豚鼠和BALB/c小鼠对SEC2超抗原作用敏感性的比较

doi:10.13560/j.cnki.biotech.bull.1985.2015.09.032

生物技术通报 ›› 2015, Vol. 31 ›› Issue (9): 224-231.doi: 10.13560/j.cnki.biotech.bull.1985.2015.09.032

豚鼠和BALB/c小鼠对SEC2超抗原作用敏感性的比较

孟北乾^1.2, 张惠文², 张国俊^1.2, 徐明恺², 李旭², 张成刚²

(1.中国科学院大学，北京 100049；2.中国科学院沈阳应用生态研究所，沈阳 110016)

收稿日期:2015-01-11 出版日期:2015-09-15 发布日期:2015-09-16
作者简介:孟北乾，男，硕士研究生，研究方向：口蹄疫疫苗佐剂的研究；E-mail：13995111458@163.com
基金资助:
十二五国家科技重大专项重大新药创制项目(2012ZX09102301-013)，沈阳市科技计划项目(F11-264-1-11，F12-152-9-00)

Comparison of the Superantigen Sensitivity on Staphylococcal Enterotoxins C2 in Guinea Pigs and BALB/c Mice

Meng Beiqian^1，2, Zhang Huiwen², Zhang Guojun^1，2, Xu Mingkai², Li Xu², Zhang Chenggong¹

(1. University of Chinese Academy of Sciences，Beijing 100049；2. Institute of Applied Ecology，Chinese Academy of Sciences，Shenyang 110016)

Received:2015-01-11 Published:2015-09-15 Online:2015-09-16

摘要/Abstract

摘要： 比较分析豚鼠和BALB/c小鼠对金黄色葡萄球菌肠毒素C2(Staphylococcus enterotoxin C2，SEC2)超抗原作用的敏感性差异，确定更适合用于SEC2超抗原作用研究的模式动物。体外试验，以梯度浓度SEC2刺激豚鼠和BALB/c小鼠的外周血单个核细胞(Peripheral blood mononuclear cell，PBMC)和脾淋巴细胞，MTS染色法检测其增殖；体内实验，SEC2腹腔注射豚鼠和BALB/c小鼠，每隔3 d检测豚鼠和小鼠的体重、体温变化情况；每隔7 d采血，ELISA法检测血清中IL-4、IFN-γ、IL-2和TNF-α分泌水平。不同浓度的SEC2均能刺激小鼠的PBMC和脾淋巴细胞显著增殖(P<0.05)，而对豚鼠的PBMC和脾淋巴细胞无效。腹腔注射后，SEC2组和对照组豚鼠和小鼠的体温均无显著性变化(P>0.05)；豚鼠SEC2组体重在给药后期显著低于对照组(P<0.05)，小鼠的SEC2组体重在给药后第3-30天均显著低于对照组(P<0.05)，此后恢复到正常水平(P>0.05)；给药后各时间点，小鼠的SEC2组血清中细胞因子IL-4、IFN-γ、IL-2和TNF-α的分泌水平均显著高于对照组(P<0.05)，而豚鼠的SEC2组血清中细胞因子分泌水平与对照组无显著性差异(P>0.05)。与豚鼠相比，BALB/c小鼠对SEC2超抗原活性的敏感程度更高，且表现出很好的剂量和时间效应，适合作为模式动物用于SEC2的超抗原活性研究。

关键词: 豚鼠, 小鼠, 金黄色葡萄球菌肠毒素C2

Abstract: This work is to analyze and compare the superantigen sensitivity on staphylococcal enterotoxins C2(SEC2)in guinea pigs and BALB/c mice, and determine the model animal for the researches of superantigen sensitivity on SEC2. In vitro, peripheral blood mononuclear cell(PBMC)and splenocytes from guinea pigs and BALB/c mice were respectively stimulated with SEC2 at gradient concentration, and the cell proliferation was tested by MTS assay；In vivo, the changes of temperature and weight of guinea pigs and mice were measured at interval of 3 d after the intraperitoneal injection of SEC2. Meanwhile, the serums were collected at interval of 7 d to detect the levels of IL-4, IFN-γ, IL-2 and TNF-α by ELISA. SEC2 of different concentrations significantly stimulated the proliferation of PBMC and splenocytes from BALB/c mouse(P < 0.05), but not so from guinea pigs(P > 0.05). After intraperitoneal injection of SEC2, there was no significant difference in body temperature of both guinea pigs and mice(P > 0.05)compared to the control. In the later phase of the experiment, the body weights of guinea pigs in SEC2 group were significantly lower than those in control group(P < 0.05). And the body weights of mice in SEC2 group were also significantly lower than those in control group during day 3 to 30, but recovered to normal level at the end of the experiment(P > 0.05). The cytokines IL-4, IFN-γ, IL-2 and TNF-α in serum of mice of SEC2 group were significantly elevated compared with in control group(P < 0.05). However, there was no significant difference in guinea pigs administrated with SEC2 and physiological saline(P > 0.05). In conclusion, comparing with guinea pigs, BALB/c mice are more sensitive to the stimulation of SEC2 with promising dose-dependent and time-dependent manner. Therefore, BALB/c mouse is a suitable model animal for the researches of superantigen activity.

