微流控芯片在植物细胞研究中的应用进展

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.005

摘要/Abstract

摘要： 植物细胞的传统分析方法是将植物细胞在土壤或者琼脂平板上生长，然后在温室或植物生长室内观察植物的表型。这种方法耗时耗力，且结果分辨率比较低。微流控芯片具有微型化、体积小和高通量等特点，且可在微米水平精确控制植物细胞生长的微环境。因此，能够降低实验成本，缩短实验时间，并且可以达到单细胞水平的分析和鉴定。首先介绍了微流控芯片的加工材料和制备方法，总结了用于植物细胞研究的微流控芯片，重点阐述了近年来微流控芯片在植物根、花粉管、原生质体和细胞壁动力学等植物细胞研究中的应用进展，并展望了微流控芯片在植物细胞研究的应用前景。

关键词: 微流控芯片, 聚二甲基硅氧烷, 植物细胞, 应用进展

Abstract: Conventional methods of studying plant cells rely on growing plant cells in soil pots or agarose plates，followed by screening the plant phenotypes in traditional greenhouses and growth chambers. These methods usually need a large number of experiments，and suffer from low spatial resolution. While the microfluidic chips have many advantages，such as miniaturization，small volume consumption and high-throughput analysis，etc，moreover，it allows to precisely control the micro-environment of plant cells at micron-level. Therefore，it can reduce the cost and experimental time，and achieve in vitro single cell analysis and characterization. In this paper，the materials and manufacturing methods of the microfluidic chip were firstly introduced，then the recent microfluidic chips for studying plant cells were summarized，further mainly the application progress of microfluidic chips in plant root，pollen tube，protoplasts，and cell wall biomechanics were expounded，and finally the future research directions of microfluidic chips for plant cell were prospected.

Key words: microfluidic chips, polydimethylsiloxane(PDMS), plant cell, application progress

引用本文

王伟轩, 孙静弈, 刘伟娜, 厚凌宇, 玉王宁, 何湘伟, 谢响明. 微流控芯片在植物细胞研究中的应用进展[J]. 生物技术通报, 2016, 32(6): 19-29.

WANG Wei-xuan, SUN Jing-yi, LIU Wei-na , HOU Ling-yu, YU Wang-ning, HE Xiang-wei, XIE Xiang-ming. The Application Progress of Microfluidic Chips in Studying Plant Cells[J]. Biotechnology Bulletin, 2016, 32(6): 19-29.

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微流控芯片在植物细胞研究中的应用进展

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[1]	张曼, 张叶卓, 何其邹洪, 鄂一岚, 李晔. 植物细胞壁结构及成像技术研究进展[J]. 生物技术通报, 2023, 39(7): 113-122.
[2]	陆新华, 孙德权, 张秀梅. 介孔硅纳米粒作为植物细胞转基因载体的研究[J]. 生物技术通报, 2022, 38(7): 194-204.
[3]	强晓楠, 李鑫, 陈佳, 廖红东, 于峰. 拟南芥RALF多肽家族的功能多样性初步分析[J]. 生物技术通报, 2019, 35(1): 2-10.
[4]	公维丽,王禄山,张怀强. 植物细胞壁多糖合成酶系及真菌降解酶系[J]. 生物技术通报, 2015, 31(4): 149-165.
[5]	曾杰(邦哲);. 系统遗传学与生物技术-细胞分子生物系统的分析与合成[J]. , 2012, 0(10): 47-51.
[6]	蔡绍皙;郭志刚;. 微流控芯片分析技术在生化研究中的应用进展[J]. , 2010, 0(11): 68-71.
[7]	蔡绍皙;黎昌莉;. 微流控与细胞迁移技术的研究进展[J]. , 2010, 0(03): 72-75.
[8]	李睿;周金池;卢存福;. 拉曼光谱在生物学领域的应用[J]. , 2009, 0(12): 62-64.
[9]	许守明;杨蓓;. 拟南芥中绿色荧光蛋白技术的改进及其意义[J]. , 2009, 0(08): 37-37.
[10]	李玉梅;姚纪元;吴静;白秀娟;. PCR-SSCP技术的研究及应用进展[J]. , 2007, 0(06): 71-74.
[11]	李万云;李培夫;. 外源基因导入技术在主要农作物育种上的应用进展[J]. , 2006, 0(06): 76-80.
[12]	汪开治;. 新兴学科“工能离子组学”简介[J]. , 2006, 0(05): 130-130.
[13]	张晓勇;王海林;高向阳;林壁润;徐凤彩;. 植物细胞培养生产重组蛋白[J]. , 2005, 0(06): 43-45.
[14]	. 《生物技术通报》2005年总目次[J]. , 2005, 0(06): 102-105.
[15]	汪开治. 第14届欧洲植物生物学联合会学术研讨会将召开[J]. , 2004, 0(01): 30-30.