生物技术通报 ›› 2019, Vol. 35 ›› Issue (9): 1-10.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0614

• 综述与专论 •    下一篇

真菌漆酶的性质、生产、纯化及固定化研究进展

吴怡, 马鸿飞, 曹永佳, 司静, 崔宝凯   

  1. 北京林业大学生态与自然保护学院,北京 100083
  • 收稿日期:2019-07-08 出版日期:2019-09-26 发布日期:2019-09-16
  • 作者简介:吴怡,女,硕士研究生,研究方向:大型真菌漆酶的开发利用;E-mail:1792354604@qq.com
  • 基金资助:
    国家自然科学基金项目(31700016),中央高校基本科研业务费专项资金资助项目(2016ZCQ04)

Advances on Properties,Production,Purification and Immobilization of Fungal Laccase

WU Yi, MA Hong-fei, CAO Yong-jia, SI Jing, CUI Bao-kai   

  1. School of Ecology and Nature Conservation,Beijing Forestry University,Beijing 100083
  • Received:2019-07-08 Published:2019-09-26 Online:2019-09-16

摘要: 真菌漆酶是一种性质优良的多酚氧化酶,由于在分子氧的协助下可将酚类、芳胺类化合物等多种底物氧化,最终得到水及其终产物,符合当代环保工业要求,因而在纸浆漂白、环境治理、生物检测、有机合成等领域有着巨大的应用潜力。就漆酶的生物学性质、生产、纯化、固定化等研究进展和现状进行了介绍和总结,同时对其今后的发展方向进行了展望。

关键词: 漆酶, 生物学性质, 生产, 纯化, 固定化

Abstract: Fungal laccase is a kind of polyphenol oxidase with excellent properties. It can oxidize large amounts of substrates such as phenolic and aromatic amine compounds,coupled with a concomitant reduction of molecular oxygen to water and other final products. Owing to the up-mentioned characteristics,fungal laccase is regarded as a green catalyst for environmental demand,and thus has great application potential in various fields,including pulp bleaching,environmental protection,biological detection,and organic synthesis. In this paper,current advances on biological properties,production,purification,and immobilization of fungal laccase were introduced and summarized,and its future development was prospected.

Key words: laccase, biological properties, production, purification, immobilization