高粱A1型细胞质雄性不育系与保持系线粒体基因组分析比较

doi:10.13560/j.cnki.biotech.bull.1985.2019-0089

生物技术通报 ›› 2019, Vol. 35 ›› Issue (5): 42-47.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0089

高粱A1型细胞质雄性不育系与保持系线粒体基因组分析比较

王平¹, 丛玲¹, 王春语¹, 朱振兴¹, A Ashok Kumar², 张丽霞¹, 陆晓春¹

1.辽宁省农业科学院高粱研究所,沈阳 110161;
2.国际热带半干旱地区作物研究所,帕坦奇鲁印度502324

收稿日期:2019-01-23 出版日期:2019-05-26 发布日期:2019-05-23
作者简介:王平,男,博士研究生,研究方向：高粱分子育种技术研发,E-mail：pingw-556@163.com;丛玲同为本文第一作者
基金资助:
国家重点研发计划（2018YFD1000701,2018YFD1000700）,国家自然科学基金项目（31500203,31501241）,国家现代农业产业技术体系建设项目（CARS-06-13.5-A3）

Comparison of Mitochondrial Genome Between A1 Cytoplasmic Male Sterile Line and Maintainer Line of Sorghum bicolor

WANG Ping¹, CONG Ling¹, WANG Chun-yu¹, ZHU Zhen-xing¹, A Ashok KUMAR², ZHANG Li-xia¹, LU Xiao-chun¹

1.Sorghum Research Institute,Liaoning Academy of Agricultural Sciences,Shenyang 110161;
2.International Crops Research Institute for the Semi-Arid Tropics,Patancheru India 502324

Received:2019-01-23 Published:2019-05-26 Online:2019-05-23

摘要/Abstract

摘要： 线粒体基因组易位是导致作物细胞质雄性不育（Cytoplasmic male sterility,CMS）性状产生的重要遗传机制。比较高粱A1型细胞质雄性不育系与保持系线粒体基因组,寻找易位区为克隆高粱A1型细胞质雄性不育相关基因奠定基础。以高粱A1型细胞质雄性不育系Tx623A和其保持系Tx623B为试验材料,采用二代Illumina Hiseq结合三代PacBio测序技术,对2个样品的线粒体基因组进行组装,比较和分析不育系和保持系基因组结构和基因差异。高粱Tx623A和Tx623B线粒体基因组大小分别为449 727 bp和452 772 bp,预测编码开放阅读框（Open reading frame,ORFs）分别为147和145个,且两基因组特有基因分别为8个和6个。两线粒体基因组共线性比较分析,发现存在一个57 kb的基因组片段易位的结构变异（Structural variation,SV）区域,该易位区可能与A1型细胞质雄性不育有关。Tx623A和Tx623B线粒体基因组中易位区为高粱A1型细胞质雄性不育基因克隆提供了基因组信息。

关键词: 高粱, 不育系, 保持系, 线粒体基因组, 细胞质雄性不育

Abstract: Mitochondrial genomic translocation is an important genetic mechanism for cytoplasmic male sterility（CMS）in crops. The main purpose of this study is to explore the translocation region of mitochondrial genomes by comparing the mitochondrial genome between A1 cytoplasmic male sterile line and maintainer line of Sorghum bicolor,and to lay a foundation for cloning related genes of sorghum A1 cytoplasmic male sterility. A1 cytoplasmic male sterile line Tx623A and its maintainer line Tx623B of sorghum were used in this experiment. Their mitochondrial genome sequencing data from Illumina Hiseq（next generation sequencing）and PacBio seq（third generation sequencing）were assembled,and then,the differences of genome structures and genes between the two lines were compared and analyzed. The results showed that the mitochondrial genome sizes of Tx623A and Tx623B were 449 727 bp and 452 772 bp,respectively. The predicted open reading frames（ORFs）were 147 and 145,among which the number of the specific genes for Tx623A were 8 and 6 for Tx623B. Importantly,a 57 kb genome structural variation（SV）region was found after comparing the two mitochondrial genomes,and which may be related to A1 cytoplasmic male sterility. Collectively,our results provide genomic information for cloning related genes of sorghum A1 cytoplasmic male sterility.

Key words: Sorghum bicolor, sterile line, maintainer line, mitochondrial genome, cytoplasmic male sterility

王平, 丛玲, 王春语, 朱振兴, AAshokKumar, 张丽霞, 陆晓春. 高粱A1型细胞质雄性不育系与保持系线粒体基因组分析比较[J]. 生物技术通报, 2019, 35(5): 42-47.

