单细胞转录组测序技术及在哺乳动物上的应用

doi:10.13560/j.cnki.biotech.bull.1985.2022-0094

摘要/Abstract

摘要：

哺乳动物的器官是由多种细胞类型组成，它们通过细胞间的相互作用来发出信号，以维持体内平衡和确保机体发育。传统转录组测序是以大量细胞或组织为研究样本，反映的是细胞总体上转录组特征，不能分析单个细胞的基因表达情况，而单细胞RNA测序（single-cell RNA sequencing，scRNA-seq）技术的发展为揭示单个细胞转录组特征提供了有效方法。本文通过对scRNA-seq平台、scRNA-seq主要技术类型及scRNA-seq在哺乳动物上的应用展开综述。

关键词: 单细胞转录组测序, 哺乳动物, 细胞类型, 异质性

Abstract:

Mammalian organs are composed of many cell types. They send signals through cell-cell interactions to maintain homeostasis and ensure body development. Traditional transcriptome sequencing takes a large number of cells or tissues as research samples，reflecting the overall transcriptome characteristics of cells，but cannot analyze the gene expression of a single cell. The development of single cell RNA sequencing（scRNA-seq）provides an effective method for revealing the characteristics of single cell transcriptome. In this paper，the scRNA-seq platform，the main types of scRNA-seq technologies and the applications of scRNA-seq in mammals are reviewed.

Key words: single cell transcriptome sequencing, mammalian, cell types, heterogeneity

寇佳怡, 王玉玲, 曾睿琳, 兰道亮. 单细胞转录组测序技术及在哺乳动物上的应用[J]. 生物技术通报, 2022, 38(11): 41-48.

KOU Jia-yi, WANG Yu-ling, ZENG Rui-lin, LAN Dao-liang. Application of Single-cell Transcriptome Sequencing in Mammalian[J]. Biotechnology Bulletin, 2022, 38(11): 41-48.

图/表 1

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Items	10x Genomics	BD Rhapsody^TM
单细胞分离	微流控芯片，油包水	蜂巢板（scanner检测）
通量	同时测8个样本	最多可测4个样本
细胞活性	85%以上	65%以上
细胞直径	小于40 μm	小于40 μm
表面蛋白检测	可以	可以
Single Cell ATAC-SEQ	可以	不能

Items	10x Genomics	BD Rhapsody^TM
单细胞分离	微流控芯片，油包水	蜂巢板（scanner检测）
通量	同时测8个样本	最多可测4个样本
细胞活性	85%以上	65%以上
细胞直径	小于40 μm	小于40 μm
表面蛋白检测	可以	可以
Single Cell ATAC-SEQ	可以	不能