生物技术通报 ›› 2019, Vol. 35 ›› Issue (3): 24-30.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0770

• 研究报告 • 上一篇    下一篇

花生根际多功能固氮菌的分离及其耐盐碱特性研究

姜焕焕1,2, 祁佩时2, 王通1, 迟晓元1, 陈明娜1   

  1. 1. 山东省花生研究所,青岛 266100;
    2. 哈尔滨工业大学环境学院,哈尔滨 150001
  • 收稿日期:2018-09-05 出版日期:2019-03-26 发布日期:2019-04-03
  • 作者简介:姜焕焕,女,博士,研究方向:土壤生态学;E-mail:767360114@qq.com
  • 基金资助:
    国家自然科学基金项目(31701464),城市水资源与水环境国家重点实验室(2017DX15),2014年国家“万人计划”青年拔尖人才(W02070268),国家花生产业技术体系项目(CARS-13),山东省自然科学基金项目(ZR2014YL011,ZR2014YL012,ZR2017YL017),山东省农业科学院青年科研基金项目(2016YQN14),山东省农业科学院青年英才培养计划,山东省农业科学院农业科技创新工程(CXGC2016B02),青岛市应用研究专项青年专项(17-1-1-51-jch),山东省良种工程(2017LZGC003)

Screening of Multi-function Nitrogen-fixing Bacteria in Peanut Rhizosphere and Their Tolerances to Saline

JIANG Huan-huan1,2, QI Pei-shi2, WANG Tong1, CHI Xiao-yuan1, CHEN Ming-na1   

  1. 1. Shandong Peanut Research Institute,Qingdao 266100;
    2. Harbin Institute of Technology,Harbin 150000
  • Received:2018-09-05 Published:2019-03-26 Online:2019-04-03

摘要: 旨在获得具有固氮、溶磷及分泌IAA的多功能耐盐碱根际促生菌(PGPR),提高盐碱地花生产量。采用平板涂布法对盐碱地花生根际固氮菌进行了分离,结合16S rDNA序列鉴定和乙炔还原法测定固氮酶活。共获得22个固氮菌株,其固氮酶活性为125.82-346.32 nmol C2H4 /(h·mL)。所有菌株均具有产ACC脱氨酶及溶解难溶性磷源活性。其ACC脱氨酶活性为0.12-1.26 U/mg;溶解磷酸三钙量为1.45-53.58 mg/L,溶解磷酸铁量为1.45-8.27 mg/L,溶解磷酸铝量为1.1-22.82 mg/L。其中,10株具有分泌IAA的能力,分泌量在1.36-17.80 μg/mL,占供试菌株的55%。对这10株促生菌进行NaCl、pH耐受性测定,结果显示最大pH耐受力为9,最大NaCl耐受力为1.5 mol/L。这10菌株具有较强的耐盐碱性,具有在盐碱地中应用的潜力。

关键词: 盐碱地, 固氮酶活, 溶磷特性, ACC 脱氨酶, IAA, 耐盐碱

Abstract: This study aims to acquire the saline-tolerant root-promoting rhizobacteria(PGPR)with multi-function of fixing nitrogen,dissolving phosphorus and synthesizing the IAA for increasing the peanut yield in saline soil. Spread plate was used to isolate the peanut rhizosphere nitrogen-fixing bacteria in saline soil,16S rDNA was to identify the sequence,and acetylene reduction method to determine the nitrogenase activity. Total 22 nitrogen-fixing strains were obtained,and their nitrogenase activities were between 125.82-346.32 nmol C2H4/(h·mL). All strains had the activities of ACC deaminase and dissolving insoluble phosphorus source;their ACC deaminase activity were among 0.12-1.26 U/mg,the amount of dissolving tricalcium phosphate was between 1.45-53.58 mg/L,the that of dissolving ferric phosphate was between 1.45-8.27 mg/L,and that of dissolving aluminum phosphate was between 1.1-22.82 mg/L. Ten strains of them secreted IAA with the amount ranging in 1.36-1.36 g/mL,accounting for the 55% of the total strains. The ten strains were also tested for their survival abilities in different concentrations of NaCl and pH,results showed that the maximum tolerance to pH was 9 and the maximum tolerance to NaCl was 1.5 mol/L. In conclusion,the 10 strains demonstrate strong tolerance to salinity and showed their potential of applying in saline soil.

Key words: saline soil, nitrogenase activity, P-releasing, ACC deaminase, IAA, tolerance to salinity