红球菌Rhodococcus. sp G14生物转化合成2-苯 基丙酸

生物技术通报 ›› 2013, Vol. 0 ›› Issue (12): 173-177.

红球菌Rhodococcus. sp G14生物转化合成2-苯基丙酸

左可, 李志敏, 叶勤

华东理工大学生物反应器工程国家重点实验室，上海 200237

收稿日期:2013-06-17 出版日期:2013-12-20 发布日期:2013-12-20
作者简介:左可，男，硕士研究生，研究方向：生物催化；E-mail：zuoke2006@126.com

Production of 2-phenylpropionic Acid by Rhodococcus sp. G14

Zuo Ke, Li Zhimin, Ye Qin

State Key Laboratory of Bioreactor Engineering，East China University of Science and Technology，Shanghai 200237

Received:2013-06-17 Published:2013-12-20 Online:2013-12-20

摘要/Abstract

摘要： 2-苯基丙酸作为布洛芬合成过程中的一种重要的医药中间体，目前对其的生产及应用正在逐年增加，但价格十分昂贵。对野生红球菌Rhodococcus sp. G14生物催化异丙苯合成2-苯基丙酸的条件进行探索。利用响应面优化方法，得到菌体生长最适培养基，分批培养OD₆₀₀ 可达到19.82。单因素试验优化菌体反应时间、反应温度、反应菌浓、底物浓度、渗透处理，获得最佳催化条件下2-苯基丙酸产量为14.30 mg/L，为后续优化提高打下了基础。

关键词: 生物催化, 异丙苯, 2-苯, 基丙酸, 优化

Abstract: As a kind of important medicine intermediate in ibuprofen synthesis, the demand and application of 2-phenylpropionic acid(2-PPA)is increased year by year. In this study, the reaction conditions for the biotransformation of cumune to 2-PPA by wild type Rhodococcus sp. G14 was investigated. Using response surface optimization, the best medium condition for growth which could result 19.82 of OD600 in batch cultivation was obtained. After single factor experiment on reaction time, reaction temperature, OD₆₀₀, concentration of substrate, osmotic treatment, 14.30 mg/L of 2-PPA was obtained, which would benefit the subsequent optimization.

Key words: Biotransformation, Cumene 2-phenylpropionic acid, Optimization

左可, 李志敏, 叶勤. 红球菌Rhodococcus. sp G14生物转化合成2-苯基丙酸[J]. 生物技术通报, 2013, 0(12): 173-177.

Zuo Ke, Li Zhimin, Ye Qin. Production of 2-phenylpropionic Acid by Rhodococcus sp. G14[J]. Biotechnology Bulletin, 2013, 0(12): 173-177.

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