解淀粉芽胞杆菌Bacillus amyloliquefaciens T-6的分离鉴定及抗病促生潜力

doi:10.13560/j.cnki.biotech.bull.1985.2020-0847

生物技术通报 ›› 2020, Vol. 36 ›› Issue (9): 202-210.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0847

• 根际微生物专题(专题主编：张瑞福研究员) • 上一篇下一篇

解淀粉芽胞杆菌Bacillus amyloliquefaciens T-6的分离鉴定及抗病促生潜力

要雅倩^1,2, 成娜娜^1,2, 李培根^1,2, 卢毅¹, 王彦刚³, 林榕姗^1,2, 周波^1,2, 王冰^1,2

1.山东农业大学,泰安 271018;
2.山东盐碱地植物-微生物联合修复工程技术研究中心,泰安 271018;
3.宁夏中青农业科技有限公司,宁夏 750000

收稿日期:2020-07-09 出版日期:2020-09-26 发布日期:2020-09-30
作者简介:要雅倩,女,硕士研究生,研究方向：资源与环境微生物;E-mail：351518530@qq.com
基金资助:
江苏泗洪县重点科技研发计划（现代农业）项目（L201804）,青岛滋百农生物技术有限公司研发项目（2018-1-1289）,山东省农业重大应用技术创新项目（SD2019ZZ009）,宁夏瓜菜产业技术协同创新中心项目（2017DC55）

Isolation and Identification of Bacillus amyloliquefaciens T-6 and Its Potential of Resisting Disease and Promoting Growth

YAO Ya-qian^1,2, CHENG Na-na^1,2, LI Pei-gen^1,2, LU Yi¹, WANG Yan-gang³, LIN Rong-shan^1,2, ZHOU Bo^1,2, WANG Bing^1,2

1. Shandong Agricultural University,Tai’an 271018;
2. Shandong Engineering Research Center of Plant-Microbe Restoration for Saline-alkali Land,Tai’an 271018;
3. Ningxia Zhongqing Agricultural Technology Co. Ltd,Yinchuan 750000

Received:2020-07-09 Published:2020-09-26 Online:2020-09-30

摘要/Abstract

摘要： 从江苏泗洪桃园发病土壤中筛选出一株对桃树根腐病有拮抗效果且抗菌范围较广泛的芽胞杆菌菌株T-6,经形态学、Biolog生理生化、16S rDNA序列与特异性PCR分析鉴定后,确定该生防菌株为解淀粉芽胞杆菌（Bacillus amyloliquefaciens）。抑菌物质酶活、促生性能测定试验表明,该菌株具备产蛋白酶、几丁质酶与溶磷、解磷和解钾的能力。盆栽试验中,T-6处理组番茄幼苗干重、根总长与叶绿素比CK组分别提高31.81%、50.79%、35.11%,防效可达54.69%。菌株T-6在生物防治桃树根腐病等土传病害方面具有较大应用潜力。

关键词: 腐皮镰刀菌, 芽胞杆菌, 桃树根腐病, 生物防治

Abstract: One Bacillus strain with antagonistic effect on peach root rot and owned a wide antibacterial range was isolated in situ from the peach orchard in Sihong,Jiangsu province. After analysis in morphology,Biolog physiology and biochemistry,16S rDNA sequence and specific PCR,the strain was identified as Bacillus amyloliquefaciens. The enzyme activity of bacteriostatic substance and growth promotion performance test revealed that the T-6 strain had the ability of producing protease,chitinase,as well as the ability of phosphorus-dissolving,phosphate-resolving and potassium-dissolving. In the pot experiment,the dry weight,total root length,and chlorophyll ratio of tomato seedlings in the T-6 treatment group increased by 31.81%,50.79%,and 35.11%,respectively,and the control effect reached 54.69%. Therefore,it is concluded that T-6 strain has great potential in biological control of soil-borne diseases such as peach root rot.

Key words: Fusarium solani, Bacillus spp., peach root rot, biological control.

要雅倩, 成娜娜, 李培根, 卢毅, 王彦刚, 林榕姗, 周波, 王冰. 解淀粉芽胞杆菌Bacillus amyloliquefaciens T-6的分离鉴定及抗病促生潜力[J]. 生物技术通报, 2020, 36(9): 202-210.

YAO Ya-qian, CHENG Na-na, LI Pei-gen, LU Yi, WANG Yan-gang, LIN Rong-shan, ZHOU Bo, WANG Bing. Isolation and Identification of Bacillus amyloliquefaciens T-6 and Its Potential of Resisting Disease and Promoting Growth[J]. Biotechnology Bulletin, 2020, 36(9): 202-210.

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解淀粉芽胞杆菌Bacillus amyloliquefaciens T-6的分离鉴定及抗病促生潜力

Isolation and Identification of Bacillus amyloliquefaciens T-6 and Its Potential of Resisting Disease and Promoting Growth

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