一株沙雷氏菌的分离及其群体感应现象

生物技术通报 ›› 2014, Vol. 0 ›› Issue (7): 150-155.

一株沙雷氏菌的分离及其群体感应现象

张彩丽, 朱素琴, 汪映, 孙秀娇, 曾名湧

中国海洋大学食品科学与工程学院, 青岛 266003

收稿日期:2013-12-18 出版日期:2014-07-15 发布日期:2014-07-16
作者简介:张彩丽, 女, 硕士研究生, 研究方向：水产品高值化利用；E-mail：ncuskzhangcaili@163.com
基金资助:
国家自然科学基金项目（31071613）, 十二五国家科技支撑计划（2012BAD28B05）

Isolation, Identification and Quorum Sensing of a Serratia spp.

Zhang Caili, Zhu Suqin, Wang Ying, Sun Xiujiao, Zeng Mingyong

College of Food Science and Engineering, Ocean University of China, Qingdao 266003

Received:2013-12-18 Published:2014-07-15 Online:2014-07-16

摘要/Abstract

摘要： 从腐败的凡纳滨对虾中分离得到一株具有群体感应的菌株, 利用16S rRNA和生理生化试验鉴定其为黏质沙雷氏菌, 但不产灵红素, 命名为Serratia marcescens AK1。通过生物检测方法对菌株AK1进行群体感应检测, 薄层层析法鉴定该菌株的信号分子类型。结合菌株生长规律, 测定不同时间段信号分子的含量。结果显示, 菌株AK1能诱导紫色杆菌CV026产生紫色杆菌素, 诱导根癌农杆菌A136分解X-gal产生蓝色；薄层层析检测结果显示该菌能产生两种群体感应信号分子C6-HSL和3-oxo-C6-HSL, 并具有密度依赖性, 信号分子含量在生长对数后期达到最大值。

关键词: 沙雷氏菌, 鉴定, 群体感应, N-酰基高丝氨酸内酯

Abstract: One quorum sensing strain was isolated from spoilage Litopenaeus vannamei. Specie was determined by 16S rRNA gene analysis and classical tests, it can not produce red pigment on medium, named it Serratia marcescens AK1.Quorum sensing was detected by reporter strains and signal molecules types were identified by thin layer chromatography. Quantitative analysis of the signaling molecule secreted by AK1 during different growth stages. The result indicates strain AK1 can induce Chromobacterium violaceum CV026 and Agrobacterium tumefaciens A136 produce purple and blue color respectively. Thin layer chromatography showed that AK1 can produce C6-HSL and 3-oxo-C6-HSL types of signaling molecules with density-dependent. They reached the maximum in the late logarithmic phase.

Key words: Serratia marcescens, Identification, Quorum sensing, N-acyl-homoserine lactones

张彩丽, 朱素琴, 汪映, 孙秀娇, 曾名湧. 一株沙雷氏菌的分离及其群体感应现象[J]. 生物技术通报, 2014, 0(7): 150-155.

Zhang Caili, Zhu Suqin, Wang Ying, Sun Xiujiao, Zeng Mingyong. Isolation, Identification and Quorum Sensing of a Serratia spp.[J]. Biotechnology Bulletin, 2014, 0(7): 150-155.

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