不同年龄大鼠尿液代谢组学研究

doi:10.13560/j.cnki.biotech.bull.1985.2021-0331

摘要/Abstract

摘要：

旨在探究健康大鼠的尿液代谢物在生长、发育和衰老过程中的年龄相关性变化。采集大鼠出生后3周、5周、7周、9周、12周、56周和111周共7个年龄点的尿液样本,利用GC/TOF-MS平台进行代谢组学检测。结果显示,所有尿液样本在主成分分析与偏最小二乘法判别分析中出现组内聚类和组间分离。氨基酸类、有机酸类和碳水化合物类代谢物是大鼠尿液样本中的主要代谢物类型。尿液代谢物如4-羟基脯氨酸、L-赖氨酸和尿酸等随年龄增加逐渐下降,D-核糖、乌头酸和富马酸等在老年阶段水平升高,而烟酸和5-羟基吲哚等在老年阶段水平下降。以上结果揭示了年龄影响大鼠尿液代谢,多种尿液代谢物在生长、发育和衰老过程中出现年龄相关性变化。

关键词: 生长, 发育, 衰老, 代谢组学, 尿液代谢物

Abstract:

This work aims to explore the age-related changes of urine metabolites in healthy rats during growth,development and aging. Urine samples were collected at 3,5,7,9,12,56,and 111 weeks after birth,and the GC/TOF-MS platform was employed for metabolomics detection. The results showed that all urine samples appeared in-group clustering and between-group separation in principal component analysis and partial least square discriminant analysis. Amino acids,organic acids,and carbohydrates were the dominant metabolite types in the rat urine samples. The levels of urine metabolites such as 4-hydroxyproline,L-lysine,and uric acid gradually decreased with age. D-ribose,aconitic acid,and fumaric acid increased in old age,while nicotinic acid and 5-hydroxyindole decreased in old age. The above results revealed that age affected urine metabolism in rats,and a variety of urine metabolites appeared age-related changes during growth,development and aging.

Key words: growth, development, aging, metabolomics, urine metabolites

杨玉萍, 张霞, 王翀翀, 王晓艳. 不同年龄大鼠尿液代谢组学研究[J]. 生物技术通报, 2022, 38(2): 166-172.

YANG Yu-ping, ZHANG Xia, WANG Chong-chong, WANG Xiao-yan. Study on Urine Metabolomics in Rats of Different Ages[J]. Biotechnology Bulletin, 2022, 38(2): 166-172.

图/表 4

图1 大鼠尿液样本取样时间点和年龄分段示意图

Fig.1 Schematic diagram of rat urine sampling time points and age segments

图2 大鼠尿液代谢轮廓散点图 A：主成分分析（PCA）;B：偏最小二乘法判别分析（PLS-DA）

Fig.2 Scatter plots of rat urine metabolism profile A：Principal component analysis（PCA）. B：Partial least squares discriminant analysis（PLS-DA）

图3 不同年龄段尿液代谢物类型相对丰度图

Fig.3 Relative abundance of metabolite types in rat urine with different age segments

图4 所有尿液代谢物的差异倍数热图差异倍数值大于2的均以2表示,*P < 0.05,**P < 0.01,***P < 0.001

Fig.4 Fold change heatmaps of all urine metabolites The fold change values of >2 are represented by 2. *P < 0.05,**P < 0.01,***P < 0.001

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