印度梨形孢定殖油菜对萝卜蚜选择油菜寄主的影响

doi:10.13560/j.cnki.biotech.bull.1985.2014.12.022

摘要/Abstract

摘要： 旨在研究印度梨形孢(Piriformospora indica)定殖油菜(Brassica napus L.)对萝卜蚜(Lipaphis erysimi(Kaltenbach)) 选择寄主油菜的影响，分别在大棚拟自然环境和实验室人工环境下进行蚜虫双向选择试验，发现萝卜蚜在有印度梨形孢定殖油菜上的数量显著低于无印度梨形孢定殖的油菜。利用顶空动态吸附装置收集不同处理油菜的挥发性物质，通过气相色谱-质谱联用仪(GC-MS)分析收集到的两者挥发物在种类和含量上的差异。结果表明，有印度梨形孢定殖的油菜与无印度梨形孢定殖的油菜，挥发物的种类没有变化，而挥发物的总量、α-pinene、terpinolene、camphor、β-thujene、cedrene 以及β-caryophyllene，在单位时间内有印度梨形孢定殖的油菜释放的量显著高于无印度梨形孢定殖的油菜。通过四扇区嗅觉仪测定萝卜蚜对挥发物的生物反应，发现 α-pinene 对萝卜蚜有显著的吸引作用，而 camphor 以及 camphor 与 α-pinene 浓度比为 2∶1 混合物对萝卜蚜具有显著的驱避作用，其余物质则没有作用。

关键词: 印度梨形孢, 萝卜蚜, 寄主选择, 挥发物

Abstract: In order to confirm the influence of Piriformospora indica on host plant selection of L. erysimi. Aphid dual-choice assay was taken under greenhouse and artificial control environment and the amounts of aphids on the P. indica-colonized B. napus was significantly less than the P. indica-free B. napus. Volatiles emitted by plants collected by dynamic headspace systems, the quality and quantitative differences of volatiles emitted by B. napus were analyzed through GC-MS. The result indicated that P. indica inoculated B. napus and P. indica-free B. napus had the same volatiles, but the quantity of total volatiles, α-pinene, terpinolene, camphor, β-thujene, cedrene and β-caryophyllene emitted by B. napus with P. indica were significantly higher than P. indica-free B. napus in unit time. Through the four-sector olfactomete method, we confirmed that the α-pinene significantly attracted the L. erysimi, while the camphor significantly repelled the L. erysimi. And the mixture of camphor and α-pinene(2∶1)was also significantly repelled the L. erysimi. Terpinolene, β-thujene, cedrene and β-caryophyllene had no effect on the L. erysimi.

Key words: Piriformospora indica, Lipaphis erysimi, Host plant selection, Volatile

刘金华 ,王婷 ,高其康. 印度梨形孢定殖油菜对萝卜蚜选择油菜寄主的影响[J]. 生物技术通报, 2014, 0(12): 133-140.

Liu Jinhua, Wang Ting, Gao Qikang. Influence of Piriformospora indica on Host Plant Selection by Aphid Lipaphis erysimi(Kaltenbach)[J]. Biotechnology Bulletin, 2014, 0(12): 133-140.

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