关键词: 沙冬青种子, 总黄酮, AlCl3显色法, 大孔树脂, 萃取, 纯化

Abstract: This work is to establish the determination and purification method of total flavonoids from seeds of Ammopiptanthus mongolica. Four chromogenic methods were chosen for the determination of total flavonoids,the best absorption wavelengths were selected by UV-wavelength scanning of quercetin standard and total flavonoids from seeds of A. mongolica. Standard curves for quercetin by four chromogenic methods were built,and each method was verified via methodology,and the new experimental condition and method after verification were used to determine the content of total flavonoids from seeds of A. mongolica. In this experiment,3 kinds of macroporous resins of AB-8,D101,and S-8 were selected for static adsorption and elution test,and AB-8 was chosen for dynamic test,with which the process condition for purifying total flavonoids from seeds of A. mongolica was established,and the extraction was conducted with ethyl acetate to improve the purity of total flavonoid. The results showed that the determination of total flavonoids from seeds of A. mongolica with AlCl₃-CH₄O chromogenic method at 335 nm and quercetin as the standard substance was reliable and effective. Under the optimal condition,adsorption with AB-8 macroporous resins was 5 BV of 6 mg/mL sample solution at pH 5.5 at rate of 3.5 BV/h,and then elution was 6 BV of 70% ethanol at rate of 1.5 BV/h,the purity of the product was 41.07% from 5.69% before purification. The total flavonoids after purified with AB-8 resin were prepared into a certain concentration solution,and then extracted with ethyl acetate,the total flavonoids content reached 76.15%.

Key words: seeds of Ammopiptanthus mongolica, total flavonoids, AlCl3 chromogenic method, macroporous resin, extraction, purification