外源ABA及其抑制剂钨酸钠对木薯块根类胡萝卜素相关基因和蛋白的影响

doi:10.13560/j.cnki.biotech.bull.1985.2017-0349

生物技术通报 ›› 2017, Vol. 33 ›› Issue (11): 76-83.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0349

外源ABA及其抑制剂钨酸钠对木薯块根类胡萝卜素相关基因和蛋白的影响

邓昌哲^1,2, 安飞飞¹, 李开绵¹, 陈松笔¹

1. 中国热带农业科学院热带作物品种资源研究所农业部木薯种质资源保护与利用重点实验室,儋州 571737;
2. 海南大学农学院,海口 570228

收稿日期:2017-04-29 出版日期:2017-11-26 发布日期:2017-11-22
作者简介:邓昌哲,男,硕士研究生,研究方向：木薯分子育种;E-mail：344498529@qq.com
基金资助:
国家自然科学基金面上项目（31271776）

Effects of ABA and Its Synthesis Inhibitor Sodium Tungstate on Carotenoid Associated Genes and Enzymes of Cassava Tuber Root

DENG Chang-zhe^1,2, AN Fei-fei¹, LI Kai-mian¹, CHEN Song-bi¹

1. Tropical Crops Genetic resources Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava,Danzhou 571737;
2. Hainan University,Haikou 570228

Received:2017-04-29 Published:2017-11-26 Online:2017-11-22

摘要/Abstract

摘要： 旨在研究外源ABA及其抑制剂钨酸钠（Na₂WO₄）对木薯块根类胡萝卜素相关基因和蛋白的影响。以木薯品种华南9号（Manihot esculenta Crantz SC9）为材料,HPLC测定块根β-胡萝卜素;Western blot分析相关蛋白表达量;QPCR分析相关基因表达量。结果显示,外源ABA及钨酸钠能提高块根β-胡萝卜素的含量;其中外源ABA与钨酸钠都提高了类胡萝卜素合成途径基因PSY2与LCYB表达,并抑制降解相关的ZEP与NCED3基因的表达,从而能够在转录水平的变化上解释β-胡萝卜素含量的变化,且LCYB与ZEP在转录与翻译水平具有一致性;与质体相关PAP1基因和FBN1a在处理下上调表达在一定程度上增加了质体小球的数量;外源ABA及钨酸钠使活性氧（ROS）相关的超氧化物歧化酶（Fe-SOD和Cu/Zn-SOD）表达量升高保护了块根中类胡萝卜素的稳定。这三者共同作用,提高了块根β-胡萝卜素的含量。

关键词: 木薯块根, 类胡萝卜素相关基因和酶, 脱落酸, β-胡萝卜素

Abstract: This work aims to analyze the effect of the exogenous ABA and its inhibitor sodium tungstate（Na₂WO₄）on carotenoid associated genes and protein of cassava tuberous root. Manihot esculenta Crantz SC9 was chosen as research subject and the β-carotene of cassava tuber root was detected by HPLC,and Western blot was used to analyze the carotenoid associated protein expression and QPCR to analyze the genes expression. The results showed that exogenous ABA and sodium tungstate increased the β-carotene content in the tuber root. Both exogenous ABA and sodium tungstate increased carotenoid biosynthesis key genes PSY2 and LCYB expressions,meanwhile they restrained the degradation genes ZEP and NCED3expressions,which explained the change of β-carotene content at the transcription level. The LCYB and ZEP transcriptions were consistent with translations. Plastid associated gene PAP1 and enzyme FBN1a were up-regulated,which increased the number of plastidglobules. Exogenous ABA and sodium tungstate accelerated the reactive oxygen（ROS）related superoxide dismutase（Fe-SOD and Cu/Zn-SOD）expressions,keeping the carotenoid content stable. In conclusion,these three factors jointly increase the β-carotene content of tuber root.

Key words: cassava tuber root, carotenoid associated genes and enzymes, abscisic acid, β-carotene

邓昌哲, 安飞飞, 李开绵, 陈松笔. 外源ABA及其抑制剂钨酸钠对木薯块根类胡萝卜素相关基因和蛋白的影响[J]. 生物技术通报, 2017, 33(11): 76-83.

DENG Chang-zhe, AN Fei-fei, LI Kai-mian, CHEN Song-bi. Effects of ABA and Its Synthesis Inhibitor Sodium Tungstate on Carotenoid Associated Genes and Enzymes of Cassava Tuber Root[J]. Biotechnology Bulletin, 2017, 33(11): 76-83.

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外源ABA及其抑制剂钨酸钠对木薯块根类胡萝卜素相关基因和蛋白的影响

Effects of ABA and Its Synthesis Inhibitor Sodium Tungstate on Carotenoid Associated Genes and Enzymes of Cassava Tuber Root

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