葡萄酒酿酒酵母果糖利用影响因素的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2014.12.005

摘要/Abstract

摘要： 葡萄酒酒精发酵后期，酿酒酵母果糖利用能力与酒精发酵中止和发酵不彻底密切相关，而且残余果糖还可能带来微生物污染和酒体失衡的危险。从酿酒酵母己糖跨膜转运、己糖胞内磷酸化及发酵条件(葡萄糖浓度、乙醇和温度)对果糖利用影响等方面的研究进展进行了综述，对筛选和构建高果糖利用优良葡萄酒酿酒酵母研究，以及生产中合理控制酒精发酵过程具有重要指导意义。

关键词: 葡萄酒, 酿酒酵母, 果糖

Abstract: Yeasts with a high fructose consumption capability are very important for winemakers to solve problems associated with sluggish or stuck fermentations. Residual fructose at end of fermentation also can cause undesirable sweetness and risk of microbiological spoilage in wines. The fructose consumption capability is dependent on the yeast’s genetic background and on external conditions. It was reviewed on the level of the transporters cross the cellular membrane, hexose phosphorylation, and the effects of external factors(glucose concentration, ethanol stress, temperature and nitrogen resources availability). It is of significant importance in winemaking to find out and control the key factors that can have an effect on fructose utilization at end of fermentation.

Key words: Wine, Saccharomyces cerevisiae, Fructose

赵文英 ,贾万利. 葡萄酒酿酒酵母果糖利用影响因素的研究进展[J]. 生物技术通报, 2014, 0(12): 29-32.

Zhao Wenying, Jia Wanli. Advance Research on Factors of Fructose Utilization in the Wine Yeast Saccharomyces cerevisiae[J]. Biotechnology Bulletin, 2014, 0(12): 29-32.

参考文献

[1] Dumont A, Raynal C, Raginel F, et al. The ability of wine yeast to consume fructose[J]. Australian and New Zealand Grapegrower and Winemaker, 2009, 543: 52-57.
[2] Gafner J, Schütz M. Impact of glucose-fructose-ratio on stuck ferme-ntations: practical experiences to restart stuck fermentations[J]. Viticulture and Enology Science, 1996, 51: 214-218.
[3] 陈莹, 屈慧鸽, Gafber J. 葡萄糖-果糖比对发酵停滞的影响及其预防、重启策略[J]. 中外葡萄与葡萄酒, 2010, 5: 69-71.
[4] Arroyo-Lopez N, Querol A, Barrio E. Application of a substrate inhibition model to estimate the effect of fructose concentration on the growth of diverse Saccharomyces cerevisiae strains[J]. Journal of Industrial Microbiology & Biotechnology, 2009, 36: 663-669.
[5] Berthels NJ, Cordero Otero RR, Buer FF, et al. Discrepancy in glucose and fructose utilisation during fermentation by Saccharomyces cerevisiae wine yeast strains[J]. FEMS Yeast Research, 2004, 4: 683-689.
[6] Liccioli T, Chambers P, Jiranek V, et al. A novel methodology independent of fermentation rate for assessment of the fructophilic Character of wine yeast strains[J]. Journal of Industrial Microbiology & Biotechnology, 2011, 38(7)：833-843.
[7] 赵文英, 高莉, 贾万利. 葡萄酒酿酒酵母嗜果糖特性的评价方法[J]. 微生物学通报, 2013, 06: 1027-1032.
[8] Luyten K, Riou C, Blondin B. The hexose transporters of Saccharomyces cerevisiae play di erent roles during enological fermentation[J]. Yeast, 2002, 19: 1-15.
[9] Lin Z, Li WH. Expansion of hexose transporter genes was associated with the evolution of aerobic fermentation in yeasts[J]. Molecular Biology and Evolution, 2011, 28: 131-142.
[10] Perez M, Luyten K. Analysis of Saccharomyces cerevisiae hexose carrier expression during wine fermentation: Both low-and high-affinity Hxt transporters are expressed[J]. FEMS Yeast Research, 2005, 5: 351-361.
[11] Karpel JE, Place WR, Bisson LF. Analysis of the major hexose transporter genes in wine strains of Saccharomyces cerevisiae[J]. American Journal of Enology and Viticulture, 2008, 59: 265-275.
[12] Guillaume C, Delobel P, Sablayrolles JM, et al. Molecular basis of fructose utilization by the wine yeast Saccharomyces cerevisiae: a mutated HXT3 allele enhances fructose fermentation[J]. Appl Environ Microbiol, 2007, 73: 2432-2439.
[13] Galeote V, Novo M, Salema-Oom, et al. FSY1, a horizontally transferred gene in the Saccharomyces cerevisiae EC1118 wine yeast strain, encodes a high-affinity fructose/H+ symporter[J]. Microbiology, 2010, 156: 3754-3761.
[15] Lin Z, Li WH. Expansion of hexose transporter genes was associated with the evolution of aerobic fermentation in yeasts[J]. Molecular Biology and Evolution, 2011, 28: 131-142.
[16] Bernard EA. Hexokinases from yeast[J]. Methods Enzymology, 1975, 42: 6-20.
[17] Berthels NJ, Cordero Otero RR, Bauer FF, et al. Correlation between glucose/fructose discrepancy and hexokinase kinetic properties in different Saccharomyces cerevisiae wine yeast strains[J]. Applied Microbiology & Biotechnology, 2008, 77: 1083-1091.
[18] Díaz-Campillo M, Urtíz N, Soto O, et al. Effect of glucose concentration on the rate of fructose consumption in native strains isolated from the fermentation of Agave duranguensis[J]. World Journal of Microbiology and Biotechnology, 2012, 28: 3387-3391.
[19] Varela C, Cardenas J, Melo F, Agosin E. Quantitative analysis of wine yeast gene expression profiles under winemaking conditions[J]. Yeast, 2005, 22: 369-383.
[20] You KM, Rosenfield CL, Knipple DC. Ethanol tolerance in the yeast Saccharomyces cerevisiae is dependent on cellular oleic acid content[J]. Applied and Environmental Microbiology, 2003, 69: 1499-1503.
[21] Zinnai A, Venturi F, Sanmartin C, et al. Kinetics of d-glucose and d-fructose conversion during the alcoholic fermentation promoted by Saccharomyces cerevisiae[J]. Journal of Bioscience and Bioengineering, 2013, 115: 43-49.
[22] Santos J, Sousa MJ, Cardoso H, et al. Ethanol tolerance of sugar transport, and the rectification of stuck wine fermentations[J]. Microbiology, 2008, 154(2)：422-430.
[23] Mateo, JJ, Jiménez M, Pastor A. Yeast starter cultures affecting wine fermentation and volatiles[J]. Food Research International, 2001, 34: 307-314.
[24] Tronchoni J, Gamero A, Arroyo-Lopez FN, et al. Differences in the glucose and fructose consumption profiles in diverse Saccharomyces wine species and their hybrids during grape juice fermentation[J]. International Journal of Food Microbiology, 2009, 1: 134: 237.

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