生物技术通报 ›› 2019, Vol. 35 ›› Issue (3): 31-38.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0844

• 研究报告 • 上一篇    下一篇

林木根际细菌JYZ-SD5的促生抗逆性能及种类鉴定

徐秀倩, 吴小芹, 吴天宇, 曾梦嫚   

  1. 1. 南京林业大学南方现代林业协同创新中心,南京林业大学林学院,南京 210037;
    2. 江苏省有害生物入侵预防与控制重点实验室,南京 210007
  • 收稿日期:2018-09-18 出版日期:2019-03-26 发布日期:2019-04-03
  • 作者简介:徐秀倩,女,硕士研究生,研究方向:森林保护;E-mail:xuxq1107xl@foxmail.com
  • 基金资助:
    国家重点研发计划(2017YFD0600104),江苏高校优势学科建设工程项目(PAPD)

Growth-promoting and Adverse-resistant Characteristics of JYZ-SD5,a Tree Rhizobacterium and Its Species Identification

XU Xiu-qian, WU Xiao-qin, WU Tian-yu, ZENG Meng-man   

  1. 1. Co-Innovation Center for Sustainable Forestry in Southern China,College of Forestry,Nanjing Forestry University,Nanjing 210037;
    2. Jiangsu Key Laboratory for Prevention and Management of Invasive Species,Nanjing 210007
  • Received:2018-09-18 Published:2019-03-26 Online:2019-04-03

摘要: 旨在探究林木根际细菌JYZ-SD5促生抗逆性能,以期揭示该菌在野外应用的价值。采用特定培养基定性检测该菌的固氮解磷解钾特性,测定该菌产IAA能力;平板对峙法检测菌株拮抗病原真菌活性;检测该菌重金属耐受性;将根际细菌JYZ-SD5施用于水杉盆栽幼苗,观测对水杉生长的影响;采用形态、生理生化测试及 16S rRNA 基因序列分析,对菌株JYZ-SD5进行种类鉴定。结果显示,菌株JYZ-SD5具有固氮、解磷、解钾的能力;不加色氨酸前提下产IAA量可达6.818 6 μg/mL;对链格孢(Alternaria sp.)、异色状拟盘多毛孢(Pestalotiopsis vesicolor)有一定拮抗活性,可产生蛋白酶和纤维素酶;对重金属Mn7+、Cr6+耐受性强,最高均可达400 mg/L;温室盆栽试验表明,根际细菌JYZ-SD5可提高水杉苗木叶绿素相对含量,对水杉株高和地径增长具有促进作用。结合形态学、生理生化和分子鉴定的方法初步确定该菌为拟蕈状芽孢杆菌(Bacillus paramycoides)。根际细菌JYZ-SD5具有良好的促生抗逆特性,具有成为新型生物菌肥的开发潜力和野外应用价值。

关键词: 根际细菌, 促生特性, 抗逆特性, 种类鉴定

Abstract: This work aims to explore the performance of promoting growth and stress resistance of JYZ-SD5,a tree rhizobacterium,and to reveal the value of this bacterium in the field. Specific culture medium was used to detect its ability of nitrogen fixation and decomposition of phosphorus and potassium,also to measure its capacity of producing IAA. Then plate confrontation was employed to check the antagonistic activity to pathogenic fungi,and growth was observed to determine its tolerance to heavy metals. Further,the tree rhizobacterium JYZ-SD5 was applied to the seedlings of Metasequoia glyptostroboides Hu et Cheng in plots to test its effects on the growth of the seedlings. Morphology observation,physiological and biochemical tests,and 16S rRNA gene sequence analysis were combined to identify the strain JYZ-SD5. Results showed that JYZ-SD5 had the ability of fixing nitrogen and decomposing phosphorus and potassium. The production of IAA without adding tryptophan reached 6.818 6 μg/mL. JYZ-SD5 presented the antagonistic activity to Alternaria sp and Pestalotiopsis vesicolor,and protease and cellulose were also detected. This strain also demonstrated strong tolerance to heavy metals Mn7+ and Cr6+,to the highest content of 400 mg/L. The greenhouse plot experiment revealed that JYZ-SD5 resulted in the increase of relative content of chlorophyll in the seedlings of M. glyptostroboides Hu et Cheng,and it promoted the growth and ground diameter of M.glyptostroboides Hu et Cheng. Finally,the rhizobacterium was identified as Bacillus paramycoides by morphological,physiological,biochemical,and molecular approaches. In sum,the rhizobacterium JYZ-SD5 shows favorable performance on promoting growth and stress resistance,and thus it has the potential to be a novel biological fertilizer and meets the requirement of applying it in field.

Key words: molecular identification, growth-promoting property, adverse-resistant characteristics, species identification