红色荧光蛋白的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2017-0401

摘要/Abstract

摘要： 从荧光蛋白的首次发现至今,其不断丰富的荧光光谱以及日益增多的光化学特性促使其在生物学研究中的地位不断提升。1999年出现的红色荧光蛋白以其卓越的优势在生物学研究中占领了重要地位。主要介绍了从红色荧光蛋白发现至今日趋重要的原因,以及它的显著优势。另外,重讨论了目前几种常见的红色荧光蛋白的特性及其在生物学研究中存在的优势和不足之处,为今后红色荧光蛋白的研究提供一个选择。

关键词: 红色荧光蛋白, 远红外荧光蛋白, 荧光计时器, 大斯托克斯荧光蛋白, 光活化荧光蛋白

Abstract: Since the first discovery of fluorescent proteins,its ever-increasing fluorescence spectra and the increasing number of photochemical properties have led to an increasing position in biological research. The red fluorescent protein emerged in 1999 dominated the biology study with its superiority. This paper focuses on the growing importance of fluorescent protein discovery from now on,as well as the significant advantages of red fluorescent protein. In addition,we discuss the characteristics of several common red fluorescent proteins and their advantages and disadvantages in biology research,providing an alternative for the future research of red fluorescent protein.

Key words: red fluorescent protein, far-red fluorescent protein, fluorescent timers, LSSFP, PAFP

王飞,杨海涛,王泽方. 红色荧光蛋白的研究进展[J]. 生物技术通报, 2017, 33(9): 32-47.

WANG Fei,YANG Hai-tao,WANG Ze-fang. Research Progress on Red Fluorescent Protein[J]. Biotechnology Bulletin, 2017, 33(9): 32-47.

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