牦牛KISS-1基因的克隆及其在生殖轴的表达

doi:10.13560/j.cnki.biotech.bull.1985.2017-0968

摘要/Abstract

摘要： KISS-1在动物繁殖调控中具有重要作用，旨为探讨KISS-1基因在牦牛季节性繁殖中的调控作用。实验采集5头成年母牦牛和5头黄牛的下丘脑、垂体等组织，利用RT-PCR和qPCR技术研究其KISS-1基因序列及组织表达特性。结果表明，牦牛和黄牛KISS-1基因编码区长度为408 bp，编码135个氨基酸，比对分析发现牦牛和黄牛基因编码区存在7处碱基突变。牦牛KISS-1基因氨基酸序列与黄牛、藏山羊、绵羊、野猪、人和褐家鼠的同源性分别为97%、85%、65%、55%和51.5%。KISS-1基因mRNA在牦牛和黄牛的下丘脑、垂体、卵巢、输卵管及子宫中均有表达。在下丘脑和脑垂体的表达丰度高，但两物种间无显著性差异（P>0.05）。说明KISS-1基因在动物进化中比较保守，对牦牛季节性繁殖的调控作用有待进一步研究。

关键词: 牦牛, 黄牛, KISS-1, 序列分析, 组织表达

Abstract: KISS-1 plays a pivotal role in the regulation of animal reproduction，thus this study is aimed to investigate the role of KISS-1 gene in regulating seasonal reproduction of the yak. Samples of hypothalamus，pituitary etc. were collected from five adult female yaks and five yellow cattle. KISS-1 gene sequence and its tissue expression characteristics were studied by RT-pcr and qPCR techniques. The results showed that the coding region length of yak and yellow cattle KISS-1 gene was 408 bp，which encoded 135 amino acids，and there were 7 base mutations between these two species. The amino acid sequence identities of yak KISS-1 gene with yellow cattle，Tibetan goats，sheep，wild pig，human，and sewer rat were 97.1%，85%，65%，55%，and 51.5% respectively. Expression of KISS-1 gene was detected in hypothalamus，pituitary，ovary，fallopian tube，and uterus of yak and cattle. The expression levels in hypothalamus and pituitary were the greatest，but there was no significant difference between the two species（P > 0.05）. It is concluded that KISS-1 gene is conservative in animal evolution，and its regulation roles in seasonal reproduction of the yak need to be further studied.

Key words: yak, yellow cattle, KISS-1, sequence analysis, tissue expression

杨远潇, 字向东,. 牦牛KISS-1基因的克隆及其在生殖轴的表达[J]. 生物技术通报, 2018, 34(3): 142-149.

YANG Yuan-xiao, ZI Xiang-dong. Cloning of KISS-1 Gene in Yak and Its Expression in Reproductive Axis[J]. Biotechnology Bulletin, 2018, 34(3): 142-149.

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