衰老进程中小鼠造血干/祖细胞特征性改变对老年免疫失衡的影响

doi:10.13560/j.cnki.biotech.bull.1985.2018-0149

生物技术通报 ›› 2018, Vol. 34 ›› Issue (8): 199-203.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0149

衰老进程中小鼠造血干/祖细胞特征性改变对老年免疫失衡的影响

张立杰¹, 李晓玉¹, 岑由飞¹, 周祖平^2,3, 蒲仕明^1,2,3

1. 广西师范大学生命科学学院,桂林 541004;
2. 广西高校干细胞与医药生物技术重点实验室,桂林 541004;
3. 广西师范大学生物医学研究中心,桂林 541004

收稿日期:2018-02-24 出版日期:2018-08-26 发布日期:2018-09-04
作者简介:张立杰,女,硕士研究生,研究方向：细胞生物学;E-mail：1259082834@qq.com
基金资助:
广西研究生教育创新计划项目（YCSW2017078）,广西师范大学青年基金项目（2016QN003）,广西师范大学教育发展基金项目（EDF2015004）,国家自然科学基金项目（31270949）

Effects of the Characteristic Changes of Mice Hematopoietic Stem/Progenitor Cells on the Aged Immune Imbalance in the Aging Process

ZHANG Li-jie¹, LI Xiao-yu¹, CEN You-fei¹, ZHOU Zu-ping^2,3, PU Shi-ming^1,2,3

1. College of Life Sciences,Guangxi Normal University,Guilin 541004;
2 Guangxi Universities Key Laboratory of Stem cell and Biopharmaceutical Technology,Guilin 541004;
3. Research Center for Biomedical Sciences Guangxi Normal University,Guilin 541004

Received:2018-02-24 Published:2018-08-26 Online:2018-09-04

摘要/Abstract

摘要： 为探索小鼠造血干/祖细胞（Hematopoietic stem/progenitor cells,HSPCs）在衰老过程中的年龄相关性变化,并揭示衰老进程中免疫“老化”的发生、发展规律。分别利用流式细胞术对不同年龄小鼠的骨髓来源的造血干细胞（Hematopoietic stem cells,HSCs）及其下游祖细胞进行了细胞丰度和增殖活性测定。结果显示,HSCs和MPP（Multipotent Progenitor Cells,多能祖细胞）、GMPs（Granuiocyte/macrophage progenitor cells,粒细胞/巨噬细胞祖细胞）在骨髓细胞中的丰度随着年龄的增加而显著升高,其进入分裂期（G1、G2/S/M）的细胞数也随之增加。此外,GMPs下游的髓源性抑制细胞（Myeloid-derived suppressor cells,MDSCs）积累。结果揭示了HSCs在衰老进程中被活化,促进细胞增殖并偏向髓系抑制细胞分化与积累,抑制机体免疫功能。本研究可能为理解衰老免疫下降提供参考。

关键词: 衰老, 造血干/祖细胞, 髓系扩增, 免疫抑制

Abstract: This work is to explore the age-related changes of hematopoietic stem/progenitor cells（HSPCs）in the aging process and to reveal the occurrence and development mechanisms of immune “aging” in the aging process. Flow cytometry was used to measure the cell abundance and proliferation activity of hematopoietic stem cells（HSCs）and its downstream progenitor cells. The results showed that the abundances of HSCs and multipotent progenitor cell（MPP）and granuiocyte/macrophage progenitor（GMPs）cells in bone marrow cells increased significantly with the rise of age,and the number of cells while entering the phase of division（G1,G2/S/M）also increased. In addition,the myeloid-derived suppressor cells（MDSCs）in the downstream cells of GMPs accumulated. In conclusion,the results revealed that HSCs were activated in the aging process,promoting cell proliferation and the differentiation and accumulation of the myeloid suppressor cells,and inhibiting the immune function of the body. This study may provide reference for understanding the decline of immunity in the aging process.

Key words: aging, hematopoietic stem/ progenitor cells, medullary amplification, immunosuppression

张立杰, 李晓玉, 岑由飞, 周祖平, 蒲仕明. 衰老进程中小鼠造血干/祖细胞特征性改变对老年免疫失衡的影响[J]. 生物技术通报, 2018, 34(8): 199-203.

ZHANG Li-jie, LI Xiao-yu, CEN You-fei, ZHOU Zu-ping, PU Shi-ming. Effects of the Characteristic Changes of Mice Hematopoietic Stem/Progenitor Cells on the Aged Immune Imbalance in the Aging Process[J]. Biotechnology Bulletin, 2018, 34(8): 199-203.

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衰老进程中小鼠造血干/祖细胞特征性改变对老年免疫失衡的影响

Effects of the Characteristic Changes of Mice Hematopoietic Stem/Progenitor Cells on the Aged Immune Imbalance in the Aging Process

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