河西走廊盐碱土壤中抗立枯丝核菌的放线菌筛选

生物技术通报 ›› 2014, Vol. 0 ›› Issue (1): 156-160.

河西走廊盐碱土壤中抗立枯丝核菌的放线菌筛选

王蓓,牛世全,达文燕,李海云,胡娇龙,赵国杰

西北师范大学生命科学学院旱区微生物资源与生态研究所，兰州 730070

收稿日期:2013-09-16 出版日期:2014-01-23 发布日期:2014-01-23
作者简介:王蓓，女，硕士研究生，研究方向: 微生物拮抗性；E-mail: wangbei0523@126.com
基金资助:
国家自然科学基金资助项目(31260134，30960078)

Screening of Actinomyces on Antagonism to Rhizoctonia solani Isolated from Saline-alkali Soils in Hexi Corridor

Wang Bei Niu Shiquan Da Wenyan Li Haiyun Hu Jiaolong Zhao Guojie

Institude of Arid Microbial Resources and Ecology， College of Life Science，Northwest Normal University，Lanzhou 730070

Received:2013-09-16 Published:2014-01-23 Online:2014-01-23

摘要/Abstract

摘要： 从甘肃省河西走廊盐碱土中分离所得50 株放线菌中，筛选出对立枯丝核菌有生防潜能的放线菌，旨在为生物防治#br#作物病害提供新的菌种资源。通过平板对峙法、生长速率法及活体组织法筛选拮抗菌，并对其进行形态及分子鉴定。经过平板初筛，#br#有4 株菌株对立枯丝核菌有拮抗作用，编号Ⅰ 18-5-2、Ⅲ 22-3-12、Ⅳ 16-3-2、DC009-1-23，其中Ⅳ 16-3-2 和DC009-1-23 有较高#br#的#br#拮抗性，抑菌圈直径分别为20.5 mm 和15.5 mm ；进而对这2 菌株进行发酵液复筛，菌丝的生长抑制率分别为89.02% 和80.49% ；#br#活体组织试验表明，Ⅳ 16-3-2 和DC009-1-23 对立枯丝核菌的防治效果分别达54.29% 和45.71% ；通过形态培养特征和16S rDNA#br#序列鉴定，将菌株Ⅳ 16-3-2 初步鉴定为变异链霉菌，DC009-1-23 为丁香链霉菌。

关键词: 立枯丝核菌, 放线菌, 盐碱地, 生物防治

Abstract: This study selected several actinomycetes from 50 strains of the saline-alkali soils of Hexi Corridor in Gansu Province which have been proved to be resistant to Rhizoctonia solani by antagonistic test, which would provide new strains resources in crop disease control. Pair culture method, growth rate method and tissue culture method were used to select antagonistic bacteria. The results of antagonistic test showed that there were 4 strains with antagonism to Rhizoctonia solani, which were named Ⅰ 18-5-2, Ⅲ 22-3-12, Ⅳ 16 -3-2 and DC009-1-23. From above four strains, Ⅳ 16-3-2 and DC009-1-23 had a higher antagonism and the diameters of inhibiting bacteria circle were 20.5 mm and 15.5 mm. By rescreening these two strains with higher antagonism from fermentation broth, the result showed that their mycelial growth inhibition rates were 89.02% and 80.49%. Biopsy tests showed that the control efficiency of IV 16-3-2 and DC009-1-23 to Rhizoctonia solani reached 54.29% and 45.71%, respectively. According to morphological characteristic and phylogenetic analysis based on 16S rRNA gene sequence, the strain Ⅳ 16-3-2 was preliminarily identified to be Streptomyces variabilis while the strain DC009-1-23 was identified to be Streptomyces lilaceus.

Key words: Rhizoctonia solani, Actinomycetes, Saline-alkali soils, Biological control

王蓓,牛世全,达文燕,李海云,胡娇龙,赵国杰. 河西走廊盐碱土壤中抗立枯丝核菌的放线菌筛选[J]. 生物技术通报, 2014, 0(1): 156-160.

Wang Bei Niu Shiquan Da Wenyan Li Haiyun Hu Jiaolong Zhao Guojie. Screening of Actinomyces on Antagonism to Rhizoctonia solani Isolated from Saline-alkali Soils in Hexi Corridor[J]. Biotechnology Bulletin, 2014, 0(1): 156-160.

