葡萄柚中糖基转移酶对槲皮素及其类似物催化机制探讨

doi:10.13560/j.cnki.biotech.bull.1985.2019-0057

摘要/Abstract

摘要： 研究了葡萄柚中糖基转移酶（FGT）作为催化酶,以UDP-葡萄糖、UDP-N-乙酰-D-氨基葡萄糖、UDP-甘露糖为糖基供体,槲皮素、柚皮素、柚皮苷为糖基受体,研究糖基化合成情况。通过高效液相、质谱及核磁共振氢谱对产物反应进行检测。确定了槲皮素与UDP-葡萄糖、UDP-N-乙酰-D-氨基葡萄糖可以发生糖基化反应,生成的槲皮素糖苷分子量分别为464、505。根据核磁结果鉴定,所得到的产物结构分别为槲皮素-3-O-β-D葡萄糖苷、槲皮素3-O-β-D-N-乙酰-D-氨基葡萄糖苷。以FGT酶为催化酶,对槲皮素进行了糖基化修饰。

关键词: 糖基化, 槲皮素, 槲皮素糖苷

Abstract: Using Glycosyltransferase（FGT）in grapefruit as catalytic enzyme,UDP-glucose,UDP-N-acetyl-D-glucosamine,and UDP-mannose as glycosyl donor,quercetin and naringin as glycosylation receptor,glycosylation was studied. The reaction of the product was detected by high performance liquid chromatography,mass spectrometry and nuclear magnetic resonance（NMR）hydrogen spectrum. The glycosylation reaction of quercetin with UDP-glucose and UDP-N-acetyl-D-glucosamine was confirmed. The molecular weight of quercetin glycoside was 464 and 505,respectively. According to the results of nuclear magnetic resonance,the structures of the products were quercetin-3-O-beta-D glucoside and quercetin-3-O-beta-D-N-acetyl-D-glucosamine. It was verified that FGT enzyme was used to modify quercetin glycosylation.

Key words: glycosylation, quercetin, quercetin glycoside

叶静斯, 窦文芳, 王程. 葡萄柚中糖基转移酶对槲皮素及其类似物催化机制探讨[J]. 生物技术通报, 2019, 35(7): 121-128.

YE Jing-si, DOU Wen-fang, WANG Cheng. Probe on the Catalytic Mechanism of Glycosyltransferase in Grapefruit for Quercetin and Its Analogues[J]. Biotechnology Bulletin, 2019, 35(7): 121-128.

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