APE1介导的功能核酸生物传感器研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2017-1015

摘要/Abstract

摘要： 脱嘌呤/脱嘧啶核酸内切酶1（Apurinic/apyrimidinic endonuclease 1,APE1）是一种广泛存在于生物体内、在碱基切除修复（Base excision repair,BER）过程中能够在碱基缺失位点（AP site）处识别并切割DNA的蛋白酶,其作用效率高且特异性强。同时,APE1在一些癌症细胞中的活性较正常细胞明显偏高,因此其自身也是一种癌症生物标志物。目前,通过在DNA上人工设计AP位点,利用APE1的切割能力生成理想的功能核酸链,并结合不同的信号输出及放大方式,研究者已经建立了一些APE1介导的电化学、荧光功能核酸生物传感技术,实现了对DNA糖基化酶等的酶活性的检测。另外,也有一些针对APE1自身活性的功能核酸生物传感技术被建立起来。综述了近年来APE1介导的功能核酸生物传感技术以及以APE1为靶物质的功能核酸和免疫生物传感技术的研究状况,讨论了与APE1相关的生物传感技术的意义及存在的问题,并对未来利用APE1实现更多靶物质的检测的发展趋势进行了展望,以期促进APE1成为一种功能核酸生物传感技术中常用的酶工具。

关键词: 碱基缺失位点, 脱嘌呤/脱嘧啶核酸内切酶1, 功能核酸, 生物传感器

Abstract: Apurinic/apyrimidinic Endonuclease 1（APE1）is an enzyme widely existing in living organisms and can effectively and specifically identify and cleave DNA in AP site in the process of base excision repair（BER）. Meanwhile,the activity of APE1 in some tumor cells is significantly higher than that in normal cells,thus it is also a kind of tumor biomarker. At present,by designing AP sites in DNA strand artificially and utilizing the activity of APE1 to produce ideal functional nucleic acids,combined with different methods of signal output and amplification,researchers have established some APE1 mediated electrochemical and fluorescent functional nucleic acids biosensing techniques,by which the detection for the activities of some enzymes such as DNA glycosylase can be conducted. In addition,some functional nucleic acids biosensing techniques for the detection of the activity of APE1 were also established. In this paper we review the research status of APE1 mediated functional nucleic acids biosensing techniques and other biosensing techniques for the detection of APE1,discuss the significances and problems of APE1-relevant biosensing techniques,and prospect development trends of APE1 mediated detecting techniques for the detection of more targets,aiming at promoting APE1 to become a common enzyme tool in functional nucleic acids biosensing techniques.

Key words: AP site, apurinic/apyrimidinic endonuclease 1, functional nucleic acids, biosensor

贺万崇, 黄昆仑, 许文涛. APE1介导的功能核酸生物传感器研究进展[J]. 生物技术通报, 2018, 34(9): 48-54.

HE Wan-chong, HUANG Kun-lun, XU Wen-tao. Research Advances on Apurinic/Apyrimidinic Endonuclease 1 Mediated Functional Nucleic Acids Biosensors[J]. Biotechnology Bulletin, 2018, 34(9): 48-54.

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