生物技术通报 ›› 2019, Vol. 35 ›› Issue (5): 35-41.doi: 10.13560/j.cnki.biotech.bull.1985.2018-1102

• 高粱专题 • 上一篇    下一篇

甜高粱FAD7基因的功能分析

宋玉双, 隋娜   

  1. 山东师范大学生命科学学院,济南 250014
  • 收稿日期:2018-12-27 出版日期:2019-05-26 发布日期:2019-05-23
  • 作者简介:宋玉双,女,硕士研究生,研究方向:植物逆境生理与分子生物学;E-mail:15027620683@163.com
  • 基金资助:
    国家重点研发计划(2018YFD1000700,2018YFD1000704),国家自然科学基金项目(31871538)

Functional Analysis of FAD7 Gene in Sweet Sorghum

SONG Yu-shuang, SUI Na   

  1. Shandong Normal University,School of Life Sciences,Jinan 250014
  • Received:2018-12-27 Published:2019-05-26 Online:2019-05-23

摘要: 从甜高粱自交系M81-E中分离并克隆出ω-3脂肪酸去饱和酶FAD7(Fatty acid desaturase)基因,然后构建表达载体侵染拟南芥获得SbFAD7过表达拟南芥的纯合植株,通过测定和分析低温处理条件下野生型和过表达植株的Fo、Fv/Fm、电导率、MDA、超氧阴离子自由基和过氧化氢含量等指标,初步说明该基因在提高植物抗冷性方面具有重要的作用。

关键词: 甜高粱, 耐冷性, ω-3脂肪酸去饱和酶

Abstract: Here firstly we isolated and cloned ω-3 fatty acid desaturase(FAD7)gene from sweet sorghum inbred line M81-E. Then we constructed an expression vector to infect Arabidopsis thaliana and obtained the homozygous plants with SbFAD7overexpression. It was preliminarily indicated that the gene played an important role in increasing the cold resistance of plant by measuring and analyzing the contents of Fo,Fv/Fm,electrical conductivity,MDA,superoxide anion radical and hydrogen peroxide content in wild and overexpressed plants under low temperature treatment.

Key words: sweet sorghum, cold resistance, ω-3 fatty acid desaturase;