蛋白质胶内酶切技术研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2015.01.009

摘要/Abstract

摘要： 胶内酶切是蛋白质组研究中衔接电泳分离和质谱鉴定的重要环节，对最终的蛋白质定性和定量分析结果有显著的影响。该技术自1992年初步建立以来，一直处于不断完善中，出现了种类繁多的改进方案。为了更有效地利用胶内酶切技术，从凝胶脱色、杂质去除、蛋白酶切、肽段提取4个方面归纳整理了近年来蛋白质胶内酶切技术的主要研究进展。

关键词: 蛋白质胶内酶切, 样品制备, 质谱分析, 电泳分离, 技术进展

Abstract: In-gel digestion is the important step that connects electrophoresis separation and mass spectrometry identification in proteome research, and can significantlyaffect the results of qualitative and quantitative analysis of proteins. The technology was firstly proposed in 1992, and from then on, many progresses have been made and various improvement plans have been developed. In order to more effectively use the technology of in-gel digestion, the progress on the aspects of gel decoloration, impurity removal, protein digestion and peptides extraction were reviewed from the literature.

Key words: in-gel protein digestion, sample preparation, mass spectrometry, electrophoretic separation, technical progress

姜超,张学文,潘映红. 蛋白质胶内酶切技术研究进展[J]. 生物技术通报, 2015, 31(1): 61-66.

Jiang Chao, Zhang Xuewen, Pan Yinghong. Research Progress on Technology ofIn-gelProteinDigestion[J]. Biotechnology Bulletin, 2015, 31(1): 61-66.

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