MicroRNA-21通过正调控ERK1/2通路促进食管鳞状 细胞癌的增殖和迁移

生物技术通报 ›› 2013, Vol. 0 ›› Issue (12): 189-193.

MicroRNA-21通过正调控ERK1/2通路促进食管鳞状细胞癌的增殖和迁移

刘凤霞¹ 张盼盼¹ 李建勇¹ 郑树涛² 卢晓梅² 刘文亚³

1.新疆医科大学人体解剖学教研室，乌鲁木齐 830011
2.新疆医科大学第一附属医院医学研究中心，乌鲁木齐 830011
3.新疆医科大学第一附属医院影响中心，乌鲁木齐 830011

收稿日期:2013-06-15 出版日期:2013-12-20 发布日期:2013-12-20
作者简介:刘凤霞，女，博士研究生，副教授，研究方向：解剖教学和分子病理学；E-mail：liufengxia555@126.com
基金资助:
新疆自治区科技厅面上项目（2012211A080）

MicroRNA-21 Promotes the Proliferation and Migration of Esophageal Squamous Carcinoma Cell via Activating ERK1/2/MAPK Pathway

Liu Fengxia¹, Zhang Panpan¹, Li Jianyong¹, Zheng Shutao², Lu Xiaomei², Liu Wenya³

1. Department of Anatomy，College of Basic Medicine，Xinjiang University of Medical Science，Urumqi 830011
2. Medical Research Center，the First Affiliated Hospital of Xinjiang Medical University，Urumqi 830011
3. Department of Radiology，the First Affiliated Hospital of Xinjiang Medical University ，Urumqi 830011

Received:2013-06-15 Published:2013-12-20 Online:2013-12-20

摘要/Abstract

摘要： 旨在对食管鳞癌（ESCC）中miR-21调控细胞增殖和侵袭的机制进行探讨。平板克隆试验和伤口愈合体外试验发现，miR-21过表达可促进细胞增殖和迁移，相反，沉默miR-21可抑制细胞增殖和迁移。同时，Western blotting试验发现，miR-21过表达的Eca109细胞中p-ERK1/2表达上调，而在miR-21抑制的Eca109细胞中表达下调。试验结果提示miR-21可能通过激活ERK1/2/MAPK信号通路促进ESCC细胞增殖和侵袭，而且miR-21是在转录后水平正调控ERK1/2/MAPK信号通路。

关键词: microRNA-21, ERK1/2, 增殖, 迁移, 食管鳞状细胞癌

Abstract: It was to analyse the underlying mechanism of how miR-21 promotes proliferation and invasion in ESCC. Both plate colony formation and wound-healing assays showed that proliferation and migration of ESCC cell line Eca109 were increased in miR-21 mimics group and decreased in miR-21 inhibitor group after transfection into Eca109 cells with miR-21 mimics, miR-21 inhibitor and scramble sequence, respectively. Meanwhile, Western blotting results showed p-ERK1/2 expression was elevated in miR-21 mimics group, whereas decreased in miR-21 inhibitor group. The findings demonstrated that miR-21 promotes the proliferation and migration of Eca109 cell through activating ERK1/2/MAPK pathway；miR-21 can positively regulate and control the ERK1/2/MAPK signaling pathway at post-transcriptional level in Eca109 cells.

Key words: MicroRNA-21, ERK1/2, Proliferation, Migration, Esophageal squamous cell carcinoma

刘凤霞, 张盼盼, 李建勇, 郑树涛, 卢晓梅, 刘文亚. MicroRNA-21通过正调控ERK1/2通路促进食管鳞状细胞癌的增殖和迁移[J]. 生物技术通报, 2013, 0(12): 189-193.

Liu Fengxia, Zhang Panpan, Li Jianyong, Zheng Shutao, Lu Xiaomei, Liu Wenya. MicroRNA-21 Promotes the Proliferation and Migration of Esophageal Squamous Carcinoma Cell via Activating ERK1/2/MAPK Pathway[J]. Biotechnology Bulletin, 2013, 0(12): 189-193.

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