作物抗ACCase抑制剂类除草剂研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2024-0251

摘要/Abstract

摘要：

杂草是影响作物产量与品质的重要不利因素之一，防除杂草是农作物田间管理必不可少的环节。乙酰辅酶A羧化酶（acetyl-coA carboxylase, ACCase）是乙酰辅酶A合成脂肪酸的限速酶，ACCase抑制剂类除草剂抑制ACCase活性，造成脂肪酸合成受阻，从而灭杀杂草。随着除草剂在农业生产上的广泛应用，抗性杂草的问题日益严重，对农作物产量和品质的影响尤为明显，创制和选育抗除草剂的优异新种质及新品种是预防田间杂草的有效策略，并对单双子叶作物复合种植具有重要意义。通过自然突变、化学诱变、转基因和基因编辑技术已在多种作物和杂草中发掘了有效变异位点，并进行了育种应用。本文针对抗ACCase抑制剂类作物种质的开发和利用，详述了ACCase的性质和作用机制、ACCase抑制剂类除草剂的分类和抗性分子机制，以及目前已发现的有效变异位点等，进而提出创制高抗ACCase抑制剂类除草剂优异新种质的高效育种策略。

关键词: 乙酰辅酶A羧化酶, ACCase抑制剂类除草剂, 复合种植, 种质创制

Abstract:

Weeds are one of the important adverse factors that seriously affect the yield and quality of crops, and weed control is an important aspect of field management. The ACCase is rate-limiting enzyme for fatty acids synthesis from acetyl-CoA, and ACCase-inhibitor herbicides inhibit ACCase activity, resulting in the obstruction of fatty acid synthesis and killing weeds. With the extensive application of herbicides in agricultural production, the issue of herbicide-resistant weeds has become increasingly serious, and the impact on crop yield and quality is particularly obvious. The development of elite herbicide-resistant germplasms and breeding new varieties with resistance to herbicide are effective strategies to control weeds in the field, especially for compound planting of mono-/dicotyledonous crops. The effective mutation sites were discovered in many crops and weeds through natural mutation, chemical mutagenesis, transgenic and gene editing techniques, and used in breeding applications. Here, we reviewed the properties and action mechanisms of ACCase in plants, the classification of ACCase-inhibitor herbicides, the resistant mechanism of ACCase, and their effective variations in crops. Finally, we proposed novel highly-effective breeding programs and strategies to improve high resistance to ACCase-inhibitor herbicides in crops.

Key words: ACCase, ACCase-inhibitor-like herbicide, compound planting, germplasm breeding

王瑶, 王荣焕, 冯铃洋, 张璐, 赵琦, 王家乐, 赵久然. 作物抗ACCase抑制剂类除草剂研究进展[J]. 生物技术通报, 2024, 40(8): 13-23.

WANG Yao, WANG Rong-huan, FENG Ling-yang, ZHANG Lu, ZHAO Qi, WANG Jia-le, ZHAO Jiu-ran. Research Progress of Crop Resistance to ACCase-inhibitor-like Herbicides[J]. Biotechnology Bulletin, 2024, 40(8): 13-23.

图/表 10

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类型 Type	主要成分 Main components
芳氧苯氧丙酸酯类 Aryloxyphenoxypropionates（FOPs）	禾草灵（diclofop-methyl），精喹禾灵（quizalofop-P-ethyl），吡氟禾草灵（fluazifop-butyl），精噁唑禾草灵（fenoxaprop-P-ethyl），喹禾灵（quizalofop-ethyl），氟吡甲禾灵（haloxyfop-P-methyl）等
环己二酮类Cyclohexanediones（DIMs）	烯草酮（clethodim），烯禾啶（sethoxydim），吡喃草酮（tepraloxydim），噻草酮（cycloxdim）等
苯基吡唑啉类Phenylpyrazoline（DEN）	唑啉草酯（pinoxaden）

类型 Type	主要成分 Main components
芳氧苯氧丙酸酯类 Aryloxyphenoxypropionates（FOPs）	禾草灵（diclofop-methyl），精喹禾灵（quizalofop-P-ethyl），吡氟禾草灵（fluazifop-butyl），精噁唑禾草灵（fenoxaprop-P-ethyl），喹禾灵（quizalofop-ethyl），氟吡甲禾灵（haloxyfop-P-methyl）等
环己二酮类Cyclohexanediones（DIMs）	烯草酮（clethodim），烯禾啶（sethoxydim），吡喃草酮（tepraloxydim），噻草酮（cycloxdim）等
苯基吡唑啉类Phenylpyrazoline（DEN）	唑啉草酯（pinoxaden）