参考文献

[1] 何迎春, 高必达. 立枯丝核菌的生物防治[J]. 中国生物防治,2000, 16(1): 31-34.
[2] 张楷正. 立枯丝核菌拮抗菌的分离鉴定及其生理特性研究[J].北方园艺, 2010(21): 39-41.
[3] 田连生, 张根伟, 黄亚丽. 木霉对蔬菜立枯丝核菌病害的生防效果研究[J]. 河北省科学院学报, 2002, 19(4): 254-256.
[4] 方羽生, 杨卫华, 张洪玲, 等. 放线菌对4 种病原真菌的拮抗作用初探[J]. 广东农业科学, 2001(5): 6-8.
[5] 韩立荣, 赵利刚, 顾彪, 等. 5 株放线菌对9 种靶标病原真菌的持续抑菌作用[J]. 西北农林科技大学学报, 2006, 34(2): 53-60.
[6] Li D, Alexander M. Faciors affecting co-inoculation with abtibiotic producing bacteria to enhance rhizobial colonization and nodulation[J]. Plant and Soil, 1991, 131: 195-201.
[7] Hu X, Roberts DP, Maul JE, et al. Formalations of endophytic bacterium Bacillus subtilis Tu-100 suppress Sclerotinia sclerotiruni on oilseed rape and improve plant vigor in field trials conducted at separate locations[J]. Can J Microbiol, 2011, 57: 539-546.
[8] Schroth MN, Hancock JG. Disease-suppressive soil and rootcolonizating bacteria[J]. Sciences, 1982, 216: 1370-1371.
[9] Kloepper JW. Host specificity in microbe-microbe interactions[J].BioScience, 1996, 46: 406-409.
[10] 贾雨, 贾丽苑, 黄建新. 放线菌对植物病害的防治作用及应用[J]. 西安文理学院学报, 2012, 15(3): 6-10.
[11] 欧雄常, 柳凤, 詹儒林. 拮抗辣椒疫病菌的红树内生细菌筛选及RS261 菌株鉴定[J]. 微生物学通报, 2009, 36(2): 175- 180.
[12] 申端玉, 魏少鹏, 姬志勤, 等. 放线菌A19 菌株次生代谢产物的初步研究[J]. 西北农林科技大学学报: 自然科学版,2008, 36(1): 173-178.
[13] 张玲玲, 董美玉, 许凤春. 放线菌C3-11 的抗菌活性筛选及发酵液稳定性研究[J]. 现代农业科技, 2009, 3: 109-113.
[14] 许凤春, 董美玉, 张玲玲, 等. 放线菌Gl-8 菌株发酵液抗菌谱及稳定性的研究[J]. 生物技术, 2008, 18(4): 81-83.
[15] 马海丹, 周文明, 吴绍东. 拮抗放线菌M18 的分离鉴定及杀菌活性研究[J]. 西北农业学报, 2007, 16(5): 291-294.
[16] 王群利, 师宝君, 卞小莹, 等. 土壤拮抗放线菌的分离及其抗菌活性筛选[J]. 植物保护学报, 2008, 34(3): 41-46.
[17] 李东平, 龙建友, 胡兆农, 等. 放线菌LDP-18 发酵液杀菌活性研究[J]. 西北农业学报, 2005, 14(1): 102-105.
[18] 李璐宁, 张薇, 赵永强, 等. 放线菌Y23 菌株发酵液抗菌活性及稳定性测定[J]. 山东农业科学, 2009, 1: 71-74.
[19] 慕立义. 植物化学保护研究方法[M]. 北京: 中国农业出版社,1991: 79-81.
[20] 刘奎, 姚刚, 符悦冠. 新入侵害虫螺旋粉虱的田间药效试验及防效[J]. 热带作物学, 2008, 29(2): 220-224.
[21] 黄大林, 袁桂峰, 徐雅娟, 等. 广西北海红树林土壤放线菌 BH0954 的分离和鉴定[J]. 中国农学通报, 2010(18): 2-6.
[22] 中国科学院微生物研究所放线菌分类组. 链霉菌鉴定手册[M]. 北京: 科学出版社, 1975.
[23] 姜云, 黄丽丽, 陈长卿, 等. 一株拮抗番茄叶霉病菌的放线菌筛选、鉴定及发酵条件研究[J]. 微生物学报, 2007, 47(4): 622-627.
[24] 王燕, 宗兆锋, 程联社. 放线菌在植物病害生物防治中的应用[J]. 2005, 4(3): 21-23.