Key words: guinea pig, mouse, staphylococcal enterotoxins C2

孟北乾, 张惠文, 张国俊, 徐明恺, 李旭, 张成刚. 豚鼠和BALB/c小鼠对SEC2超抗原作用敏感性的比较[J]. 生物技术通报, 2015, 31(9): 224-231.

Meng Beiqian, Zhang Huiwen, Zhang Guojun, Xu Mingkai, Li Xu, Zhang Chenggong. Comparison of the Superantigen Sensitivity on Staphylococcal Enterotoxins C2 in Guinea Pigs and BALB/c Mice[J]. Biotechnology Bulletin, 2015, 31(9): 224-231.

参考文献

[1]Fraser JD, Proft T. The bacterial superantigen and superantigen-like proteins[J]. Immunological Reviews, 2008(225)：226-243.
[2] Wang XG, Xu MK, Zhang HW, et al. Enhancement of superantigen activity and antitumor response of staphylococcal enterotoxin C2 by site-directed mutagenesis[J]. Cancer Immunol Immunother, 2009(58)：677-686.
[3] Liu YL, Xu MK, Zhang HW, et al. SEC2-induced superantigen and antitumor activity is regulated through calcineurin[J]. Applied Microbiology and Biotechnology, 2013, 97(22)：9695-9703.
[4] Liu YL, Xu MK, Li X, et al. The construction of a bifunctional fusion protein consisting of SEC2 and EGFP[J]. Biotechnology and Applied Biochemistry, 2014, 61(5)：565-571.
[5] 李华, 陆丽, 刘金保, 等. 金黄色葡萄球菌肠毒素B对豚鼠哮喘模型Th1/Th2细胞因子的影响[J]. 免疫学杂志, 2006, 22(3)：139-141.
[6] 张国俊, 徐明恺, 孙健, 等. 增强型金黄色葡萄球菌肠毒素C2突变体及其超抗原活性[J]. 生物工程学报, 2013, 29(6)：803-813.
[7] 张国俊, 邹谨, 徐明恺, 等. 活性增强的SEC2截短突变蛋白的构建及其生物活性研究[J]. 生物技术, 2012, 22(6)：31-35.
[8] Tang XY, Sun R, Hong SL, et al. Repeated intranasal instillation with staphylococcal enterotoxin B induces nasal allergic inflammation in guinea pigs[J]. American Journal of Rhinology & Allergy, 2011, 25(3)：176-181.
[9] Xu MK, Wang XG, Cai YM, et al. An engineered superantigen SEC2 exhibits promising antitumor activity and low toxicity[J]. Cancer Immunology Immunotherapy, 2011, 60(5)：705-714.
[10] Li ZJ, Omoe K, Shinagawa K, Yagi J, et al. Interaction between superantigen and T-cell receptor Vbeta element determines levels of superantigen-dependent cell-mediated cytotoxicity of CD8(+)T cells in induction and effector phases[J]. Microbiol Immunol, 2009, 53(8)：451-459.
[11] Llewelyn M, Sriskandan S, Terrazzini N, et al. The TCR Vb signature of bacterial superantigens spreads with stimulus strength[J]. International Immunology, 2006, 18(10)：1433-1441.
[12] Ranasinghe C, Trivedi S, Danushka K, et al. IL-4 and IL-13 recep-tors：Roles in immunity and powerful vaccine adjuvants[J]. Cytokine & Growth Factor Reviews, 2014, 25(4)：437-442.
[13] Ogawa, Hiromi, Mukai K, Kawano Y, et al. Th2-inducing cytokines IL-4 and IL-33 synergistically elicit the expression of transmembrane TNF-a on macrophages through the autocrine action of IL-6[J]. Biochemical and Biophysical Research Communications, 2012, 420(1)：114-118.
[14] Sharma R, Fu SM, Ju ST. IL-2：A two-faced master regulator of autoimmunity[J]. Journal of Autoimmunity, 2011, 36(2)：91-97.
[15] Liao W, Lin JX, Leonard, et al. IL-2 family cytokines：new insights into the complex roles of IL-2 as a broad regulator of T helper cell differentiation[J]. Current Opinion in Immunology, 2011, 23(5)：598-604.
[16] Pullyblank AM, Guillou PJ, Monson JRT, et al. Interleukin-1 and tumor-necrosis-factor-alpha may be responsible for the lytic mechanism during antitumor antibody-dependent cell-mediated cytotoxicity[J]. British Journal of Cancer, 1995, 72(3)：601-606.
[17] Papageorgion AC, Acharya KR, Shapiro R, et al. Crystal-structure of the superantigen enterotoxin c2 from Staphylococcus aureus reveals a zinc-binding site[J]. Structure, 1995, 3(8)：769-779.