WANG Ping, CONG Ling, WANG Chun-yu, ZHU Zhen-xing, A Ashok KUMAR, ZHANG Li-xia, LU Xiao-chun. Comparison of Mitochondrial Genome Between A1 Cytoplasmic Male Sterile Line and Maintainer Line of Sorghum bicolor[J]. Biotechnology Bulletin, 2019, 35(5): 42-47.

参考文献

[1] Tang HW, Xie YY, Liu YG, et al.Advances in understanding the molecular mechanisms of cytoplasmic male sterility and restoration in rice[J]. Plant Report, 2017, 30(4):179-184.
[2] Luo DP, Xu H, Liu ZL, et al.A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice[J]. Nat Genet, 2013, 45(5):573-577.
[3] Charlesworth D.Origins of rice cytoplasmic male sterility genes[J]. Cell Res, 2017, 27(1):3-4.
[4] Wang X, Guan Z, Gong Z, et al.Crystal structure of WA352 provides insight into cytoplasmic male sterility in rice[J]. Biochem Biophys Res Commun, 2018, 501(4):898-904.
[5] Wang ZH, Zou YJ, Li XY, et al.Cytoplasmic male sterility of rice with boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing[J]. The Plant Cell, 2006, 18(3):676-687.
[6] Kazama T, Itabashi E, Fujii S, et al.Mitochondrial ORF79 levels determine pollen abortion in cytoplasmic male sterile rice[J]. Plant J, 2016, 85(6):707-716.
[7] Wang K, Gao F, Ji YX, et al.ORFH79 impairs mitochondrial function via interaction with a subunit of electron transport chain complex III in Honglian cytoplasmic male sterile rice[J]. New Phytol, 2013, 198(2):408-418.
[8] Xie HW, Peng XJ, Qian MJ, et al.The chimeric mitochondrial gene orf182 causes non-pollen-type abortion in Dongxiang cytoplasmic male-sterile rice[J]. Plant J, 2018, 95(4):715-726.
[9] Yamamoto MP, Shinada H, Onodera Y, et al. A male sterility-associated mitochondrial protein in wild beets causes pollen disruption in transgenic plants[J].2008, Plant [J], 54:1027-1036.
[10] Kim DH, Kang JG, Kim BD.Isolation and characterization of the cytoplasmic male sterility associated orf456 gene of chili pepper(Capsicum annuum L.)[J]. Plant Mol Biol, 2007, 63:519-532.
[11] Yang J, Liu X, Yang X, et al.Mitochondrially-targeted expression of a cytoplasmic male sterility-associated orf220 gene causes male sterility in Brassica juncea[J]. BMC Plant Biol, 2010, 10:231-240.
[12] Jing B, Heng S, Tong D, et al.A male sterility-associated cytotoxic protein ORF288 in Brassica juncea causes aborted pollen development[J]. J Exp Bot, 2012, 63:1285-1295.
[13] Stephens JC, Holland RF.Cytoplasmic male sterility for hybrid sorghum seed production[J]. Agron J, 1954, 46:20-23.
[14] 卢庆善, 孙毅. 杂交高粱遗传改良[M]. 北京:中国农业科学技术出版社, 2005.
[15] Kante M, Rattunde HFW, Nébié B, et al.QTL mapping and validation of fertility restoration in West African sorghum A1 cytoplasm and identification of a potential causative mutation for Rf2[J]. Theor Appl Genet, 2018, 131(11):2397-2412.
[16] 张福耀, 牛天堂, 韦耀明, 等. 高粱非买罗细胞质A₂, A₃, A₄, A₅, A₆, 9E雄性不育系研究[J]. 山西农业科学, 1996, 24(3):3-6.
[17] 侯荷亭, 侯丽娟, 仪治本, 等. 高粱新胞质雄性不育遗传机理及三系配套的研究[J]. 华北农学报, 2001, 16(2):16-19.
[18] Tang HV, Pring DR, Shaw LC, et al.Transcript processing internal to a mitochondrial open reading frame is correlated with fertility restoration in male-sterile sorghum[J]. Plant J, 1996, 10:123-133.
[19] Jordan DR, Mace ES, Henzell RG, et al.Molecular mapping and candidate gene identification of the Rf2 gene for pollen fertility restoration in sorghum[Sorghum bicolor(L.)Moench][J]. Theor Appl Genet, 2010, 120:1279-1287.
[20] Jordan DR, Klein RR, Sakrewski KG, et al.Mapping and characterization of Rf5:a new gene conditioning pollen fertility restoration in A1 and A2 cytoplasm in sorghum[Sorghum bicolor(L.)Moench][J]. Theor Appl Genet, 2011, 123:383-396.
[21] Fujii S, Kazama T, Yamada M, et al.Discovery of global genomic re-organization based on comparison of two newly sequenced rice mitochondrial genomes with cytoplasmic male sterility-related genes[J]. BMC Genomics, 2010, 11:209-223.
[22] Clifton SW, Minx P, Fauron CM, et al.Sequence and comparative analysis of the maize NB mitochondrial genome[J]. Plant Physiol, 2004, 136(3):3486-3503.
[23] Kim YJ, Zhang DB.Molecular control of male fertility for crop hybrid breeding[J]. Trends Plant Sci, 2018, 23(1):53-65.
[24] Chen LT, Liu YG.Male sterility and fertility restoration in crops[J]. Annu Rev Plant Biol, 2014, 65:579-606.