植物种类 Plant species	变异位点 Mutant sites	FOPs类 FOPs types	DIMs类 DIMs types
看麦娘A. aequalis^[22]	I1781L	精噁唑禾草灵、炔草酯	N
日本看麦娘A. japonicus	W2027C^[23]	恶唑禾草灵、炔草酯、吡氟禾草灵、高效氟吡甲禾灵、氰氟草酯、噁唑酰草胺	N
大穗看麦娘A. myosuroides	I1781L/V^[23]	精噁唑禾草灵、炔草酯、氟吡甲禾灵	烯草酮、噻草酮
	I1781T^[24]	炔草酯	噻草酮
	D2078G^[23]	精噁唑禾草灵、炔草酯、氟吡甲禾灵	烯草酮、噻草酮
	C2088R^[25]	炔草酯、禾草灵、吡氟禾草灵、氟吡甲禾灵、喹禾灵	烯草酮、噻草酮、稀禾定、吡喃草酮
	W1999C/S^[26]	精噁唑禾草灵、炔草酯、氟吡甲禾灵	N
	W2027C^[27]	精噁唑禾草灵、炔草酯、氟吡甲禾灵	N
	I2041N/V^[28]	精噁唑禾草灵、炔草酯、氟吡甲禾灵	N
	G2096A^[29]	精噁唑禾草灵、炔草酯、氟吡甲禾灵	N
野燕麦A. fatua^[30]	I1781L	精噁唑禾草灵、炔草酯	烯草酮、噻草酮
狗尾草S. viridis^[31]	I1781L	禾草灵、氟吡甲禾灵	烯草酮、噻草酮
硬直黑麦草L. rigidum^[32]	I1781L	禾草灵、氟吡甲禾灵	烯草酮、噻草酮
	D2078G	禾草灵、氟吡甲禾灵	烯草酮、噻草酮
	C2088R	禾草灵、氟吡甲禾灵	烯草酮、噻草酮
不实野燕麦A. sterilis^[33]	I1781L	炔草酯	肟草酮
	W2027C	精噁唑禾草灵、炔草酯	N
	I2041N	精噁唑禾草灵	N
	D2078G	精噁唑禾草灵、氟吡甲禾灵	稀禾定、肟草酮
	W1999C	精噁唑禾草灵	N
棒头草P. fugax^[34]	W1999S	精噁唑禾草灵、炔草酯	稀禾定、烯草酮
细虉草P. minor^[35]	W2027C	禾草灵、精噁唑禾草灵、炔草酯	N
细虉草P. minor^[35]	D2078G	禾草灵、精噁唑禾草灵、炔草酯	N
奇虉草P. paradoxa^[36]	I1781L	炔草酯	肟草酮
奇虉草P. paradoxa^[36]	D2078G	精噁唑禾草灵、氟吡甲禾灵	稀禾定、肟草酮

植物种类 Plant species	变异位点 Mutant sites	FOPs类 FOPs types	DIMs类 DIMs types
看麦娘A. aequalis^[22]	I1781L	精噁唑禾草灵、炔草酯	N
日本看麦娘A. japonicus	W2027C^[23]	恶唑禾草灵、炔草酯、吡氟禾草灵、高效氟吡甲禾灵、氰氟草酯、噁唑酰草胺	N
大穗看麦娘A. myosuroides	I1781L/V^[23]	精噁唑禾草灵、炔草酯、氟吡甲禾灵	烯草酮、噻草酮
	I1781T^[24]	炔草酯	噻草酮
	D2078G^[23]	精噁唑禾草灵、炔草酯、氟吡甲禾灵	烯草酮、噻草酮
	C2088R^[25]	炔草酯、禾草灵、吡氟禾草灵、氟吡甲禾灵、喹禾灵	烯草酮、噻草酮、稀禾定、吡喃草酮
	W1999C/S^[26]	精噁唑禾草灵、炔草酯、氟吡甲禾灵	N
	W2027C^[27]	精噁唑禾草灵、炔草酯、氟吡甲禾灵	N
	I2041N/V^[28]	精噁唑禾草灵、炔草酯、氟吡甲禾灵	N
	G2096A^[29]	精噁唑禾草灵、炔草酯、氟吡甲禾灵	N
野燕麦A. fatua^[30]	I1781L	精噁唑禾草灵、炔草酯	烯草酮、噻草酮
狗尾草S. viridis^[31]	I1781L	禾草灵、氟吡甲禾灵	烯草酮、噻草酮
硬直黑麦草L. rigidum^[32]	I1781L	禾草灵、氟吡甲禾灵	烯草酮、噻草酮
	D2078G	禾草灵、氟吡甲禾灵	烯草酮、噻草酮
	C2088R	禾草灵、氟吡甲禾灵	烯草酮、噻草酮
不实野燕麦A. sterilis^[33]	I1781L	炔草酯	肟草酮
	W2027C	精噁唑禾草灵、炔草酯	N
	I2041N	精噁唑禾草灵	N
	D2078G	精噁唑禾草灵、氟吡甲禾灵	稀禾定、肟草酮
	W1999C	精噁唑禾草灵	N
棒头草P. fugax^[34]	W1999S	精噁唑禾草灵、炔草酯	稀禾定、烯草酮
细虉草P. minor^[35]	W2027C	禾草灵、精噁唑禾草灵、炔草酯	N
细虉草P. minor^[35]	D2078G	禾草灵、精噁唑禾草灵、炔草酯	N
奇虉草P. paradoxa^[36]	I1781L	炔草酯	肟草酮
奇虉草P. paradoxa^[36]	D2078G	精噁唑禾草灵、氟吡甲禾灵	稀禾定、肟草酮

植物种类Plant species	专利授权或公开号Patent license or publicationed code	变异位点Mutation site	FOPs类 FOPs type	DIMs类 DIMs type
小麦T. aestivum	WO2012106321A1	A2004V	R	N
水稻O. sativa	CN112410308A	W2010L+ C2099R	R	N
水稻O. sativa	CN108359646A	I1792L	R	N
		A2015V	R	N
		W2038C	R	N
		I2052N	R	N
		D2089G	R	N
		I1792L+A2015V	R	N
		A2015V+W2038C	R	N
		I1792L+W2038C	R	N
水稻O. sativa	CN201810138888.3	N1791S	R	R
		N1791S+I1792L	R	R
		N1791S+G2107S	R	R