重组酵母产人IFN-α2a与LL-37融合蛋白的工艺优化

生物技术通报 ›› 2014, Vol. 0 ›› Issue (11): 214-218.

重组酵母产人IFN-α2a与LL-37融合蛋白的工艺优化

张明杰

广东紫金正天药业有限公司,河源 517000

收稿日期:2014-03-18 出版日期:2014-11-07 发布日期:2014-11-07
作者简介:张明杰,教授,研究方向：微生物工程技术
基金资助:
广东省重大科技专项（2011A080502011）

The Process Optimization of Pichia pastoris GS115LI for Producing Fused Protein of LL-37 and IFN-α2a

Zhang Mingjie

Pharmaceutical Co., Ltd. Guangdong Zijing Zhengtian, Heyuan 517000

Received:2014-03-18 Published:2014-11-07 Online:2014-11-07

摘要/Abstract

摘要： 通过单因素试验探索重组酵母P. pastoris GS115LI产人LL-37基因与IFN-α2a融合蛋白的最佳条件。结果显示,重组酵母菌产蛋白酶的最佳表达条件是利用BMMY为诱导培养基,以接种量OD₆₀₀=5.5、温度26℃、pH6.0、诱导剂（甲醇）为每隔24 h添加1.0%、振荡速度为200 r/min条件下连续诱导144 h。在最佳培养条件下,发酵液中的LL-37最高抗菌活性达27.9 mm。与初始的发酵条件相比,人LL-37基因与IFN-α2a融合蛋白的产量提高了32.29%。

关键词: 干扰素α2a, LL-37, 工艺

Abstract: With experiments of single factor, the optimal process of recombinant P. pastoris GS115LI for producing fused protein of LL-37 and IFN-α2a were using BMMY as inducing medium, inoculum as OD₆₀₀=5.5, temperature as 26℃, pH6.0, inducing chemical（methanol）adding amount as 1.0% every 24 h, incubating shaking speed as 200 r/min, and inducing time as 144 h. Under those optimal conditions, the 27.9 mm of LL-37 antimicrobial activity could be gotten. Compared with the original conditions, the productivity of fused protein of LL-37 and IFN-α2a was increased as 32.29%.

Key words: Interferon-α2a , LL-37 , Fermentation process

张明杰. 重组酵母产人IFN-α2a与LL-37融合蛋白的工艺优化[J]. 生物技术通报, 2014, 0(11): 214-218.

Zhang Mingjie. The Process Optimization of Pichia pastoris GS115LI for Producing Fused Protein of LL-37 and IFN-α2a[J]. Biotechnology Bulletin, 2014, 0(11): 214-218.

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