罗氏沼虾中副溶血弧菌群体感应的检测

生物技术通报 ›› 2014, Vol. 0 ›› Issue (3): 146-150.

罗氏沼虾中副溶血弧菌群体感应的检测

汪映, 朱素芹, 张彩丽, 曾名湧

(中国海洋大学食品科学与工程学院，青岛 266003)

收稿日期:2013-10-14 出版日期:2014-03-29 发布日期:2014-03-31
作者简介:汪映，男，硕士研究生，研究方向:副溶血弧菌群体感应；E-mail:austinwang1020@foxmail.com
基金资助:
国家自然科学基金项目(31071613)，十二五国家科技支撑计划(2012BAD28B05)

Detection of Quorum Sensing in Vibrio parahaemolyticus Isolated from Macrobrachium rosenbergii

Wang Ying, Zhu Suqin, Zhang Caili, Zeng Mingyong

(College of Food Science and Engineering, Ocean University of China，Qingdao 266003)

Received:2013-10-14 Published:2014-03-29 Online:2014-03-31

摘要/Abstract

摘要： 利用3种不同的哈维氏弧菌报告菌株 V. harveyi JMH612、 V.harveyi JMH597和 V.harveyi JAF375检测了罗氏沼虾体内分离的副溶血弧菌VIB461和VIB800的群体感应信号分子，同时用PCR扩增了两株菌调控AI-2型信号分子分泌的 LuxS基因。结果表明，两株菌均能产生3种类型的信号分子:HAI-1、AI-2和CAI-1。3种信号分子的活性均具有生长阶段依赖性，在对数生长初期出现并在对数生长后期或稳定期前期达到最高水平，然后随着培养时间的延长呈现一定的下降。两株副溶血弧菌的PCR扩增片段的测序结果与已上传的副溶血弧菌的 LuxS基因序列的相似度均在95%以上，说明两株副溶血弧菌都含有 LuxS基因。

关键词: 副溶血弧菌, 群体感应, HAI-1类信号分子, AI-2类信号分子, LuxS基因, CAI-1类信号分子

Abstract: Vibrio parahaemolyticus VIB461 and VIB800, which were isolated from Macrobrachium rosenbergii, were found to produce three kinds of quorum sensing signals using three different quorum sensing reporter strains V.harveyi JMH612, V.harveyi JMH597 and V.harveyi JAF375, and LuxS gene of the two strains were also amplified by PCR. The results showed that both strains produced three kinds of singnal molecules:HAI-1, AI-2 and CAI-1. The level of three signal molecules all ascended with an increase in bacterial population density, reached high levels during the late exponential phase or early stationary phase, and decreased afterwards. The PCR amplification sequences both shared more than 95% similarity with the reported LuxS gene of V. Parahaemolyticus, which indicated that both strains harbored LuxS gene.

Key words: Vibrio parahaemolyticus, Quorum sensing, HAI-1, AI-2, LuxS, gene CAI-1

汪映, 朱素芹, 张彩丽, 曾名湧. 罗氏沼虾中副溶血弧菌群体感应的检测[J]. 生物技术通报, 2014, 0(3): 146-150.

Wang Ying, Zhu Suqin, Zhang Caili, Zeng Mingyong. Detection of Quorum Sensing in Vibrio parahaemolyticus Isolated from Macrobrachium rosenbergii[J]. Biotechnology Bulletin, 2014, 0(3): 146-150.

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