生物技术通报 ›› 2018, Vol. 34 ›› Issue (8): 87-92.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0382

• 研究报告 • 上一篇    下一篇

普通野生稻根特异启动子的克隆与鉴定

黄珂1, 黄格格1, 薛满德2, 龙艳2, 袁潜华1, 裴新梧2   

  1. 1. 海南大学热带农林学院,海口 570228;
    2. 农业部转基因安全评价重点实验室 中国农业科学院生物技术研究所,北京 100081
  • 收稿日期:2018-04-25 出版日期:2018-08-26 发布日期:2018-09-04
  • 作者简介:黄珂,女,硕士,研究方向:作物基因工程;E-mail:huangke199309@163.com
  • 基金资助:
    转基因重大专项(2016ZX08011-001),国家自然科学基金项目(31260153)

Cloning and Identification of a Promoter with Root Tissue-specific Expression in Common Wild Rice

HUANG Ke1, HUANG Ge-ge1, XUE Man-de2, LONG Yan2, YUAN Qian-hua1, PEI Xin-wu2   

  1. 1. College of Tropical Agriculture and Forestry,Hainan University,Haikou 570228;
    2. MOA Key Laboratory on Safety Assessment(Molecular)of Agri-GMO,Chinese Academy of Agricultural Sciences,Beijing 100081
  • Received:2018-04-25 Published:2018-08-26 Online:2018-09-04

摘要: 通过普通野生稻转录组文库筛选到一个普通野生稻(Oryza rufipogon Griff.)根特异表达基因,并克隆启动子序列,命名为OrRSGp,长度为896 bp,含有CAAT-box、TATA-box等主要功能元件,还有参与茉莉酸甲酯反应的响应元件,以及抗旱、抗逆等响应元件。将其与gus报告基因融合转化拟南芥,GUS组织化学染色与定量分析结果表明,OrRSGp调控gus基因在根部特异表达。

关键词: 普通野生稻, 启动子, 根特异表达

Abstract: Based on previous transcriptome data,a root-specific expression gene of common wild rice(Oryza rufipogon Griff.)was screened,and the promoter sequence was cloned and designated as OrRSGp. It was 896 bp in length and contained main functional elements such as CAAT-box and TATA-box,MeJA-responsiveness and some elements involved in defense and stress responsiveness. It was fused with the β-glucuronidas(gus)reporter gene and transformed into Arabidopsis thaliana. GUS histochemical staining and quantitative analysis showed that OrRSGp regulated gene gus specific expression in roots.

Key words: common wild rice, promoter, root tissue-specific expression