山楂提取物对急性损伤线虫的保护作用

doi:10.13560/j.cnki.biotech.bull.1985.2016.04.035

生物技术通报 ›› 2016, Vol. 32 ›› Issue (4): 256-260.doi: 10.13560/j.cnki.biotech.bull.1985.2016.04.035

山楂提取物对急性损伤线虫的保护作用

赵江¹,陈纯¹,王轶菲¹，²,王红¹，²,黄杰¹，²,王浩¹

1. 天津科技大学食品工程与生物技术学院，天津 300457;
2. 天津科技大学生物工程学院，天津 300457

收稿日期:2015-08-31 出版日期:2016-04-25 发布日期:2016-04-26
作者简介:赵江，男，硕士研究生，研究方向：食品营养学研究；E-mail：tozjmail@163.com
基金资助:
“十二五”国家科技计划项目（2012BAD33B05）

The Protective Effect of Haworth Fruit Extract on Caenorhabditis elegans in Acute Damages

ZHAO Jiang¹, CHEN Chun¹, WANG Yi-fei¹，² ,WANG Hong¹，² ,HUANG Jie¹，² ,WANG Hao¹

1. College of Food Engineering and Biotechnology，Tianjin University of Science and Technology，Tianjin 300457;
2. School of Biological Engineering，Tianjin University of Science & Technology，Tianjin 300457

Received:2015-08-31 Published:2016-04-25 Online:2016-04-26

摘要/Abstract

摘要： 以秀丽隐杆线虫（Canorhabditis elegans）为模式生物研究山楂提取物（Haworth fruit extract，HFE）对其急性氧化损伤的保护作用及其可能的作用机制。饲喂线虫于含有不同浓度（0、25、50和100 μg/mL）HFE的NGM（Nematode growth medium）培养基中，研究HFE对线虫急性应激耐受能力的影响。结果显示，饲喂HFE后，秀丽线虫表现出比正常组更高的寿命，并且在胡桃醌氧化应激、热应激及紫外辐射应激实验中寿命均明显延长，荧光显微镜观察发现HFE组线虫的脂褐素自发荧光明显减弱，并且与HFE浓度呈剂量依赖效应。 HFE能够显著延长秀丽隐杆线虫的寿命，同时对多种氧化损伤具有较好的保护作用，改善机体的抗氧化能力，有效延缓衰老。

关键词: 秀丽隐杆线虫, 山楂提取物, 急性损伤, 延缓衰老

Abstract: The work aims to study the protective effect of haworth fruit extract（HFE）on the acute oxidative damage and its possible protection mechanism in Caenorhabditis elegans. The C. elegans were fed with nematode growth medium（NGM）containing different dose of HFE（0，25，50 and 100 μg/mL）to study impacts of HFE on the acute stress tolerance. C. elegans with feeding in different dose of HFE had a longer lifespan than the normal group，moreover，their lifespans became significantly longer under the experiments of juglone oxidative stress，heat stress and UV radiation stress. Concurrently，the autofluorescence level of lipofuscin in HFE group of C. elegans significantly decreased and it was dose-dependent effect to the concentration of HFE. In conclusion，HFE significantly prolongs the lifespan of C. elegans and has a solid protection under a variety of oxidative stress conditions with improving the antioxidant capacity and efficient anti-aging

Key words: Caenorhabditis elegans, extract of haworth fruit, acute damage, anti-aging

赵江,陈纯,王轶菲,王红,黄杰,王浩. 山楂提取物对急性损伤线虫的保护作用[J]. 生物技术通报, 2016, 32(4): 256-260.

ZHAO Jiang, CHEN Chun, WANG Yi-fei， ,WANG Hong， ,HUANG Jie， ,WANG Hao. The Protective Effect of Haworth Fruit Extract on Caenorhabditis elegans in Acute Damages[J]. Biotechnology Bulletin, 2016, 32(4): 256-260.

