蒽对3株灵芝菌株漆酶活性及其转录表达水平的影响

doi:10.13560/j.cnki.biotech.bull.1985.2019-0260

摘要/Abstract

摘要： 探索多环芳烃（Polycyclic aromatic hydrocarbons,PAHs）蒽对不同灵芝菌株漆酶活性及其转录表达的影响。以3株灵芝菌株（美芝、荣保1号和川芝）为实验材料,避光静置培养5 d后,加入终浓度为1.0 mg/L的蒽,避光处理1 h、3 h、6 h、9 h和24 h,收集上清液测定漆酶活性,菌丝体样品提取RNA,分析多环芳烃蒽对漆酶活性和转录表达水平的影响。结果显示,蒽对3株菌株的漆酶活性及转录表达水平的影响均存在差异。3株灵芝菌株中,川芝的漆酶活性最高,美芝次之,荣保1号酶活最低。荣保1号漆酶活性随着处理时间的延长而上升,培养前期活性被为抑制,后期则被促进;美芝漆酶活性变化趋势类似于荣保1号,变化幅度较小;川芝漆酶活性受到强烈抑制,且随着时间的变化差异不明显。蒽处理下,美芝漆酶基因转录表达水平差异较小,大部分基因在处理9 h后出现转录表达水平峰值;荣保1号漆酶基因的表达水平类似于美芝;川芝漆酶在短期处理下,转录表达水平大幅上调,随着处理时间的延长,转录表达水平下调。3株灵芝菌株漆酶活性和漆酶基因转录表达水平对蒽的响应各不相同,其中川芝较敏感,荣保1号次之,美芝变化幅度最小,耐受力最强。

关键词: 蒽, 灵芝, 转录表达, 漆酶活性

Abstract: This work is to investigate the effects of polycyclic aromatic hydrocarbons（PAHs）on laccase activity and transcriptional expression of different Ganoderma lucidum strains. Three G. lucidum（Meizhi,Rongbao No.1 and Chuanzhi）strains were used as experimental materials. After 5 days of static culture in the dark,a final concentration of 1.0 mg/L anthracene was added. Then the supernatant was collected for the determination of laccase activity after treatment for 1 h,3h,6 h,9 h,and 24 h. Further the mycelium samples were used for RNA extraction,and the effect of anthracene on laccase activity and transcriptional expression of laccase genes was analyzed. The results showed that the effects of anthracene on the laccase activity and transcriptional expression levels of 3 strains differed. Among the 3 strains of G. lucidum,Chuanzhi presented the highest laccase activity,followed by Meizhi,and he lowest activity for Rongbao No. 1. The laccase activity of Rongbao No.1 increased with the prolongation of treatment time,and was inhibited in the early stage of culture,while promoted in the later stage. The change trend of Meizhi’s laccase activity was similar to Rongbao No.1’s,and the change range was small. Enzyme activity of Chuanzhi was strongly inhibited by anthracene and differed insignificantly over time. Under the treatment of anthracene,little variations were observed in the transcript expression levels of Meizhi laccase gene,and most of the genes demonstrated peak transcriptional expression after 9 h treatment. The expression levels of Rongbao No. 1 laccase genes were similar to that of Meizhi. Under short-term treatment,the transcriptional expression level of Chuanzhi laccase genes were significantly up-regulated,and then down-regulated as the treatment time prolonged. In sum,the laccase activities and laccase genes expression levels of 3 strains of G. lucidum varied in response to anthracene;among them,Chuanzhi was the most sensitive,followed by Rongbao No. 1,and Meizhi had the smallest change and thus the strongest tolerance.

Key words: anthracene, Ganoderma lucidum, expression level, laccase activity

胡楚霄, 雷善钰, 秦艳平, 赵奕锦, 向泉桔. 蒽对3株灵芝菌株漆酶活性及其转录表达水平的影响[J]. 生物技术通报, 2019, 35(9): 112-117.

HU Chu-xiao, LEI Shan-yu, QIN Yan-ping, ZHAO Yi-jin, XIANG Quan-ju. Influence of Anthracene on Laccase Activity and Transcriptional Expression Profiles of Ganoderma lucidum[J]. Biotechnology Bulletin, 2019, 35(9): 112-117.

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