豚鼠和BALB/c小鼠对SEC2超抗原作用敏感性的比较

Comparison of the Superantigen Sensitivity on Staphylococcal Enterotoxins C2 in Guinea Pigs and BALB/c Mice

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	丁亚群, 丁宁, 谢深民, 黄梦娜, 张昱, 张勤, 姜力. Vps28基因敲除小鼠模型的构建及其对泌乳和免疫性状影响的研究[J]. 生物技术通报, 2022, 38(3): 164-172.
[2]	谢绍怡, 蒋璐蔓, 杨晓峰, 张仙玉, 吴珍芳, 李紫聪. 切割小鼠X染色体的CRISPR/Cas9系统表达载体的构建及验证[J]. 生物技术通报, 2021, 37(5): 67-75.
[3]	张静, 熊燕, 华永琳, 郭玉, 熊显荣, 字向东, 李键. 小鼠骨骼肌纤维类型定量PCR内参基因的筛选[J]. 生物技术通报, 2021, 37(2): 71-79.
[4]	王棋文, 李盼, 潘翠云, 韩芬霞. 乙二醇对体内外源基因表达的作用研究[J]. 生物技术通报, 2019, 35(4): 64-68.
[5]	张达秀, 贺双丽, 王倩, 蒲仕明, 吴琼. 青年和老年小鼠棕色脂肪来源间充质干细胞生物学特性比较[J]. 生物技术通报, 2019, 35(2): 137-142.
[6]	耿慧君, 邹伟, 崔惠敬, 李晓宇, 王丽丽, 徐永平. 基于转录组学的金黄色葡萄球菌噬菌体安全性评估[J]. 生物技术通报, 2019, 35(12): 64-75.
[7]	董维鹏,王君实,张少华,燕炯. CRISPR系统及其应用于小鼠的研究进展[J]. 生物技术通报, 2018, 34(5): 57-63.
[8]	王跃强,AvinashBhandoola. 小鼠LSK及DN原代细胞中基因编辑方法的建立[J]. 生物技术通报, 2018, 34(5): 64-70.
[9]	张雪,陈亮亮,戴红霞,张文胜,任文燕. 利用CRISPR/Cas9系统在小鼠胚胎干细胞中进行miR-22的功能研究[J]. 生物技术通报, 2018, 34(5): 71-79.
[10]	袁白银, 刘钟颖. EDU脉冲追踪小鼠心脏第二心场细胞迁移方法的优化[J]. 生物技术通报, 2018, 34(12): 84-89.
[11]	袁白银, 胡继盛, 刘钟颖, 黄霞. 敲除Wdr1抑制小鼠原代血管平滑肌细胞的迁移和增殖[J]. 生物技术通报, 2018, 34(12): 179-185.
[12]	赵颖华,孙薇. 肝癌转基因小鼠模型的应用研究进展[J]. 生物技术通报, 2015, 31(12): 56-62.
[13]	罗奕秋, 迟大明, 李思奇, 王菲, 李延森, 李春梅,. 热应激对小鼠十二指肠组织形态和Caspase-3表达的影响[J]. 生物技术通报, 2013, 0(12): 84-87.
[14]	李倩,崔龙波,赵振军. 不同温度下VC对小鼠卵母细胞排卵后老化的调控作用[J]. 生物技术通报, 2013, 0(10): 113-119.
[15]	张远,杨旬旬,吴风瑞,刘勇,丁彪,王彩红,黄继昌,李文雍. 小鼠Dnmt1启动子荧光素酶报告基因的构建及活性分析[J]. 生物技术通报, 2013, 0(10): 109-112.