OsPHGPx 基因的过量表达增强水稻抗氧化能力

摘要/Abstract

摘要： 植物在生物或非生物胁迫下会通过表达磷脂氢谷胱甘肽过氧化物酶（PHGPx）来抵御胁迫引起的氧化损伤,但是PHGPx在植物体内抗氧化途径中所扮演的生理角色目前尚不完全清楚。利用农杆菌遗传转化技术,构建了过表达OsPHGPx基因的转基因水稻,并对转基因水稻进行了PCR、实时定量PCR以及Western blot等检测分析,结果表明OsPHGPx基因已成功转入水稻并正常表达。与野生型水稻相比,这些过量表达OsPHGPx的转基因水稻抵御百草枯氧化伤害的能力提高。

关键词: OsPHGPx, ROS, 遗传转化, 转基因水稻

Abstract: Plants can express phospholipid hydroperoxide glutathione peroxidase（PHGPx）to resist oxidative stress caused by biotic or abiotic stress. But the physiological role of PHGPx in conferring stress defence is still unclear. In this study, agrobacterium genetic transformation technology was successfully used to obtain transgenic rice overexpressing OsPHGPx. PCR, Real-time PCR and Western blot analysis was carried out on the transgenic rice, and all the results showed that OsPHGPx gene was transformed into the rice successfully. In addition, compared with the non-transgenic rice, seedlings of transgenic lines demonstrated enhanced tolerance to oxidative stress caused by paraquat.

Key words: OsPHGPx , ROS , Genetic transformation , Transgenic rice

宋建辉,李甜,吴秀云,吴赴清,刘进元. OsPHGPx 基因的过量表达增强水稻抗氧化能力[J]. 生物技术通报, 2014, 0(11): 107-113.

Song Jianhui,Li Tian,Wu Xiuyun,Wu Fuqing,Liu Jinyuan. Enhanced Oxidative Stress Tolerance of Transgenic Rice Plants Overexpressing OsPHGPx Gene[J]. Biotechnology Bulletin, 2014, 0(11): 107-113.

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