生物技术通报 ›› 2018, Vol. 34 ›› Issue (4): 139-143.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0847

• 研究报告 • 上一篇    下一篇

酿酒酵母PMT1与PMT3双基因缺失对细胞寿命的影响

崔红晶, 周涛, 邱堃佩, 宋浩昌, 刘新光   

  1. 广东医科大学衰老研究所 广东省医学分子诊断重点实验室 东莞市衰老与抗衰老重点实验室,东莞 523808
  • 收稿日期:2017-10-13 出版日期:2018-04-20 发布日期:2018-05-04
  • 作者简介:崔红晶,女,博士,研究方向:细胞衰老的分子机制;E-mail:cjingjing79@126.com
  • 基金资助:
    国家自然科学基金项目(31701050,81671399),广东省医学科学技术研究基金项目(A2016257,B2014299),东莞市医疗卫生科技计划一般项目(2016105101289),东莞市国际科技合作(含港澳台)项目(201650812001)

Effects of PMT1 and PMT3 Double-gene Deficiency on the Lifespan of Saccharomyces cerevisiae

CUI Hong-jing, ZHOU Tao, QIU Kun-pei, SONG Hao-chang, LIU Xin-guang   

  1. Institute of Aging Research,Guangdong Medical University; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics,Guangdong Medical University; Aging and Anti-aging Key Laboratory of Dongguan,Guangdong Medical University,Dongguan 523808
  • Received:2017-10-13 Published:2018-04-20 Online:2018-05-04

摘要: 研究蛋白质O-甘露糖转移酶(Protein O-mannosyltransferase,PMT)家族中PMT3基因缺失,以及PMT1与PMT3双基因缺失对酵母细胞复制性寿命的影响。采用一步基因置换法构建PMT3基因缺失酵母菌株(pmt3Δ),基于基因同源重组的原理,构建PMT1和PMT3双基因缺失酵母菌株(pmt1Δpmt3Δ);显微镜下分离和计数酵母子细胞的数目,统计酵母细胞复制性寿命。与野生型酵母细胞的平均复制性寿命(26代)比较,pmt3Δ菌株(24代,P > 0.05)和pmt1Δpmt3Δ菌株(25代,P > 0.05)的平均寿命均无明显变化,差异无统计学意义。酵母PMT3单基因缺失,以及PMT1和PMT3双基因缺失均不影响细胞的复制性寿命。

关键词: 酿酒酵母, 细胞寿命, 出芽, 糖基化

Abstract: We herein aimed to explore the effects of the PMT3 of protein O-mannosyltransferase(PMT)single-gene deficiency,and PMT1 and PMT3 double-gene deficiency on the replicative lifespan of Saccharomyces cerevisiae. PMT3 deficiency strain(pmt3Δ)was constructed through polymerase chain reaction(PCR)-mediated one-step gene disruption,and PMT1 and PMT3 double-gene deficiency strain(pmt1Δpmt3Δ)was prepared based on homologous recombination. The replicative lifespan of yeast cells were counted by separating daughter cells from mother cells using a manual micromanipulator equipped with a fiber-optic needle under the microscope. The replicative lifespan of the pmt3Δ yeast strain(24 generations)and pmt1Δpmt3Δ strain(25 generations,P > 0.05)exhibited no significant difference,compared with that of wild-type yeast strain(26 generations)(P > 0.05),i.e.,there was no statistical significance among the differences. Conclusively,PMT3 single-gene deficiency and PMT1 and PMT3 double-gene deficiency exert no evident effects on the lifespan of yeast cells.

Key words: Saccharomyces cerevisiae, cell lifespan, budding, glycosylation