植物细胞壁结构及成像技术研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2022-1347

生物技术通报 ›› 2023, Vol. 39 ›› Issue (7): 113-122.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1347

植物细胞壁结构及成像技术研究进展

张曼¹^,²^,³^,⁴(), 张叶卓¹^,²^,³^,⁴, 何其邹洪¹^,²^,³^,⁴, 鄂一岚¹^,²^,³^,⁴, 李晔¹^,²^,³^,⁴()

1.北京林业大学生物科学与技术学院林木遗传育种全国重点实验室，北京 100083
2.北京林业大学生物科学与技术学院林木育种与生态修复国家工程研究中心，北京 100083
3.北京林业大学生物科学与技术学院林木、花卉遗传育种教育部重点实验室，北京 100083
4.北京林业大学生物科学与技术学院树木花卉育种生物工程国家林业和草原局重点实验室，北京 100083

收稿日期:2022-11-02 出版日期:2023-07-26 发布日期:2023-08-17
通讯作者: 李晔，女，副教授，研究方向：细胞壁形成机制，纳米材料作为植物基因载体；E-mail: liye0223@bjfu.edu.cn
作者简介:张曼，女，硕士研究生，研究方向：细胞壁形成机制；E-mail: ZhangMan1998@bjfu.edu.cn
基金资助:
中央高校基本科研业务费专项资金资助(2021ZY56);国家自然科学基金青年科学基金项目(31601149)

Advances in Plant Cell Wall Structure and Imaging Technology

ZHANG Man¹^,²^,³^,⁴(), ZHANG Ye-zhuo¹^,²^,³^,⁴, HE Qi-zou-hong¹^,²^,³^,⁴, E Yi-lan¹^,²^,³^,⁴, LI Ye¹^,²^,³^,⁴()

1. State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083
2. National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083
3. Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083
4. The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083

Received:2022-11-02 Published:2023-07-26 Online:2023-08-17

摘要/Abstract

摘要：

植物细胞壁作为细胞外复杂交联网络，为植物细胞生长、发育以及适应环境变化提供机械支撑，具有调节植物形态、抵抗胁迫、运输水分等功能。除此之外，植物光合作用积累的生物质大部分贮藏在细胞壁中，因此，研究细胞壁的成分和纳微结构对更好的利用植物能源具有重要意义。植物细胞壁的结构研究是当今植物界研究的前沿热点之一。随着新型成像技术的发展，近年来关于细胞壁成分和纳微结构的研究取得了阶段性的进展。本文就植物细胞壁的成分、结构、成像技术和力学性质进行了总结与展望，以期为植物细胞壁的相关研究提供新思路。

关键词: 植物细胞壁, 成分与结构, 成像技术, 力学性质

Abstract:

Plant cell wall is a complex cross-linking network that provide mechanical support for plant cells during growth, development and adaptation to a changing environment. It has the functions of regulating plant morphology, resisting stress, transporting water, etc. In addition, most of the biomass accumulated by plant photosynthesis is stored in the cell wall. Therefore, it is of great significance to study the composition and structure of the cell wall for better utilization of plant energy. The nano-microstructure of plant cell wall is one of the frontier hotspots in plant research. With the development of new imaging technologies, a large number of studies had been conducted on the composition and structure of the cell wall. In this work, we summarized the composition, structure, imaging technology and mechanical property of the plant cell wall, in order to provide new ideas for studying plant cell wall.

Key words: plant cell wall, composition and structure, imaging technology, mechanical property

张曼, 张叶卓, 何其邹洪, 鄂一岚, 李晔. 植物细胞壁结构及成像技术研究进展[J]. 生物技术通报, 2023, 39(7): 113-122.

ZHANG Man, ZHANG Ye-zhuo, HE Qi-zou-hong, E Yi-lan, LI Ye. Advances in Plant Cell Wall Structure and Imaging Technology[J]. Biotechnology Bulletin, 2023, 39(7): 113-122.

图/表 3

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技术 Technology	原理 Principle	优势 Advantages	不足 Disadvantages	参考文献 Reference
原子力显微镜 Atomic force microscope	探针与样品表面相互作用力变化来获取信息	具有原子级分辨率，样品不需要特殊处理，可在生理状态下观察	检测效率低，存在图像假象	[28⇓⇓⇓⇓⇓-34]
拉曼显微术 Raman microscopy	基于光与物质作用后发生的拉曼散射，能够获得样品分子和化学水平的结构信息	CRM非侵入性原位成像 CARS信号灵敏度增加 SRS成像快，信噪比高	CRM有荧光干扰，成像时间长 CARS具有非共振背景，信噪比低 SRS需要化学成分达到一定丰度	[35⇓⇓⇓⇓⇓⇓⇓-43]
点击化学 Click chemistry	通过简单的化学合成利用带有荧光染料的基团对多糖类似物进行标记	反应条件简单且反应迅速，可在活体状态下追踪多糖、脂质的行踪	反应过程需要铜催化，会对植物体具有毒害作用	[44-45]
傅里叶变换红外光谱技术 Fourier transform infrared spectroscopy	通过检测化合物的红外分子吸收光谱，从而对样品中的不同化合物进行鉴定	快速、无损、需样品量少	背景复杂，空间分辨率有限	[46⇓⇓⇓⇓⇓⇓⇓-54]

技术 Technology	原理 Principle	优势 Advantages	不足 Disadvantages	参考文献 Reference
原子力显微镜 Atomic force microscope	探针与样品表面相互作用力变化来获取信息	具有原子级分辨率，样品不需要特殊处理，可在生理状态下观察	检测效率低，存在图像假象	[28⇓⇓⇓⇓⇓-34]
拉曼显微术 Raman microscopy	基于光与物质作用后发生的拉曼散射，能够获得样品分子和化学水平的结构信息	CRM非侵入性原位成像 CARS信号灵敏度增加 SRS成像快，信噪比高	CRM有荧光干扰，成像时间长 CARS具有非共振背景，信噪比低 SRS需要化学成分达到一定丰度	[35⇓⇓⇓⇓⇓⇓⇓-43]
点击化学 Click chemistry	通过简单的化学合成利用带有荧光染料的基团对多糖类似物进行标记	反应条件简单且反应迅速，可在活体状态下追踪多糖、脂质的行踪	反应过程需要铜催化，会对植物体具有毒害作用	[44-45]
傅里叶变换红外光谱技术 Fourier transform infrared spectroscopy	通过检测化合物的红外分子吸收光谱，从而对样品中的不同化合物进行鉴定	快速、无损、需样品量少	背景复杂，空间分辨率有限	[46⇓⇓⇓⇓⇓⇓⇓-54]

植物细胞壁结构及成像技术研究进展

Advances in Plant Cell Wall Structure and Imaging Technology

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