, et al. Recombinant adenovirus expressing GP5 and M fusion proteins of porcine reproductive and respiratory syndrome virus induce both humoral and cell-mediated immune responses in mice[J] . Veterinaryimmunology and Immunopathology, 2006, 113（1-2）：169-180.
[11] Office International des Epizooties（O. I. E.）, Manual of standards for diagnostic tests and vaccines[S] . Avian Infectious Bronchitis, 6th edn, 2008：443-455.
[12] Jawale CV, Lee JH. Characterization of a Salmonella Typhimurium ghost carrying an adjuvant protein as a vaccine candidate for the protection of chickens against virulent challenge[J] . Avian Pathology, 2014, 43（6）：506-513.
[13] Ding W, Guo X, Ataku K. Characterization of peptides in ensiled alfalfa treated with different chemical additives[J] . Animal Science Journal, 2013, 84（12）：774-781.
[14] Mosmann TR, et al. The expanding universe of T-cell subsets：Th1, Th2 and more[J] . Immunol Today, 1996, 17（3）：138-146.
[15] Meir R, Krispel S, Simanov L, et al. Immune responses to mucosal vaccination by the recombinant A1 and N proteins of infectious bronchitis virus[J] . Viral Immunology, 2012, 25：55-62.
[16] Lyu K, Wang GC, He YL, et al. Crystal structures of enterovirus 71（EV71）recombinant virus particles provide insights into vaccine design[J] . J Biol Chem, 2015, 290（6）：3198-3208.
[17] Ju XH, Xu HJ, Yong YH, et al. Heat stress upregulation of Toll-like receptors 2/4 and acute inflammatory cytokines in peripheral blood mononuclear cell（PBMC）of Bama miniature pigs：an in vivo and in vitro study[J] . Animal, 2014, 8（9）：1462-1468.