CiCHI提高拟南芥总黄酮含量

doi:10.13560/j.cnki.biotech.bull.1985.2019-0669

生物技术通报 ›› 2019, Vol. 35 ›› Issue (11): 39-45.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0669

CiCHI提高拟南芥总黄酮含量

杨飞芸^{1, 2}, 白洁¹, 刘坤¹, 王瑞刚¹

1. 内蒙古农业大学生命科学学院内蒙古自治区植物逆境生理与分子生物学重点实验室,呼和浩特 010011;
2. 内蒙古农业大学食品科学与工程学院,呼和浩特 010018

收稿日期:2019-07-24 出版日期:2019-11-26 发布日期:2019-11-19
作者简介:杨飞芸,女,博士,副教授,研究方向：食品生物技术;E-mail：yangfeiyun@imau.edu.cn
基金资助:
内蒙古自治区自然科学基金项目（2017MS0354）,内蒙古自治区科技计划项目（201802083）

Increase of Flavonoids Content in Arabidopsis Heterologous Expressing CiCHI Gene

YANG Fei-yun^{1, 2}, BAI Jie¹, LIU Kun¹, WANG Rui-gang¹

1. College of Life Sciences,Inner Mongolia Agricultural University/Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology,Hohhot 010011;
2. College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018

Received:2019-07-24 Published:2019-11-26 Online:2019-11-19

摘要/Abstract

摘要： 查尔酮异构酶（CHI）是类黄酮合成途径中的第二个关键酶,对调控整个代谢途径起着重要作用,明确该基因功能,为进一步揭示中间锦鸡儿的抗逆机制提供理论依据。根据已知CHI基因的保守结构域设计简并引物,以中间锦鸡儿cDNA为模板,克隆得到CiCHI全长,并通过序列分析、系统进化分析进行确认。qRT-PCR检测分析发现,中间锦鸡儿CiCHI的表达受到紫外、NaCl、ABA等胁迫诱导。CiCHI在叶中表达量最高,茎中次之,根中最少。异源表达CiCHI使拟南芥的总黄酮含量明显增加。

关键词: 查尔酮异构酶（CHI）, 中间锦鸡儿, 总黄酮含量, 转基因拟南芥

Abstract: Chalcone isomerase（CHI）is the second key enzyme in flavonoids synthesis pathway,and plays an important role in regulating the whole metabolic pathway. Clarifying the function of CiCHI would provide the theoretical evidences for further revealing the anti-stress mechanism of Caragana intermedia. Degenerate primers were designed according to the conserved domain of known CHI genes,and the full length of CiCHI gene sequence was cloned using C. intermedia cDNA as template,then was confirmed by sequence and phylogenetic analysis. The transcript level of CiCHI was induced by ultraviolet（UV）,NaCl and abscisic acid（ABA）treatments as analyzed by quantitative real-time PCR（qRT-PCR）. The expression of CiCHI was the highest in leaves,relatively high in stems,and the lowest in roots. Heterologous expression of CiCHI significantly increased the content of total flavonoids in transgenic Arabidopsis. All these results confirmed that CiCHI was functional in transgenic Arabidopsis.

Key words: chalcone isomerase（CHI）, Caragana intermedia, Flavonoids content, transgenic Arabidopsis

杨飞芸, 白洁, 刘坤, 王瑞刚. CiCHI提高拟南芥总黄酮含量[J]. 生物技术通报, 2019, 35(11): 39-45.

YANG Fei-yun, BAI Jie, LIU Kun, WANG Rui-gang. Increase of Flavonoids Content in Arabidopsis Heterologous Expressing CiCHI Gene[J]. Biotechnology Bulletin, 2019, 35(11): 39-45.

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CiCHI提高拟南芥总黄酮含量

Increase of Flavonoids Content in Arabidopsis Heterologous Expressing CiCHI Gene

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