生物技术通报 ›› 2018, Vol. 34 ›› Issue (8): 138-143.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0145

• 研究报告 • 上一篇    下一篇

不同“桑黄”类真菌水提取物的抗肿瘤活性研究

武晓林1,2, 王超儀1, 包海鹰1   

  1. 1. 吉林农业大学,长春 134300;
    2. 吉林农业科技学院,吉林 132000
  • 收稿日期:2018-02-10 出版日期:2018-08-26 发布日期:2018-09-04
  • 作者简介:武晓林,女,博士研究生,研究方向:菌物药;E-mail:wuxiaolin2003@163.com
  • 基金资助:
    国家自然科学基金项目(31270088),吉林省道地药材生产利用技术创新省级重点实验室

Antitumor Effect of Water Extracts from Fruiting Bodies of Different “Sanghuang”Fungi

WU Xiao-lin1,2, WANG Chao-yi1, BAO Hai-ying1   

  1. 1. Jilin Agriculture University,Changchun 134300;
    2. Jilin Agriculture Science and Technology College,Jilin 132000
  • Received:2018-02-10 Published:2018-08-26 Online:2018-09-04

摘要: 用H22荷瘤小鼠对6种“桑黄”类真菌的水提取物进行了抗肿瘤活性研究。结果表明,除鲍姆木层孔菌水提取物低剂量组外,粗毛纤孔菌、椭圆嗜蓝孢孔菌、山野木层孔菌的水提取物高、低剂量组以及火木层孔菌、鲍姆木孔菌、瓦尼木层孔菌的水提取物高剂量组均具有显著抑瘤作用,抑瘤率均大于40%,其中;椭圆嗜蓝孢孔菌水提取物高剂量组(1 000 mg/kg)的抑瘤效果最佳。与阳性组和生理盐水组相比,粗毛纤孔菌高剂量组小鼠的胸腺指数均有极显著性差异(P<0.01),其余各组小鼠胸腺指数无显著性差异。6种“桑黄”类真菌水提取物均对提高小鼠肿瘤细胞中Bax的表达起到了促进作用;对Bcl-2的表达起到了抑制作用。

关键词: 桑黄, 粗毛纤孔菌, 火木层孔菌, 椭圆嗜蓝孢孔菌, 抗肿瘤

Abstract: The antitumor activities of water extracts from 6 “Sanghuang” fungi were studied with H22 mice in vivo. The results showed that the high and low dose groups of the water extracts from Inonotus hispidus,Fomitiporia ellipsoidea,and Phellinus yamanoi and high dose group of the water extracts from Phellinus igniarius,Phellinus baumii,and Phellinus vaninii presented significant antitumor effect with over 40% of antitumor rate,except the low dose group of it from P. baumii. Among them,the antitumor effect of the water extract from F. ellipsoidea(1000 mg/kg)was the best. Compared with positive and control group,the thymus index of the tested mice in the high dose group of water extract from I. hispidus showed a very significant difference(P < 0.01),but ones in the other groups had not. The water extracts from 6 “Sanghuang” fungi all can promote the expression of Bax in mouse tumor cells,and inhibit the expression of Bcl-2.

Key words: Sanghuang, Inonotus hispidus, Phellinus igniarius, Fomitiporia ellipsoidea, antitumor