一株解淀粉芽孢杆菌的分离鉴定及其抑菌条件研究

doi:10.13560/j.cnki.biotech.bull.1985.2015.09.024

摘要/Abstract

摘要： 旨在从海洋环境中分离筛选抑制鲈鱼病原鳗弧菌的拮抗菌，进行菌种鉴定，并对其抑菌条件及药物敏感性进行研究。采用十字交叉划线法和平板点种法筛选鲈鱼病原鳗弧菌的拮抗菌，采用形态学、生理生化反应及16S rDNA基因序列的系统发育分析方法对分离拮抗菌进行鉴定，采用单因子变量法对拮抗菌WTD的最佳抑菌条件进行研究，采用平板药敏纸片法对其药物敏感性进行研究。结果显示，从海洋环境样品中分离出126株细菌，筛选出19株拮抗菌。其中菌株WTD的拮抗效果最强，细菌鉴定结果表明，该菌为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。最佳抑菌条件研究表明，该菌在温度35℃、pH7、盐度为1% NaCl的时候，拮抗效果最好。药敏实验结果表明，该菌对麦迪霉素等29种抗生素敏感，而对多粘菌素具有抗性。拮抗菌解淀粉芽孢杆菌WTD对鲈鱼病原菌鳗弧菌有较强的抑制作用，具有潜在的应用价值。

关键词: 解淀粉芽孢杆菌, 鳗弧菌, 抑菌作用, 拮抗菌

Abstract: This work is to isolate and screen the antagonistic bacteria from sea environment which can inhibit the growth of the seaperch’s pathogenic bacterium Vibrio anguillarum W-1, and investigate the optimal conditions of its antimicrobial activity as well as the drug sensitivity. The methods of plate crossing streaking and point-inoculating were used to screen the antagonistic bacteria. The morphology, physiological and biochemical reactions and 16S rDNA phylogenetic analysis were applied for the identification of antagonistic bacteria. The one-variable method was utilized to optimize the antimicrobial conditions of the antagonistic bacteria. Total 126 strains of bacteria were isolated from the coast of Qingdao, Xiamen and Shidao, and 19 antagonistic bacteria were screened. The bacterial strain WTD with the optimal antagonistic activity was identified as Bacillus amyloliquefaciens. Under the optimal conditions of 35℃, salinity 1% NaCl, and pH 7, the antagonistic effect was the best. The drug sensitivity test revealed that the bacterium was sensitive to 29 antibiotics such as midecamycin, but resistant to polymyxin. As a conclusion, the antagonistic strain B. amyloliquefaciens WTD can efficiently inhibit the growth of the seaperch’s pathogenic bacterium V. anguillarum, and has the potential application value in the aquaculture.

Key words: Bacillus amyloliquefaciens, Vibrio anguillarum, antimicrobial activity, antagonistic bacteria

张超, 王祥红, 徐涛, 李帅, 钱浩, 李帅, 张增虎. 一株解淀粉芽孢杆菌的分离鉴定及其抑菌条件研究[J]. 生物技术通报, 2015, 31(9): 170-176.

Zhang Chao, Wang Xianghong, Xu Tao, Li Shuai, Qian Hao, Li Shuai, Zhang Zenghu. Isolation and Identification of Bacillus amyloliquefaciens WTD and the Optimal Conditions of Its Antimicrobial Activity[J]. Biotechnology Bulletin, 2015, 31(9): 170-176.

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