参考文献

［1］Beal MF. Aging, energy, and oxidative stress in neurodegenerative diseases［J］. Annals of Neurology, 1995, 38（3）：357-366.
［2］ Niki E. Lipid peroxidation and its inhibition：overview and perspe-ctives［J］. Journal of Oleo Science, 2001, 50：313-320.
［3］ Escobedo J, Pucci AM, Koh TJ. HSP25 protects skeletal muscle cells against oxidative stress［J］. Free Radical Biology and Medicine, 2004, 37（9）：1455-1462.
［4］ Rand JB, Johnson CD. Genetic pharmacology：interactions between drugs and gene products in Caenorhabditis elegans［J］. Methods in Cell Biology, 1995, 48：187-204.
［5］Honda Y, Honda S. Oxidative stress and life span determination in the nematode Caenorhabditis elegans［J］. Annals of the New York Academy of Sciences, 2002, 959（1）：466-474.
［6］Lee EY, Shim YH, Chitwood DJ, et al. Cholesterol-producing transgenic Caenorhabditis elegans lives longer due to newly acquired enhanced stress resistance［J］. Biochemical and Biophysical Research Communications, 2005, 328（4）：929-936.
［7］Liu P, Yang B, Kallio H. Characterization of phenolic compounds in Chinese hawthorn（Crataegus pinnatifida Bge. var. major）fruit by high performance liquid chromatography-electrospray ionization mass spectrometry［J］. Food Chemistry, 2010, 121（4）：1188-1197.
［8］Von EM, Brunner H, Haegeli A, et al. Hawthorn/passion flower extract and improvement in physical exercise capacity of patients with dyspnoea class II of the NYHA functional classification［J］. Acta Therapeutica, 1994, 20：47-66.
［9］Liu T, Cao Y, Zhao M. Extraction optimization, purification and antioxidant activity of procyanidins from hawthorn（C. pinnatifida Bge. var. major）fruits［J］. Food Chemistry, 2010, 119（4）：1656-1662.
［10］汪晓燕, 王香明, 王丹巧, 等. 不同生长阶段线虫衰老相关基因的表达变化及川芎提取物的调控作用［J］. 中国中药杂志, 2010（12）：1599-1602.
［11］Abbas S, Wink M. Epigallocatechin gallate inhibits beta amyloid oligomerization in Caenorhabditis elegans and affects the daf-2/insulin-like signaling pathway［J］. Phytomedicine, 2010, 17（11）：902-909.
［12］Liu H, Liang F, Su W, et al. Lifespan extension by n-butanol extract from seed of Platycladus orientalis in Caenorhabditis elegans［J］. Journal of Ethnopharmacology, 2013, 147（2）：366-372.
［13］ de Castro E, et al. Isolation of long-lived mutants in Caenorhabditis elegans using selection for resistance to juglone［J］. Free Radical Biology and Medicine, 2004, 37（2）：139-145.
［14］陆婉, 曲中原, 邹翔, 等. 胡桃醌的研究进展［J］. 2008 年中国药学会学术年会暨第八届中国药师周论文集, 2008.
［15］Wiegant FAC, Surinova S, Ytsma E, et al. Plant adaptogens increase lifespan and stress resistance in C. elegans［J］. Biogerontology, 2009, 10（1）：27-42.
［16］Lithgow G J, Walker G A. Stress resistance as a determinate of C. elegans lifespan［J］. Mechanisms of Ageing and Development, 2002, 123（7）：765-771.
［17］Vayndorf EM, Lee SS, Liu RH. Whole apple extracts increase lifespan, healthspan and resistance to stress in Caenorhabditis elegans［J］. Journal of Functional Foods, 2013, 5（3）：1235-1243.

[1]	赵金玲, 安磊, 任晓亮. 单细胞转录组测序技术及其在秀丽隐杆线虫中的应用[J]. 生物技术通报, 2023, 39(6): 158-170.
[2]	安磊, 赵金玲, 任晓亮. RNA修饰及其在秀丽隐杆线虫中的研究进展[J]. 生物技术通报, 2023, 39(4): 176-186.
[3]	潘文娟, 林家富, 王小桃, 郭义东, 褚以文, 刘超兰. 西藏湖泊放线菌的分离鉴定及抗菌活性测定[J]. 生物技术通报, 2020, 36(7): 97-103.

山楂提取物对急性损伤线虫的保护作用

The Protective Effect of Haworth Fruit Extract on Caenorhabditis elegans in Acute Damages

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价