高粱A1型细胞质雄性不育系与保持系线粒体基因组分析比较

Comparison of Mitochondrial Genome Between A1 Cytoplasmic Male Sterile Line and Maintainer Line of Sorghum bicolor

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	方澜, 黎妍妍, 江健伟, 成胜, 孙正祥, 周燚. 盘龙参内生真菌胞内细菌7-2H的分离鉴定和促生特性研究[J]. 生物技术通报, 2023, 39(8): 272-282.
[2]	范昕琦, 王海燕, 陈静, 张晓娟, 郭琦, 梁笃, 周福平, 聂萌恩, 张一中, 柳青山. EMS诱变对高粱成苗及M₁主要农艺性状的影响[J]. 生物技术通报, 2023, 39(7): 173-184.
[3]	徐建霞, 丁延庆, 冯周, 曹宁, 程斌, 高旭, 邹桂花, 张立异. 基于Super-GBS的高粱株高和节间数QTL定位[J]. 生物技术通报, 2023, 39(7): 185-194.
[4]	王春语, 李政君, 王平, 张丽霞. 高粱表皮蜡质缺失突变体sb1抗旱生理生化分析[J]. 生物技术通报, 2023, 39(5): 160-167.
[5]	曲春娟, 朱悦, 江晨, 曲明静, 王向誉, 李晓. 铜绿丽金龟线粒体全基因组及其系统发育分析[J]. 生物技术通报, 2023, 39(2): 263-273.
[6]	周诗晨, 仪治本, 王馨翊, 杨晓颖, 孙丽娜, 栾维江, 梁闪闪. 高粱双粒突变体Dgs的遗传分析与基因定位[J]. 生物技术通报, 2022, 38(7): 171-177.
[7]	杨益善, 孙平勇, 余木兰. 水稻不育系稻粒黑粉病抗性QTL的定位[J]. 生物技术通报, 2022, 38(3): 16-21.
[8]	薛清, 杜虹锐, 薛会英, 王译浩, 王暄, 李红梅. 苜蓿滑刃线虫线粒体基因组及其系统发育研究[J]. 生物技术通报, 2021, 37(7): 98-106.
[9]	江佰阳, 白文斌, 张建华, 范娜, 史丽娟. 高粱抗旱性鉴定方法及分子生物学研究进展[J]. 生物技术通报, 2021, 37(4): 260-272.
[10]	张一中, 范昕琦, 杨慧勇, 张晓娟, 邵强, 梁笃, 郭琦, 柳青山, 杜维俊. 基于简化基因组测序高粱育种材料亲缘关系的分析[J]. 生物技术通报, 2020, 36(12): 21-33.
[11]	赵国龙, 林春晶, 金东淳, 张春宝. 主要农作物细胞质雄性不育系育性恢复基因研究进展[J]. 生物技术通报, 2020, 36(1): 116-125.
[12]	张丹, 王楠, 李超, 谢旗, 唐三元. 甜高粱——一种优质的饲料作物[J]. 生物技术通报, 2019, 35(5): 2-8.
[13]	冷传远, 郝怀庆, 景海春. 甜高粱茎秆持汁性研究进展[J]. 生物技术通报, 2019, 35(5): 9-14.
[14]	韩立杰, 才宏伟. 高粱粒重遗传研究进展[J]. 生物技术通报, 2019, 35(5): 15-27.
[15]	丁延庆, 周棱波, 汪灿, 曹宁, 程斌, 高旭, 彭秋, 邵明波, 张立异. 酱香型酒用糯高粱研究进展[J]. 生物技术通报, 2019, 35(5): 28-34.