恒温技术介导的功能核酸生物传感器研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2018-0574

摘要/Abstract

摘要： 随着社会不断发展,消费者对食品安全的关注度日渐上升,食品多样性日益增加,食品流通量日益增长,对食品安全检测技术的要求不断提升。功能核酸通过形成特定的空间结构,能够发挥除了储存遗传信息以外的多种功能,在检测领域起到重要作用。功能核酸生物传感器是一类利用功能核酸进行信号识别、信号放大或者信号输出的传感器,具有高灵敏度、高特异性、检测时间短、成本低等优势。为了避免对变温仪器设备的依赖,实现现场检测,恒温技术介导的功能核酸生物传感器发展迅速。相对于变温技术,恒温技术无需变温设备,有些在室温条件下即可进行,能够降低检测成本,在一定程度上缩短检测时间。根据恒温技术在功能核酸生物传感器中的功能不同,可以分为恒温介导的信号识别技术、信号放大技术和信号输出技术。就这3个方面对恒温技术介导的功能核酸生物传感器展开论述,并从理论层面、应用层面和学科交叉方面提出展望。

关键词: 恒温技术, 功能核酸, 生物传感器

Abstract: With the development of society,food diversity,and food circulation,consumers’ attention to food safety is increasing,which makes the demands of detection technologies for food safety risk factors rise. Functional nucleic acids play an important role in the field of detection by forming specific spatial structures and playing a variety of functions besides storing genetic information. Functional nucleic acid biosensor is based on functional nucleic acid to recognize target,to amplify signal or to put out signal with high sensitivity,high specificity,time-saving,and low cost. In order to avoid the dependence on temperature-changing equipment and to conduct on-site detection,functional nucleic acid biosensor mediated by the isothermal technology develops rapidly. Compared with temperature-changing technology,isothermal technology does not need temperature-changing equipment and some of them can be carried out at room temperature,which reduces the cost of detection and shortens the detection time to some extent. According to the function of isothermal technology in functional nucleic acid biosensor,it can be divided into isothermal-mediated signal recognition technology,signal amplification technology and signal output technology. Isothermal technologies in functional nucleic acid biosensors are introduced from above three aspects in this review and prospects are also summarized from these three aspects.

Key words: isothermal technology, functional nucleic acid, biosensor

张园, 肖冰, 田晶晶, 许文涛. 恒温技术介导的功能核酸生物传感器研究进展[J]. 生物技术通报, 2018, 34(9): 29-38.

ZHANG Yuan, XIAO Bing, TIAN Jing-jing, XU Wen-tao. Research Progress on Functional Nucleic Acid Biosensor Mediated by Isothermal Technology[J]. Biotechnology Bulletin, 2018, 34(9): 29-38.

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